{"entity": "researcher", "timestamp": "2026-09-23T15:31:24.261Z", "family": "Unnersj\u00f6-Jess", "given": "David", "initials": "D", "orcid": "0000-0002-4162-0973", "affiliations": ["Science for Life Laboratory, Dept. of Applied Physics, Royal Institute of Technology, Solna, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca"}}, "publications": [{"entity": "publication", "iuid": "3a3a8ff693ed4aa88f61e02c39248a15", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3a3a8ff693ed4aa88f61e02c39248a15.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3a3a8ff693ed4aa88f61e02c39248a15"}}, "title": "Intestinal stroma guides monocyte differentiation to macrophages through GM-CSF.", "authors": [{"family": "Kvedaraite", "given": "Egle", "initials": "E", "orcid": "0000-0001-5308-092X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/12e2bb98f9ea4186a3fc75703b205484.json"}}, {"family": "Lourda", "given": "Magda", "initials": "M", "orcid": "0000-0003-3155-1123", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c3b0f1d58b48430b8553aceb99998d40.json"}}, {"family": "Mouratidou", "given": "Natalia", "initials": "N"}, {"family": "D\u00fcking", "given": "Tim", "initials": "T", "orcid": "0000-0002-4347-7648", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c4b1032e7a34102af444c74bf67dd59.json"}}, {"family": "Padhi", "given": "Avinash", "initials": "A"}, {"family": "Moll", "given": "Kirsten", "initials": "K"}, {"family": "Czarnewski", "given": "Paulo", "initials": "P"}, {"family": "Sinha", "given": "Indranil", "initials": "I", "orcid": "0000-0002-2513-5927", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/83272c13da5c4ea6a8154656d53efb00.json"}}, {"family": "Xagoraris", "given": "Ioanna", "initials": "I"}, {"family": "Kokkinou", "given": "Efthymia", "initials": "E"}, {"family": "Damdimopoulos", "given": "Anastasios", "initials": "A"}, {"family": "Weigel", "given": "Whitney", "initials": "W"}, {"family": "Hartwig", "given": "Olga", "initials": "O", "orcid": "0000-0001-5016-5960", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cbc793bf0f147558140e652a8d2cfa2.json"}}, {"family": "Santos", "given": "Telma E", "initials": "TE"}, {"family": "Soini", "given": "Tea", "initials": "T"}, {"family": "Van Acker", "given": "Aline", "initials": "A"}, {"family": "Rahkonen", "given": "Nelly", "initials": "N"}, {"family": "Flodstr\u00f6m Tullberg", "given": "Malin", "initials": "M", "orcid": "0000-0003-2685-2052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/358926667b494e6d8cc544f34bc80c5e.json"}}, {"family": "Ringqvist", "given": "Emma", "initials": "E"}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cf1bb88eef764f8bb81bdd6d8a231113.json"}}, {"family": "Jorns", "given": "Carl", "initials": "C", "orcid": "0000-0001-7727-8113", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ef68cb234b3141278a29a407ed988b32.json"}}, {"family": "Lindforss", "given": "Ulrik", "initials": "U"}, {"family": "Nordenvall", "given": "Caroline", "initials": "C"}, {"family": "Stamper", "given": "Christopher T", "initials": "CT"}, {"family": "Unnersj\u00f6-Jess", "given": "David", "initials": "D", "orcid": "0000-0002-4162-0973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca.json"}}, {"family": "Akber", "given": "Mira", "initials": "M"}, {"family": "Nadisauskaite", "given": "Ruta", "initials": "R"}, {"family": "Jansson", "given": "Jessica", "initials": "J"}, {"family": "Vandamme", "given": "Niels", "initials": "N"}, {"family": "Sorini", "given": "Chiara", "initials": "C", "orcid": "0000-0002-6803-8377", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/652b92b02a9448179fb0bf7648ed30b9.json"}}, {"family": "Grundeken", "given": "Marijke Elise", "initials": "ME", "orcid": "0000-0001-6915-0339", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/79de930c618c42bbae8e3c7729373d11.json"}}, {"family": "Rolandsdotter", "given": "Helena", "initials": "H"}, {"family": "Rassidakis", "given": "George", "initials": "G"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}, {"family": "Idestr\u00f6m", "given": "Maja", "initials": "M"}, {"family": "Eulitz", "given": "Stefan", "initials": "S"}, {"family": "Arnell", "given": "Henrik", "initials": "H"}, {"family": "Mj\u00f6sberg", "given": "Jenny", "initials": "J", "orcid": "0000-0002-1119-0976", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/33e4f7ea779c4b3382604de7ea259bc8.json"}}, {"family": "Henter", "given": "Jan-Inge", "initials": "JI", "orcid": "0000-0002-0629-2126", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c03e0a2905534e26b5a2a2b5de453872.json"}}, {"family": "Svensson", "given": "Mattias", "initials": "M", "orcid": "0000-0003-1695-7934", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3cb4fb47f3c4be0a6d529a4d8382b82.json"}}], "type": "journal