{"entity": "researcher", "timestamp": "2026-09-27T17:02:51.731Z", "family": "Blom", "given": "Sami", "initials": "S", "orcid": "0000-0002-1878-8911", "affiliations": ["Institute for Molecular Medicine Finland FIMM, University of Helsinki, Helsinki, Finland."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/29223b758c654de896c5ed1addf3e3a9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/29223b758c654de896c5ed1addf3e3a9"}}, "publications": [{"entity": "publication", "iuid": "30d422f662284715b46aa65857e9753c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/30d422f662284715b46aa65857e9753c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/30d422f662284715b46aa65857e9753c"}}, "title": "ITGB1-dependent upregulation of Caveolin-1 switches TGF\u03b2 signalling from tumour-suppressive to oncogenic in prostate cancer.", "authors": [{"family": "Pellinen", "given": "Teijo", "initials": "T"}, {"family": "Blom", "given": "Sami", "initials": "S", "orcid": "0000-0002-1878-8911", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/29223b758c654de896c5ed1addf3e3a9.json"}}, {"family": "S\u00e1nchez", "given": "Sara", "initials": "S"}, {"family": "V\u00e4lim\u00e4ki", "given": "Katja", "initials": "K"}, {"family": "Mpindi", "given": "John-Patrick", "initials": "JP"}, {"family": "Azegrouz", "given": "Hind", "initials": "H"}, {"family": "Strippoli", "given": "Raffaele", "initials": "R"}, {"family": "Nieto", "given": "Raquel", "initials": "R"}, {"family": "Vit\u00f3n", "given": "Mariano", "initials": "M"}, {"family": "Palacios", "given": "Irene", "initials": "I"}, {"family": "Turkki", "given": "Riku", "initials": "R", "orcid": "0000-0002-8690-6983", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/74597043811c4f49bfe09812a9a5f3a4.json"}}, {"family": "Wang", "given": "Yinhai", "initials": "Y", "orcid": "0000-0002-3671-4932", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e036bbd65b6f497796c0f4f097c4ea47.json"}}, {"family": "S\u00e1nchez-Alvarez", "given": "Miguel", "initials": "M"}, {"family": "Nordling", "given": "Stig", "initials": "S"}, {"family": "B\u00fctzow", "given": "Anna", "initials": "A"}, {"family": "Mirtti", "given": "Tuomas", "initials": "T", "orcid": "0000-0003-0455-9891", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1963bc5086f4b45b9a48cb60115d578.json"}}, {"family": "Rannikko", "given": "Antti", "initials": "A"}, {"family": "Montoya", "given": "Mar\u00eda C", "initials": "MC", "orcid": "0000-0001-8878-7028", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/621410483b524d2a90ee212486514894.json"}}, {"family": "Kallioniemi", "given": "Olli", "initials": "O", "orcid": "0000-0002-3231-0332", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb8605b07c884323afe4e1f2da37a031.json"}}, {"family": "Del Pozo", "given": "Miguel A", "initials": "MA"}], "type": "journal article", "published": "2018-02-05", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "8", "issue": "1", "pages": "2338", "issn-l": "2045-2322"}, "abstract": "Caveolin-1 (CAV1) is over-expressed in prostate cancer (PCa) and is associated with adverse prognosis, but the molecular mechanisms linking CAV1 expression to disease progression are poorly understood. Extensive gene expression correlation analysis, quantitative multiplex imaging of clinical samples, and analysis of the CAV1-dependent transcriptome, supported that CAV1 re-programmes TGF\u03b2 signalling from tumour suppressive to oncogenic (i.e. induction of SLUG, PAI-1 and suppression of CDH1, DSP, CDKN1A). Supporting such a role, CAV1 knockdown led to growth arrest and inhibition of cell invasion in prostate cancer cell lines. Rationalized RNAi screening and high-content microscopy in search for CAV1 upstream regulators revealed integrin beta1 (ITGB1) and integrin associated proteins as CAV1 regulators. Our work suggests TGF\u03b2 signalling and beta1 integrins as potential therapeutic targets in PCa over-expressing CAV1, and contributes to better understand the paradoxical dual role of TGF\u03b2 in tumour biology.", "doi": "10.1038/s41598-018-20161-2", "pmid": "29402961", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5799174"}, {"db": "pii", "key": "10.1038/s41598-018-20161-2"}], "notes": [], "created": "2018-12-05T12:49:29.504Z", "modified": "2026-09-23T09:32:29.091Z"}, {"entity": "publication", "iuid": "fd87881f45cd4da1ba14bb416cd53804", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fd87881f45cd4da1ba14bb416cd53804.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fd87881f45cd4da1ba14bb416cd53804"}}, "title": "Systems pathology by multiplexed immunohistochemistry and whole-slide digital image analysis.", "authors": [{"family": "Blom", "given": "Sami", "initials": "S", "orcid": "0000-0002-1878-8911", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/29223b758c654de896c5ed1addf3e3a9.json"}}, {"family": "Paavolainen", "given": "Lassi", "initials": "L"}, {"family": "Bychkov", "given": "Dmitrii", "initials": "D"}, {"family": "Turkki", "given": "Riku", "initials": "R", "orcid": "0000-0002-8690-6983", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/74597043811c4f49bfe09812a9a5f3a4.json"}}, {"family": "M\u00e4ki-Teeri", "given": "Petra", "initials": "P"}, {"family": "Hemmes", "given": "Annabrita", "initials": "A"}, {"family": "V\u00e4lim\u00e4ki", "given": "Katja", "initials": "K"}, {"family": "Lundin", "given": "Johan", "initials": "J"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O", "orcid": "0000-0002-3231-0332", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb8605b07c884323afe4e1f2da37a031.json"}}, {"family": "Pellinen", "given": "Teijo", "initials": "T"}], "type": "journal article", "published": "2017-11-14", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "7", "issue": "1", "pages": "15580", "issn-l": "2045-2322"}, "abstract": "The paradigm of molecular histopathology is shifting from a single-marker immunohistochemistry towards multiplexed detection of markers to better understand the complex pathological processes. However, there are no systems allowing multiplexed IHC (mIHC) with high-resolution whole-slide tissue imaging and analysis, yet providing feasible throughput for routine use. We present an mIHC platform combining fluorescent and chromogenic staining with automated whole-slide imaging and integrated whole-slide image analysis, enabling simultaneous detection of six protein markers and nuclei, and automatic quantification and classification of hundreds of thousands of cells in situ in formalin-fixed paraffin-embedded tissues. In the first proof-of-concept, we detected immune cells at cell-level resolution (n = 128,894 cells) in human prostate cancer, and analysed T cell subpopulations in different tumour compartments (epithelium vs. stroma). In the second proof-of-concept, we demonstrated an automatic classification of epithelial cell populations (n = 83,558) and glands (benign vs. cancer) in prostate cancer with simultaneous analysis of androgen receptor (AR) and alpha-methylacyl-CoA (AMACR) expression at cell-level resolution. We conclude that the open-source combination of 8-plex mIHC detection, whole-slide image acquisition and analysis provides a robust tool allowing quantitative, spatially resolved whole-slide tissue cytometry directly in formalin-fixed human tumour tissues for improved characterization of histology and the tumour microenvironment.", "doi": "10.1038/s41598-017-15798-4", "pmid": "29138507", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5686230"}, {"db": "pii", "key": "10.1038/s41598-017-15798-4"}], "notes": [], "created": "2018-12-05T12:26:17.148Z", "modified": "2026-09-23T07:26:12.425Z"}]}