{"entity": "researcher", "timestamp": "2026-08-20T20:46:36.015Z", "family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "affiliations": ["School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden", "School of Engineering Sciences in Chemistry, Biotechnology and Health, Science for Life Laboratory, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden"], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24"}}, "publications": [{"entity": "publication", "iuid": "8cd9028a4df04b1ea0d961df50c35df0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8cd9028a4df04b1ea0d961df50c35df0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8cd9028a4df04b1ea0d961df50c35df0"}}, "title": "Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation.", "authors": [{"family": "Sporre", "given": "Emil", "initials": "E"}, {"family": "Karlsen", "given": "Jan", "initials": "J"}, {"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "Asplund-Samuelsson", "given": "Johannes", "initials": "J"}, {"family": "Janasch", "given": "Markus", "initials": "M"}, {"family": "Strandberg", "given": "Linn\u00e9a", "initials": "L"}, {"family": "Karlsson", "given": "Anna", "initials": "A"}, {"family": "Kotol", "given": "David", "initials": "D", "orcid": "0000-0002-5388-3826", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c41f8f491c474e6bba2047b5d04b4af5.json"}}, {"family": "Zeckey", "given": "Luise", "initials": "L"}, {"family": "Piazza", "given": "Ilaria", "initials": "I"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO"}, {"family": "Edfors", "given": "Fredrik", "initials": "F"}, {"family": "Hudson", "given": "Elton P", "initials": "EP", "orcid": "0000-0003-1899-7649", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3b1c894d4c8b48569b9879d0342b91dd.json"}}], "type": "journal article", "published": "2023-09-18", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "6", "issue": "1", "pages": "947", "issn-l": "2399-3642"}, "abstract": "Metabolite-level regulation of enzyme activity is important for microbes to cope with environmental shifts. Knowledge of such regulations can also guide strain engineering for biotechnology. Here we apply limited proteolysis-small molecule mapping (LiP-SMap) to identify and compare metabolite-protein interactions in the proteomes of two cyanobacteria and two lithoautotrophic bacteria that fix CO2 using the Calvin cycle. Clustering analysis of the hundreds of detected interactions shows that some metabolites interact in a species-specific manner. We estimate that approximately 35% of interacting metabolites affect enzyme activity in vitro, and the effect is often minor. Using LiP-SMap data as a guide, we find that the Calvin cycle intermediate glyceraldehyde-3-phosphate enhances activity of fructose-1,6/sedoheptulose-1,7-bisphosphatase (F/SBPase) from Synechocystis sp. PCC 6803 and Cupriavidus necator in reducing conditions, suggesting a convergent feed-forward activation of the cycle. In oxidizing conditions, glyceraldehyde-3-phosphate inhibits Synechocystis F/SBPase by promoting enzyme aggregation. In contrast, the glycolytic intermediate glucose-6-phosphate activates F/SBPase from Cupriavidus necator but not F/SBPase from Synechocystis. Thus, metabolite-level regulation of the Calvin cycle is more prevalent than previously appreciated.", "doi": "10.1038/s42003-023-05318-8", "pmid": "37723200", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10507043"}, {"db": "pii", "key": "10.1038/s42003-023-05318-8"}], "notes": [], "created": "2026-08-20T09:25:15.821Z", "modified": "2026-08-20T09:25:15.899Z"}, {"entity": "publication", "iuid": "eb7bb61312184d35a5750fd1fedc7fd0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/eb7bb61312184d35a5750fd1fedc7fd0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/eb7bb61312184d35a5750fd1fedc7fd0"}}, "title": "Design, structure and plasma binding of ancestral \u03b2-CoV scaffold antigens", "authors": [{"family": "Syren", "given": "Per Olof", "initials": "PO"}, {"family": "Hueting", "given": "David", "initials": "D"}, {"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "Zuo", "given": "Fanglei", "initials": "F", "orcid": "0000-0002-4545-1265", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aff6db3e84404d4b90e9ecfb2e6d8a77.json"}}, {"family": "Du", "given": "Likun", "initials": "L"}, {"family": "Persson", "given": "Helena", "initials": "H"}, {"family": "Hofstr\u00f6m", "given": "Camilla", "initials": "C"}, {"family": "Ohlin", "given": "Mats", "initials": "M", "orcid": "0000-0002-5105-1938", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/85422da1f8c441f7848c4850abef7555.json"}}, {"family": "Wallden", "given": "Karin", "initials": "K"}, {"family": "Hammarstrom", "given": "Lennart", "initials": "L"}, {"family": "Marcotte", "given": "Harold", "initials": "H", "orcid": "0000-0002-7168-349X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6e082f9bcfa8424987aa9e205396e5f3.json"}}, {"family": "Pan-Hammarstrom", "given": "Qiang", "initials": "Q", "orcid": "0000-0003-1990-8804", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3ba17712dd394791a8813f7643e72aaf.json"}}, {"family": "Andrell", "given": "Juni", "initials": "J"}], "type": "posted-content", "published": "2022-09-16", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-1909545/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:58:26.031Z", "modified": "2026-08-20T12:58:26.185Z"}, {"entity": "publication", "iuid": "d6315b5e655a4a468d500d6692c69532", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d6315b5e655a4a468d500d6692c69532.