article", "published": "2024-02-26", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "1752", "issn-l": "2041-1723"}, "abstract": "Stromal cells support epithelial cell and immune cell homeostasis and play an important role in inflammatory bowel disease (IBD) pathogenesis. Here, we quantify the stromal response to inflammation in pediatric IBD and reveal subset-specific inflammatory responses across colon segments and intestinal layers. Using data from a murine dynamic gut injury model and human ex vivo transcriptomic, protein and spatial analyses, we report that PDGFRA+CD142-/low fibroblasts and monocytes/macrophages co-localize in the intestine. In primary human fibroblast-monocyte co-cultures, intestinal PDGFRA+CD142-/low fibroblasts foster monocyte transition to CCR2+CD206+ macrophages through granulocyte-macrophage colony-stimulating factor (GM-CSF). Monocyte-derived CCR2+CD206+ cells from co-cultures have a phenotype similar to intestinal CCR2+CD206+ macrophages from newly diagnosed pediatric IBD patients, with high levels of PD-L1 and low levels of GM-CSF receptor. The study describes subset-specific changes in stromal responses to inflammation and suggests that the intestinal stroma guides intestinal macrophage differentiation.", "doi": "10.1038/s41467-024-46076-3", "pmid": "38409190", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10897309"}, {"db": "pii", "key": "10.1038/s41467-024-46076-3"}], "notes": [], "created": "2026-09-23T09:04:49.642Z", "modified": "2026-09-23T09:04:50.297Z"}, {"entity": "publication", "iuid": "bbd214782f1749dc93d1a52a7c6ff8ed", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bbd214782f1749dc93d1a52a7c6ff8ed.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bbd214782f1749dc93d1a52a7c6ff8ed"}}, "title": "Advanced optical imaging reveals preferred spatial orientation of podocyte processes along the axis of glomerular capillaries.", "authors": [{"family": "Unnersj\u00f6-Jess", "given": "David", "initials": "D", "orcid": "0000-0002-4162-0973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca.json"}}, {"family": "Ramdedovic", "given": "Amer", "initials": "A"}, {"family": "Butt", "given": "Linus", "initials": "L"}, {"family": "Plagmann", "given": "Ingo", "initials": "I", "orcid": "0000-0003-0263-7040", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/33bc2ed829db49da9dee963d535fad97.json"}}, {"family": "H\u00f6hne", "given": "Martin", "initials": "M", "orcid": "0000-0001-8698-5389", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f56c4a58c2946bcb5ca08dede681fba.json"}}, {"family": "Hackl", "given": "Agnes", "initials": "A"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "Blom", "given": "Hans", "initials": "H"}, {"family": "Schermer", "given": "Bernhard", "initials": "B"}, {"family": "Benzing", "given": "Thomas", "initials": "T", "orcid": "0000-0003-0512-1066", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f24f9d32cbb84788a18c558e48cac2a8.json"}}], "type": "journal article", "published": "2023-12-00", "journal": {"title": "Kidney Int.", "issn": "1523-1755", "volume": "104", "issue": "6", "pages": "1164-1169", "issn-l": "0085-2538"}, "abstract": "Mammalian kidneys filter enormous volumes of water and small solutes, a filtration driven by the hydrostatic pressure in glomerular capillaries, which is considerably higher than in most other tissues. Interdigitating cellular processes of podocytes form the slits for fluid filtration connected by the membrane-like slit diaphragm cell junction containing a mechanosensitive ion channel complex and allow filtration while counteracting hydrostatic pressure. Several previous publications speculated that podocyte processes may display a preferable orientation on glomerular capillaries instead of a random distribution. However, for decades, the controversy over spatially