{"entity": "researcher", "timestamp": "2026-09-25T23:04:04.282Z", "family": "\u0160achl", "given": "Radek", "initials": "R", "orcid": "0000-0002-0441-3908", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c67ca29f2094890a807e6c04ca0988b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c67ca29f2094890a807e6c04ca0988b"}}, "publications": [{"entity": "publication", "iuid": "c290c3d26ce5430c844e21cf540841ba", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c290c3d26ce5430c844e21cf540841ba.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c290c3d26ce5430c844e21cf540841ba"}}, "title": "Revised Diffusion Law Permits Quantitative Nanoscale Characterization of Membrane Organization.", "authors": [{"family": "Svobodov\u00e1", "given": "Barbora", "initials": "B"}, {"family": "\u0160t'astn\u00fd", "given": "David", "initials": "D"}, {"family": "Blom", "given": "Hans", "initials": "H"}, {"family": "Mikhalyov", "given": "Ilya", "initials": "I"}, {"family": "Gretskaya", "given": "Natalia", "initials": "N"}, {"family": "Ballekov\u00e1", "given": "Alena", "initials": "A", "orcid": "0000-0002-4969-8361", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f59ee629f7b4bdf8a02d462571326f6.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7bd3619c7fd48318bc4c1e7a9910df8.json"}}, {"family": "Hof", "given": "Martin", "initials": "M", "orcid": "0000-0003-2884-3037", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0785c18804f049b09fd09c498ae13539.json"}}, {"family": "\u0160achl", "given": "Radek", "initials": "R", "orcid": "0000-0002-0441-3908", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c67ca29f2094890a807e6c04ca0988b.json"}}], "type": "journal article", "published": "2025-06-10", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "97", "issue": "22", "pages": "11478-11485", "issn-l": "0003-2700"}, "abstract": "The formation of functional nanoscopic domains is an inherent property of plasma membranes. Stimulated emission depletion combined with fluorescence correlation spectroscopy (STED-FCS) has been previously used to identify such domains; however, the information obtained by STED-FCS has been limited to the presence of such domains while crucial parameters have not been accessible, such as size (Rd), the fraction of occupied membrane surface (f), in-membrane lipid diffusion inside (Din) and outside (Dout) the nanodomains as well as their self-diffusion (Dd). Here, we introduce a quantitative approach based on a revised interpretation of the diffusion law. By analyzing experimentally recorded STED-FCS diffusion law plots using a comprehensive library of simulated diffusion law plots, we extract these five parameters from STED-FCS data. That approach is verified on ganglioside nanodomains in giant unilamellar vesicles, validating the Saffman-Delbr\u00fcck assumption for Dd. STED-FCS data in both plasma membranes of living PtK2 cells and giant plasma membrane vesicles are examined, and a quantitative framework for molecular diffusion modes in biological membranes is presented.", "doi": "10.1021/acs.analchem.5c00021", "pmid": "40437882", "labels": {"SciLifeLab Fellow": "", "Erdinc Sezgin": ""}, "xrefs": [{"db": "pmc", "key": "PMC12163893"}], "notes": [], "created": "2026-09-23T15:47:56.438Z", "modified": "2026-09-23T15:47:56.493Z"}, {"entity": "publication", "iuid": "4d8f4f55fc4f410f8d14389fe77ff282", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4d8f4f55fc4f410f8d14389fe77ff282.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4d8f4f55fc4f410f8d14389fe77ff282"}}, "title": "Revised diffusion law permits quantitative nanoscale characterization of membrane organization", "authors": [{"family": "Svobodov\u00e1", "given": "Barbora", "initials": "B", "orcid": "0000-0002-1796-7408", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65bb61d5fe2b42e084635018add3d7fb.json"}}, {"family": "\u0160\u0165astn\u00fd", "given": "David", "initials": "D", "orcid": "0009-0008-8898-3377", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/39431ab745bc4ea8a138f3fac2e96b71.json"}}, {"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b0f7dfe5df1495db2a8386c2c5bb4a5.json"}}, {"family": "Mikhalyov", "given": "Ilya", "initials": "I"}, {"family": "Gretskaya", "given": "Natalia", "initials": "N", "orcid": "0000-0002-1332-9396", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/15fdf56e56c24383abacdfd578b6c69a.json"}}, {"family": "Ballekov\u00e1", "given": "Alena", "initials": "A", "orcid": "0000-0002-4969-8361", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f59ee629f7b4bdf8a02d462571326f6.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7bd3619c7fd48318bc4c1e7a9910df8.json"}}, {"family": "Hof", "given": "Martin", "initials": "M", "orcid": "0000-0003-2884-3037", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0785c18804f049b09fd09c498ae13539.json"}}, {"family": "\u0160achl", "given": "Radek", "initials": "R", "orcid": "0000-0002-0441-3908", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c67ca29f2094890a807e6c04ca0988b.json"}}], "type": "posted-content", "published": "2024-11-03", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.11.01.621464", "pmid": null, "labels": {"SciLifeLab Fellow": "", "Erdinc Sezgin": ""}, "xrefs": [], "notes": [], "created": "2026-09-23T14:52:22.372Z", "modified": "2026-09-23T14:52:22.606Z"}]}