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d6315b5e655a4a468d500d6692c69532"}}, "title": "Conformational Selection in Biocatalytic Plastic Degradation by PETase", "authors": [{"family": "Guo", "given": "Boyang", "initials": "B", "orcid": "0000-0001-5510-1392", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be843859e5a34c66b2c914c6f2bb955f.json"}}, {"family": "Vanga", "given": "Sudarsana Reddy", "initials": "SR"}, {"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X"}, {"family": "Saenz-Mendez", "given": "Patricia", "initials": "P", "orcid": "0000-0002-6711-4972", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0efb9bc5eb7344bea1f44272fa4b2540.json"}}, {"family": "Ericsson", "given": "Sara R\u00f6nnblad", "initials": "SR"}, {"family": "Fang", "given": "Yuan", "initials": "Y"}, {"family": "Ye", "given": "Xinchen", "initials": "X"}, {"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "B\u00e4ckstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}, {"family": "Fur\u00f3", "given": "Istv\u00e1n", "initials": "I", "orcid": "0000-0002-0231-3970", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e47a4c36b86429e8b17845c02387f5d.json"}}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2022-03-18", "journal": {"title": "ACS Catal", "issn": "2155-5435", "volume": "12", "issue": "6", "pages": "3397-3409", "issn-l": "2155-5435"}, "abstract": null, "doi": "10.1021/acscatal.1c05548", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:09:01.454Z", "modified": "2026-08-20T08:09:01.681Z"}, {"entity": "publication", "iuid": "0be796669e584dfa8ea662af78c39431", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0be796669e584dfa8ea662af78c39431.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0be796669e584dfa8ea662af78c39431"}}, "title": "Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation", "authors": [{"family": "Sporre", "given": "Emil", "initials": "E", "orcid": "0000-0003-4105-7567", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2250c46520424ec69e3d736c2bf30099.json"}}, {"family": "Karlsen", "given": "Jan", "initials": "J", "orcid": "0000-0002-2821-9026", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a1c2fa8404749b0a797e90e9c6cb09e.json"}}, {"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "Samuelsson", "given": "Johannes Asplund", "initials": "JA", "orcid": "0000-0001-8077-5305", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1cf0c49cbb849b194b0b46582441d09.json"}}, {"family": "Janasch", "given": "Markus", "initials": "M", "orcid": "0000-0001-7745-720X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/115ab90674ba4090be1b3ef9a01549e4.json"}}, {"family": "Strandberg", "given": "Linn\u00e9a", "initials": "L", "orcid": "0000-0001-5386-5460", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ea4741b77234afe83e92c3dfff0a799.json"}}, {"family": "Kotol", "given": "David", "initials": "D", "orcid": "0000-0002-5388-3826", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c41f8f491c474e6bba2047b5d04b4af5.json"}}, {"family": "Zeckey", "given": "Luise", "initials": "L"}, {"family": "Piazza", "given": "Ilaria", "initials": "I", "orcid": "0000-0001-5895-6134", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5258dce396444d9a8f5e5bc2fd13b282.json"}}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}, {"family": "Edfors", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-0017-7987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9e213b04f8984c8eb00c68f5bc2f6a36.json"}}, {"family": "Hudson", "given": "Elton P", "initials": "EP", "orcid": "0000-0003-1899-7649", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3b1c894d4c8b48569b9879d0342b91dd.json"}}], "type": "posted-content", "published": "2022-03-15", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.03.15.483797", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:10:14.448Z", "modified": "2026-08-20T10:10:14.574Z"}, {"entity": "publication", "iuid": "655947c203b44a9f8a690e2cf9961d78", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/655947c203b44a9f8a690e2cf9961d78.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/655947c203b44a9f8a690e2cf9961d78"}}, "title": "Engineering of Ancestors as a Tool to Elucidate Structure, Mechanism, and Specificity of Extant Terpene Cyclase.", "authors": [{"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "Saenz-Mendez", "given": "Patricia", "initials": "P", "orcid": "0000-0002-6711-4972", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0efb9bc5eb7344bea1f44272fa4b2540.json"}}, {"family": "Rudraraju", "given": "Reshma Srilakshmi", "initials": "RS"}, {"family": "Hendrikse", "given": "Natalie M", "initials": "NM", "orcid": "0000-0001-8644-3408", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/711d4343f4594d87852869666b347f98.json"}}, {"family": "Hudson", "given": "Elton P", "initials": "EP"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Schnell", "given": "Robert", "initials": "R"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2021-03-17", "journal": {"title": "Journal of the American Chemical Society", "issn": "1520-5126", "volume": "143", "issue": "10", "pages": "3794-3807", "issn-l": "0002-7863"}, "abstract": "Structural information is crucial for understanding catalytic mechanisms and to guide enzyme engineering efforts of biocatalysts, such as terpene cyclases. However, low sequence similarity can impede homology modeling, and inherent protein instability presents challenges for structural studies. We hypothesized that X-ray crystallography of engineered thermostable ancestral enzymes can enable access to reliable homology models of extant biocatalysts. We have applied this concept in concert with molecular modeling and enzymatic assays to understand the structure activity relationship of spiroviolene synthase, a class I terpene cyclase, aiming to engineer its specificity. Engineering a surface patch in the reconstructed ancestor afforded a template structure for generation of a high-confidence homology model of the extant enzyme. On the basis of structural considerations, we designed and crystallized ancestral variants with single residue exchanges that exhibited tailored substrate specificity and preserved thermostability. We show how the two single amino acid alterations identified in the ancestral scaffold can be transferred to the extant enzyme, conferring a specificity switch that impacts the extant enzyme's specificity for formation of the diterpene spiroviolene over formation of sesquiterpenes hedycaryol and farnesol by up to 25-fold. This study emphasizes the value of ancestral sequence reconstruction combined with enzyme engineering as a versatile tool in chemical biology.", "doi": "10.1021/jacs.0c10214", "pmid": "33496585", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8023661"}], "notes": [], "created": "2026-08-20T08:45:04.766Z", "modified": "2026-08-20T08:45:04.843Z"}]}