oriented filtration slits could not be resolved due to technical limitations of imaging technologies. Here, we used advanced high-resolution, three-dimensional microscopy with high data throughput to assess spatial orientation of podocyte processes and filtration slits quantitatively. Filtration-slit-generating secondary processes preferentially align along the capillaries' longitudinal axis while primary processes are preferably perpendicular to the longitudinal direction. This preferential orientation required maturation in development of the mice but was lost in mice with kidney disease due to treatment with nephrotoxic serum or with underlying heterologous mutations in the podocyte foot process protein podocin. Thus, the observation that podocytes maintain a preferred spatial orientation of their processes on glomerular capillaries goes well in line with the role of podocyte foot processes as mechanical buttresses to counteract mechanical forces resulting from pressurized capillaries. Future studies are needed to establish how podocytes establish and maintain their orientation and why orientation is lost under pathological conditions.", "doi": "10.1016/j.kint.2023.08.024", "pmid": "37774923", "labels": [], "xrefs": [{"db": "pii", "key": "S0085-2538(23)00673-7"}], "notes": [], "created": "2026-09-23T12:59:32.194Z", "modified": "2026-09-23T14:49:47.630Z"}, {"entity": "publication", "iuid": "f644cdfffd6a49bf99423f7f92d5d077", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f644cdfffd6a49bf99423f7f92d5d077.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f644cdfffd6a49bf99423f7f92d5d077"}}, "title": "Three-Dimensional Super-Resolved Imaging of Paraffin-Embedded Kidney Samples.", "authors": [{"family": "Unnersj\u00f6-Jess", "given": "David", "initials": "D", "orcid": "0000-0002-4162-0973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca.json"}}, {"family": "Ramdedovic", "given": "Amer", "initials": "A"}, {"family": "H\u00f6hne", "given": "Martin", "initials": "M"}, {"family": "Butt", "given": "Linus", "initials": "L"}, {"family": "Koehler", "given": "Felix C", "initials": "FC"}, {"family": "M\u00fcller", "given": "Roman-Ulrich", "initials": "RU"}, {"family": "Hoyer", "given": "Peter F", "initials": "PF", "orcid": "0000-0002-9132-0282", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b96f1f65d994264b2732e9c70cc4a42.json"}}, {"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b0f7dfe5df1495db2a8386c2c5bb4a5.json"}}, {"family": "Schermer", "given": "Bernhard", "initials": "B"}, {"family": "Benzing", "given": "Thomas", "initials": "T", "orcid": "0000-0003-0512-1066", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f24f9d32cbb84788a18c558e48cac2a8.json"}}], "type": "journal article", "published": "2022-03-31", "journal": {"title": "Kidney360", "issn": "2641-7650", "volume": "3", "issue": "3", "pages": "446-454", "issn-l": null}, "abstract": "Diseases of the glomeruli, the renal filtration units, are a leading cause of progressive kidney disease. Assessment of the ultrastructure of podocytes at the glomerular filtration barrier is essential for diagnosing diverse disease entities, providing insight into the disease pathogenesis, and monitoring treatment responses.\n\nHere we apply previously published sample preparation methods together with stimulated emission depletion and confocal microscopy for resolving nanoscale podocyte substructure. The protocols are modified and optimized in order to be applied to formalin-fixed paraffin-embedded (FFPE) samples.\n\nWe successfully modified our protocols to allow for deep three-dimensional stimulated emission depletion and confocal imaging of FFPE kidney tissue with similar staining and image quality compared with our previous approaches. We further show that quantitative analysis can be applied to extract morphometrics from healthy and diseased samples from both mice and humans.\n\nThe results from this study could increase the feasibility of implementing optical kidney imaging protocols in clinical routines because FFPE is the gold-standard method for storage of patient samples.", "doi": "10.34067/KID.0005882021", "pmid": "35582181", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9034812"}, {"db": "pii", "key": "02200512-202203000-00009"}], "notes": [], "created": "2026-09-23T11:19:15.519Z", "modified": "2026-09-23T11:19:15.700Z"}, {"entity": "publication", "iuid": "4b3db8c0d0dc472d8c208b3ec381f251", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4b3db8c0d0dc472d8c208b3ec381f251.