{"entity": "researcher", "timestamp": "2026-07-22T17:15:34.390Z", "family": "Vicidomini", "given": "Giuseppe", "initials": "G", "orcid": "0000-0002-3085-730X", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/298bc4bbc62948dc8f798723a09a2df8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/298bc4bbc62948dc8f798723a09a2df8"}}, "publications": [{"entity": "publication", "iuid": "d296c422a77b45b485e233adca7f207b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d296c422a77b45b485e233adca7f207b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d296c422a77b45b485e233adca7f207b"}}, "title": "Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo.", "authors": [{"family": "Dreier", "given": "Jes", "initials": "J", "orcid": "0000-0002-9596-5125", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c35d3a4590d74885a9a0580ff0d2fbb2.json"}}, {"family": "Castello", "given": "Marco", "initials": "M"}, {"family": "Coceano", "given": "Giovanna", "initials": "G"}, {"family": "C\u00e1ceres", "given": "Rodrigo", "initials": "R"}, {"family": "Plastino", "given": "Julie", "initials": "J"}, {"family": "Vicidomini", "given": "Giuseppe", "initials": "G", "orcid": "0000-0002-3085-730X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/298bc4bbc62948dc8f798723a09a2df8.json"}}, {"family": "Testa", "given": "Ilaria", "initials": "I"}], "type": "journal article", "published": "2019-02-01", "journal": {"title": "Nat Commun", "issn": "2041-1723", "issn-l": "2041-1723", "volume": "10", "issue": "1", "pages": "556"}, "abstract": "RESOLFT fluorescence nanoscopy can nowadays image details far beyond the diffraction limit. However, signal to noise ratio (SNR) and temporal resolution are still a concern, especially deep inside living cells and organisms. In this work, we developed a non-deterministic scanning approach based on a real-time feedback system which speeds up the acquisition up to 6-fold and decreases the light dose by 70-90% for in vivo imaging. Also, we extended the information content of the images by acquiring the complete temporal evolution of the fluorescence generated by reversible switchable fluorescent proteins. This generates a series of images with different spatial resolution and SNR, from conventional to RESOLFT images, which combined through a multi-image deconvolution algorithm further enhances the effective resolution. We reported nanoscale imaging of organelles up to 35 Hz and actin dynamics during an invasion process at a depth of 20-30 \u00b5m inside a living Caenorhabditis elegans worm.", "doi": "10.1038/s41467-019-08442-4", "pmid": "30710076", "labels": {"Ilaria Testa": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-019-08442-4"}, {"db": "pmc", "key": "PMC6358637"}], "notes": [], "created": "2020-09-29T10:04:14.117Z", "modified": "2022-11-04T11:32:16.822Z"}, {"entity": "publication", "iuid": "e488a810ba184886b518f887528906aa", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e488a810ba184886b518f887528906aa.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e488a810ba184886b518f887528906aa"}}, "title": "Non-Linear Microscopy", "authors": [{"family": "Mazza", "given": "D", "initials": "D"}, {"family": "Bianchini", "given": "P", "initials": "P"}, {"family": "Caorsi", "given": "V", "initials": "V"}, {"family": "Cella", "given": "F", "initials": "F"}, {"family": "Mondal", "given": "P P", "initials": "PP"}, {"family": "Ronzitti", "given": "E", "initials": "E"}, {"family": "Testa", "given": "I", "initials": "I", "orcid": "0000-0003-4005-4997", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e8c6a5d1e3a479e933eb8f143b404fc.json"}}, {"family": "Vicidomini", "given": "G", "initials": "G", "orcid": "0000-0002-3085-730X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/298bc4bbc62948dc8f798723a09a2df8.json"}}, {"family": "Diaspro", "given": "A", "initials": "A"}], "type": "book-chapter", "published": "2008-08-13", "journal": {"title": "Springer", "issn": "1618-7210", "volume": "[book chapter]", "issue": null, "pages": "47-69", "issn-l": null}, "abstract": null, "doi": "10.1007/978-3-540-76782-4_4", "pmid": null, "labels": {"Ilaria Testa": null, "SciLifeLab Fellow": null}, "xrefs": [], "notes": "In Pavesi L., Fauchet P.M. (eds) Biophotonics. Biological and Medical Physics, Biomedical Engineering.  \r\nPrint ISBN 978-3-540-76779-4  \r\nOnline ISBN 978-3-540-76782-4", "created": "2020-10-12T10:31:27.602Z", "modified": "2025-12-04T17:04:40.051Z"}]}