{"entity": "researcher", "timestamp": "2026-08-26T22:47:33.749Z", "family": "Leroux", "given": "Louis Philippe", "initials": "LP", "orcid": "0000-0002-9736-1827", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/2eae6a7fbec644f9ae9b34069c03d845.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/2eae6a7fbec644f9ae9b34069c03d845"}}, "publications": [{"entity": "publication", "iuid": "2d729c64be074b4e8ecb1276015c75dc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2d729c64be074b4e8ecb1276015c75dc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2d729c64be074b4e8ecb1276015c75dc"}}, "title": "Translational profiling of macrophages infected with Leishmania donovani identifies mTOR- and eIF4A-sensitive immune-related transcripts.", "authors": [{"family": "Chaparro", "given": "Visnu", "initials": "V", "orcid": "0000-0001-6880-9234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c6b79e01f4b445abb58d7d2ce56df0a.json"}}, {"family": "Leroux", "given": "Louis-Philippe", "initials": "LP", "orcid": "0000-0002-9736-1827", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2eae6a7fbec644f9ae9b34069c03d845.json"}}, {"family": "Masvidal", "given": "Laia", "initials": "L", "orcid": "0000-0002-0460-3058", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d07095bf51044db4ad6e77b94c9ad2c3.json"}}, {"family": "Lorent", "given": "Julie", "initials": "J"}, {"family": "Graber", "given": "Tyson E", "initials": "TE", "orcid": "0000-0002-5337-3404", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3b0557c0b434e328406d11eb04cfd43.json"}}, {"family": "Zimmermann", "given": "Aude", "initials": "A", "orcid": "0000-0001-5908-5190", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1be55e1617548ebb73844878efd485d.json"}}, {"family": "Arango Duque", "given": "Guillermo", "initials": "G", "orcid": "0000-0002-1216-2571", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c8bf0a5cb7624e63962c9ef421de0146.json"}}, {"family": "Descoteaux", "given": "Albert", "initials": "A", "orcid": "0000-0002-0633-5309", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/138c80938416451d95ff9a850f57d2c9.json"}}, {"family": "Alain", "given": "Tommy", "initials": "T"}, {"family": "Larsson", "given": "Ola", "initials": "O"}, {"family": "Jaramillo", "given": "Maritza", "initials": "M", "orcid": "0000-0002-1910-5684", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ac29241def64821998ecaa60a3783f4.json"}}], "type": "journal article", "published": "2020-06-00", "journal": {"title": "PLoS Pathog", "issn": "1553-7374", "volume": "16", "issue": "6", "pages": "e1008291", "issn-l": "1553-7366"}, "abstract": "The protozoan parasite Leishmania donovani (L. donovani) causes visceral leishmaniasis, a chronic infection which is fatal when untreated. Herein, we investigated whether in addition to altering transcription, L. donovani modulates host mRNA translation to establish a successful infection. Polysome-profiling revealed that one third of protein-coding mRNAs expressed in primary mouse macrophages are differentially translated upon infection with L. donovani promastigotes or amastigotes. Gene ontology analysis identified key biological processes enriched for translationally regulated mRNAs and were predicted to be either activated (e.g. chromatin remodeling and RNA metabolism) or inhibited (e.g. intracellular trafficking and antigen presentation) upon infection. Mechanistic in silico and biochemical analyses showed selective activation mTOR- and eIF4A-dependent mRNA translation, including transcripts encoding central regulators of mRNA turnover and inflammation (i.e. PABPC1, EIF2AK2, and TGF-\u03b2). L. donovani survival within macrophages was favored under mTOR inhibition but was dampened by pharmacological blockade of eIF4A. Overall, this study uncovers a vast yet selective reprogramming of the host cell translational landscape early during L. donovani infection, and suggests that some of these changes are involved in host defense mechanisms while others are part of parasite-driven survival strategies. Further in vitro and in vivo investigation will shed light on the contribution of mTOR- and eIF4A-dependent translational programs to the outcome of visceral leishmaniasis.", "doi": "10.1371/journal.ppat.1008291", "pmid": "32479529", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7310862"}, {"db": "pii", "key": "PPATHOGENS-D-19-02315"}], "notes": [], "created": "2026-08-20T12:44:45.458Z", "modified": "2026-08-20T12:44:45.559Z"}, {"entity": "publication", "iuid": "c2c2b2f980374175ae6d51c6a6d8e586", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c2c2b2f980374175ae6d51c6a6d8e586.