{"entity": "researcher", "timestamp": "2026-08-22T06:57:52.346Z", "family": "Skorupinska-Tudek", "given": "Karolina", "initials": "K", "orcid": "0000-0002-3472-2517", "affiliations": ["Laboratory of Lipid Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/292def4b4fc24eca9d59d69f1ad5404d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/292def4b4fc24eca9d59d69f1ad5404d"}}, "publications": [{"entity": "publication", "iuid": "cbb5e9abfa794b28b00cc9b11c3d87f7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/cbb5e9abfa794b28b00cc9b11c3d87f7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/cbb5e9abfa794b28b00cc9b11c3d87f7"}}, "title": "Potentiating V\u03b39V\u03b42 T cell proliferation and assessing their cytotoxicity towards adherent cancer cells at the single cell level.", "authors": [{"family": "Liu", "given": "Chenxiao", "initials": "C", "orcid": "0000-0002-5349-2366", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f453d2233da54888aaae13669751ba14.json"}}, {"family": "Skorupinska-Tudek", "given": "Karolina", "initials": "K", "orcid": "0000-0002-3472-2517", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/292def4b4fc24eca9d59d69f1ad5404d.json"}}, {"family": "Eriksson", "given": "Sven-G\u00f6ran", "initials": "SG"}, {"family": "Parmryd", "given": "Ingela", "initials": "I", "orcid": "0000-0003-4834-3611", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1279c810d97d45c4a7c4ae460acb2840.json"}}], "type": "journal article", "published": "2022-02-15", "journal": {"title": "Biol Open", "issn": "2046-6390", "volume": "11", "issue": "1", "issn-l": "2046-6390"}, "abstract": "V\u03b39V\u03b42 T cells is the dominant \u03b3\u03b4 T cell subset in human blood. They are cytotoxic and activated by phosphoantigens whose concentrations are increased in cancer cells, making the cancer cells targets for V\u03b39V\u03b42 T cell immunotherapy. For successful immunotherapy, it is important both to characterise V\u03b39V\u03b42 T cell proliferation and optimise the assessment of their cytotoxic potential, which is the aim of this study. We found that supplementation with freshly thawed human serum potentiated V\u03b39V\u03b42 T cell proliferation from peripheral mononuclear cells (PBMCs) stimulated with (E)-4-Hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) and consistently enabled V\u03b39V\u03b42 T cell proliferation from cryopreserved PBMCs. In cryopreserved PBMCs the proliferation was higher than in freshly prepared PBMCs. In a panel of short-chain prenyl alcohols, monophosphates and diphosphates, most diphosphates and also dimethylallyl monophosphate stimulated V\u03b39V\u03b42 T cell proliferation. We developed a method where the cytotoxicity of V\u03b39V\u03b42 T cells towards adherent cells is assessed at the single cell level using flow cytometry, which gives more clear-cut results than the traditional bulk release assays. Moreover, we found that HMBPP enhances the V\u03b39V\u03b42 T cell cytotoxicity towards colon cancer cells. In summary, we have developed an easily interpretable method to assess the cytotoxicity of V\u03b39V\u03b42 T cells towards adherent cells, found that V\u03b39V\u03b42 T cell proliferation can be potentiated by media-supplementation and how misclassification of non-responders may be avoided. Our findings will be useful in the further development of V\u03b39V\u03b42 T cell immunotherapy.", "doi": "10.1242/bio.059049", "pmid": "34994391", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8822357"}, {"db": "pii", "key": "274281"}], "notes": [], "created": "2026-08-21T12:42:19.695Z", "modified": "2026-08-21T12:42:19.862Z"}]}