{"entity": "researcher", "timestamp": "2026-08-20T20:48:57.817Z", "family": "Brandt", "given": "Ludwig", "initials": "L", "orcid": "0000-0003-2040-3176", "affiliations": ["Department of Applied Physics, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. sniklas@kth.se."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/328e28faa0794e95a4e6cd96e2508ad0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/328e28faa0794e95a4e6cd96e2508ad0"}}, "publications": [{"entity": "publication", "iuid": "9be7cc03b2c44b2eae7610a760cb73f4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9be7cc03b2c44b2eae7610a760cb73f4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9be7cc03b2c44b2eae7610a760cb73f4"}}, "title": "Allelic variation of KIR and HLA tunes the cytolytic payload and determines functional hierarchy of NK cell repertoires.", "authors": [{"family": "Philippon", "given": "Camille", "initials": "C"}, {"family": "Tao", "given": "Sudan", "initials": "S"}, {"family": "Clement", "given": "Dennis", "initials": "D"}, {"family": "Haroun-Izquierdo", "given": "Alvaro", "initials": "A", "orcid": "0000-0003-4557-3606", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3ce9327b5d1b4c3f9fc91fd289cea6a8.json"}}, {"family": "Kichula", "given": "Katherine M", "initials": "KM", "orcid": "0000-0002-9817-3043", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/72b325af2be24f73b2343a0f6c3c87a6.json"}}, {"family": "Netskar", "given": "Herman", "initials": "H", "orcid": "0000-0002-3081-9581", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5dd8d11bf80a400ea9b92a8fa0c1b2d6.json"}}, {"family": "Brandt", "given": "Ludwig", "initials": "L", "orcid": "0000-0003-2040-3176", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/328e28faa0794e95a4e6cd96e2508ad0.json"}}, {"family": "Oei", "given": "Vincent Sheng", "initials": "VS", "orcid": "0000-0002-9336-529X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1b56943879714e6d9483c306ddb7587a.json"}}, {"family": "Kanaya", "given": "Minoru", "initials": "M", "orcid": "0000-0002-0035-8657", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/035eb746532c4679a1d85116dfd75d98.json"}}, {"family": "Lanuza", "given": "Pilar Maria", "initials": "PM"}, {"family": "Schaffer", "given": "Marie", "initials": "M"}, {"family": "Goodridge", "given": "Jodie P", "initials": "JP", "orcid": "0000-0001-7531-6032", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c5adc441b4dd4567992da116d4faa53a.json"}}, {"family": "Horowitz", "given": "Amir", "initials": "A"}, {"family": "Zhu", "given": "Faming", "initials": "F", "orcid": "0000-0002-1963-9176", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c170188e089c420392583603a8af8c2d.json"}}, {"family": "Hammer", "given": "Quirin", "initials": "Q", "orcid": "0000-0003-2968-6061", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bd1d59f056c149db98064ff1b0b99ed6.json"}}, {"family": "Sohlberg", "given": "Ebba", "initials": "E"}, {"family": "Majhi", "given": "Rakesh Kumar", "initials": "RK"}, {"family": "Kveberg", "given": "Lise", "initials": "L", "orcid": "0000-0001-9308-0641", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65d8133f4b424945b42e3dee2f4c2493.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Norman", "given": "Paul J", "initials": "PJ"}, {"family": "Malmberg", "given": "Karl-Johan", "initials": "KJ", "orcid": "0000-0002-8718-9373", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1b891c497ca402aaff79e8082f22553.json"}}], "type": "journal article", "published": "2023-08-22", "journal": {"title": "Blood Adv", "issn": "2473-9529", "volume": "7", "issue": "16", "pages": "4492-4504", "issn-l": null}, "abstract": "The functionality of natural killer (NK) cells is tuned during education and is associated with remodeling of the lysosomal compartment. We hypothesized that genetic variation in killer cell immunoglobulin-like receptor (KIR) and HLA, which is known to influence the functional strength of NK cells, fine-tunes the payload of effector molecules stored in secretory lysosomes. To address this possibility, we performed a high-resolution analysis of KIR and HLA class I genes in 365 blood donors and linked genotypes to granzyme B loading and functional phenotypes. We found that granzyme B levels varied across individuals but were stable over time in each individual and genetically determined by allelic variation in HLA class I genes. A broad mapping of surface receptors and lysosomal effector molecules revealed that DNAM-1 and granzyme B levels served as robust metric of the functional state in NK cells. Variation in granzyme B levels at rest was tightly linked to the lytic hit and downstream killing of major histocompatibility complex-deficient target cells. Together, these data provide insights into how variation in genetically hardwired receptor pairs tunes the releasable granzyme B pool in NK cells, resulting in predictable hierarchies in global NK cell function.", "doi": "10.1182/bloodadvances.2023009827", "pmid": "37327114", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10440473"}, {"db": "pii", "key": "496303"}], "notes": [], "created": "2026-08-20T12:18:49.188Z", "modified": "2026-08-20T12:18:49.482Z"}, {"entity": "publication", "iuid": "93affd71ae484063aa0e659fb984e380", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/93affd71ae484063aa0e659fb984e380.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/93affd71ae484063aa0e659fb984e380"}}, "title": "Live single cell imaging assays in glass microwells produced by laser-induced deep etching.", "authors": [{"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N", "orcid": "0000-0001-6443-878X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b65278c9896e466f92f62f3ba1e58136.json"}}, {"family": "Brandt", "given": "Ludwig", "initials": "L", "orcid": "0000-0003-2040-3176", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/328e28faa0794e95a4e6cd96e2508ad0.json"}}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Zambarda", "given": "Chiara", "initials": "C"}, {"family": "Guldevall", "given": "Karolin", "initials": "K"}, {"family": "Schulz-Ruhtenberg", "given": "Malte", "initials": "M"}, {"family": "R\u00f6sener", "given": "Bernd", "initials": "B"}, {"family": "Kr\u00fcger", "given": "Robin A", "initials": "RA"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}], "type": "journal article", "published": "2022-05-31", "journal": {"title": "Lab Chip", "issn": "1473-0189", "volume": "22", "issue": "11", "pages": "2107-2121", "issn-l": null}, "abstract": "Miniaturization of cell culture substrates enables controlled analysis of living cells in confined micro-scale environments. This is particularly suitable for imaging individual cells over time, as they can be monitored without escaping the imaging field-of-view (FoV). Glass materials are ideal for most microscopy applications. However, with current methods used in life sciences, glass microfabrication is limited in terms of either freedom of design, quality, or throughput. In this work, we introduce laser-induced deep etching (LIDE) as a method for producing glass microwell arrays for live single cell imaging assays. We demonstrate novel microwell arrays with deep, high-aspect ratio wells that have rounded, dimpled or flat bottom profiles in either single-layer or double-layer glass chips. The microwells are evaluated for microscopy-based analysis of long-term cell culture, clonal expansion, laterally organized cell seeding, subcellular mechanics during migration and immune cell cytotoxicity assays of both adherent and suspension cells. It is shown that all types of microwells can support viable cell cultures and imaging with single cell resolution, and we highlight specific benefits of each microwell design for different applications. We believe that high-quality glass microwell arrays enabled by LIDE provide a great option for high-content and high-resolution imaging-based live cell assays with a broad range of potential applications within life sciences.", "doi": "10.1039/d2lc00090c", "pmid": "35470832", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T09:27:30.995Z", "modified": "2026-08-20T09:27:31.118Z"}]}