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c2c2b2f980374175ae6d51c6a6d8e586"}}, "title": "Translational profiling of macrophages infected with Leishmania donovani identifies mTOR- and eIF4A-sensitive immune-related transcripts", "authors": [{"family": "Chaparro", "given": "Visnu", "initials": "V", "orcid": "0000-0001-6880-9234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c6b79e01f4b445abb58d7d2ce56df0a.json"}}, {"family": "Leroux", "given": "Louis Philippe", "initials": "LP", "orcid": "0000-0002-9736-1827", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2eae6a7fbec644f9ae9b34069c03d845.json"}}, {"family": "Masvidal", "given": "Laia", "initials": "L"}, {"family": "Lorent", "given": "Julie", "initials": "J"}, {"family": "Graber", "given": "Tyson E", "initials": "TE", "orcid": "0000-0002-5337-3404", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3b0557c0b434e328406d11eb04cfd43.json"}}, {"family": "Zimmermann", "given": "Aude", "initials": "A", "orcid": "0000-0001-5908-5190", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1be55e1617548ebb73844878efd485d.json"}}, {"family": "Duque", "given": "Guillermo Arango", "initials": "GA", "orcid": "0000-0002-1216-2571", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c8bf0a5cb7624e63962c9ef421de0146.json"}}, {"family": "Descoteaux", "given": "Albert", "initials": "A", "orcid": "0000-0002-0633-5309", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/138c80938416451d95ff9a850f57d2c9.json"}}, {"family": "Alain", "given": "Tommy", "initials": "T"}, {"family": "Larsson", "given": "Ola", "initials": "O"}, {"family": "Jaramillo", "given": "Maritza", "initials": "M", "orcid": "0000-0002-1910-5684", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ac29241def64821998ecaa60a3783f4.json"}}], "type": "posted-content", "published": "2019-12-20", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2019.12.20.884338", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T09:55:13.814Z", "modified": "2026-08-20T09:55:14.108Z"}, {"entity": "publication", "iuid": "06be6762bc3e4702920601ce6ef7f28c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/06be6762bc3e4702920601ce6ef7f28c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/06be6762bc3e4702920601ce6ef7f28c"}}, "title": "The Protozoan Parasite Toxoplasma gondii Selectively Reprograms the Host Cell Translatome.", "authors": [{"family": "Leroux", "given": "Louis-Philippe", "initials": "LP", "orcid": "0000-0002-9736-1827", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2eae6a7fbec644f9ae9b34069c03d845.json"}}, {"family": "Lorent", "given": "Julie", "initials": "J"}, {"family": "Graber", "given": "Tyson E", "initials": "TE"}, {"family": "Chaparro", "given": "Visnu", "initials": "V"}, {"family": "Masvidal", "given": "Laia", "initials": "L"}, {"family": "Aguirre", "given": "Maria", "initials": "M"}, {"family": "Fonseca", "given": "Bruno D", "initials": "BD"}, {"family": "van Kempen", "given": "L\u00e9on C", "initials": "LC"}, {"family": "Alain", "given": "Tommy", "initials": "T"}, {"family": "Larsson", "given": "Ola", "initials": "O"}, {"family": "Jaramillo", "given": "Maritza", "initials": "M", "orcid": "0000-0002-1910-5684", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ac29241def64821998ecaa60a3783f4.json"}}], "type": "journal article", "published": "2018-09-00", "journal": {"title": "Infect. Immun.", "issn": "1098-5522", "volume": "86", "issue": "9", "issn-l": "0019-9567"}, "abstract": "The intracellular parasite Toxoplasma gondii promotes infection by targeting multiple host cell processes; however, whether it modulates mRNA translation is currently unknown. Here, we show that infection of primary murine macrophages with type I or II T. gondii strains causes a profound perturbation of the host cell translatome. Notably, translation of transcripts encoding proteins involved in metabolic activity and components of the translation machinery was activated upon infection. In contrast, the translational efficiency of mRNAs related to immune cell activation and cytoskeleton/cytoplasm organization was largely suppressed. Mechanistically, T. gondii bolstered mechanistic target of rapamycin (mTOR) signaling to selectively activate the translation of mTOR-sensitive mRNAs, including those with a 5'-terminal oligopyrimidine (5' TOP) motif and those encoding mitochondrion-related proteins. Consistent with parasite modulation of host mTOR-sensitive translation to promote infection, inhibition of mTOR activity suppressed T. gondii replication. Thus, selective reprogramming of host mRNA translation represents an important subversion strategy during T. gondii infection.", "doi": "10.1128/IAI.00244-18", "pmid": "29967092", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6105892"}, {"db": "pii", "key": "IAI.00244-18"}], "notes": [], "created": "2019-01-17T13:59:43.975Z", "modified": "2026-08-21T09:30:12.809Z"}]}