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4b3db8c0d0dc472d8c208b3ec381f251"}}, "title": "Super-Resolution Imaging of the Filtration Barrier Suggests a Role for Podocin R229Q in Genetic Predisposition to Glomerular Disease.", "authors": [{"family": "Butt", "given": "Linus", "initials": "L"}, {"family": "Unnersj\u00f6-Jess", "given": "David", "initials": "D", "orcid": "0000-0002-4162-0973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca.json"}}, {"family": "H\u00f6hne", "given": "Martin", "initials": "M", "orcid": "0000-0001-8698-5389", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f56c4a58c2946bcb5ca08dede681fba.json"}}, {"family": "Hahnfeldt", "given": "Robert", "initials": "R"}, {"family": "Reilly", "given": "Dervla", "initials": "D", "orcid": "0000-0002-9456-1364", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d0b98112f2c438aa42a4af98e58d712.json"}}, {"family": "Rinschen", "given": "Markus M", "initials": "MM"}, {"family": "Plagmann", "given": "Ingo", "initials": "I", "orcid": "0000-0003-0263-7040", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/33bc2ed829db49da9dee963d535fad97.json"}}, {"family": "Diefenhardt", "given": "Paul", "initials": "P"}, {"family": "Br\u00e4hler", "given": "Sebastian", "initials": "S"}, {"family": "Brinkk\u00f6tter", "given": "Paul T", "initials": "PT"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "Blom", "given": "Hans", "initials": "H"}, {"family": "Schermer", "given": "Bernhard", "initials": "B"}, {"family": "Benzing", "given": "Thomas", "initials": "T", "orcid": "0000-0003-0512-1066", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f24f9d32cbb84788a18c558e48cac2a8.json"}}], "type": "journal article", "published": "2022-01-00", "journal": {"title": "J. Am. Soc. Nephrol.", "issn": "1533-3450", "volume": "33", "issue": "1", "pages": "138-154", "issn-l": "1046-6673"}, "abstract": "Diseases of the kidney's glomerular filtration barrier are a leading cause of end stage renal failure. Despite a growing understanding of genes involved in glomerular disorders in children, the vast majority of adult patients lack a clear genetic diagnosis. The protein podocin p.R229Q, which results from the most common missense variant in NPHS2, is enriched in cohorts of patients with FSGS. However, p.R229Q has been proposed to cause disease only when transassociated with specific additional genetic alterations, and population-based epidemiologic studies on its association with albuminuria yielded ambiguous results.\n\nTo test whether podocin p.R229Q may also predispose to the complex disease pathogenesis in adults, we introduced the exact genetic alteration in mice using CRISPR/Cas9-based genome editing (Pod ). We assessed the phenotype using super-resolution microscopy and albuminuria measurements and evaluated the stability of the mutant protein in cell culture experiments.R231Q\n\nHeterozygous Pod mice did not present any overt kidney disease or proteinuria. However, homozygous R231Q/wild-typePod mice developed increased levels of albuminuria with age, and super-resolution microscopy revealed preceding ultrastructural morphologic alterations that were recently linked to disease predisposition. When injected with nephrotoxic serum to induce glomerular injury, heterozygous R231Q/R231QPod mice showed a more severe course of disease compared with R231Q/wild-typePod mice. Podocin protein levels were decreased in wild-type/wild-typePod and R231Q/wild-typePod mice as well as in human cultured podocytes expressing the podocinR231Q/R231QR231Q variant. Our in vitro experiments indicate an underlying increased proteasomal degradation.\n\nOur findings demonstrate that podocin R231Q exerts a pathogenic effect on its own, supporting the concept of podocin R229Q contributing to genetic predisposition in adult patients.", "doi": "10.1681/ASN.2020060858", "pmid": "34853150", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8763184"}, {"db": "pii", "key": "00001751-202201000-00016"}], "notes": [], "created": "2026-09-23T12:01:19.209Z", "modified": "2026-09-23T14:51:18.858Z"}]}