{"entity": "researcher", "timestamp": "2026-09-29T15:21:41.962Z", "family": "Chen", "given": "Lei", "initials": "L", "orcid": "0000-0002-5226-1427", "affiliations": ["Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-752 37, Uppsala, Sweden. lei.chen@raritybioscience.com.", "Rarity Bioscience AB, SE-752 37, Uppsala, Sweden. lei.chen@raritybioscience.com."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d159fdbe9e44089c16d5a3488b0191.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d159fdbe9e44089c16d5a3488b0191"}}, "publications": [{"entity": "publication", "iuid": "fe400e6613ab41e9b97ffe67ca4240ba", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fe400e6613ab41e9b97ffe67ca4240ba.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fe400e6613ab41e9b97ffe67ca4240ba"}}, "title": "Ultra-sensitive monitoring of leukemia patients using superRCA mutation detection assays.", "authors": [{"family": "Chen", "given": "Lei", "initials": "L", "orcid": "0000-0002-5226-1427", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d159fdbe9e44089c16d5a3488b0191.json"}}, {"family": "Eriksson", "given": "Anna", "initials": "A"}, {"family": "Westr\u00f6m", "given": "Simone", "initials": "S"}, {"family": "Pandzic", "given": "Tatjana", "initials": "T"}, {"family": "Lehmann", "given": "S\u00f6ren", "initials": "S"}, {"family": "Cavelier", "given": "Lucia", "initials": "L"}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}], "type": "journal article", "published": "2022-07-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "13", "issue": "1", "pages": "4033", "issn-l": "2041-1723"}, "abstract": "Rare tumor-specific mutations in patient samples serve as excellent markers to monitor the course of malignant disease and responses to therapy in clinical routine, and improved assay techniques are needed for broad adoption. We describe herein a highly sensitive and selective molecule amplification technology - superRCA assays - for rapid and highly specific detection of DNA sequence variants present at very low frequencies in DNA samples. Using a standard flow cytometer we demonstrate precise, ultra-sensitive detection of single-nucleotide mutant sequences from malignant cells against up to a 100,000-fold excess of DNA from normal cells in either bone marrow or peripheral blood, to follow the course of patients treated for acute myeloid leukemia (AML). We also demonstrate that sequence variants located in a high-GC region may be sensitively detected, and we illustrate the potential of the technology for early detection of disease recurrence as a basis for prompt change of therapy.", "doi": "10.1038/s41467-022-31397-y", "pmid": "35821208", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9276831"}, {"db": "pii", "key": "10.1038/s41467-022-31397-y"}], "notes": [], "created": "2026-09-23T10:09:55.045Z", "modified": "2026-09-23T10:48:14.662Z"}, {"entity": "publication", "iuid": "af14c4f912b94a47b6c7e1dcb6888352", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/af14c4f912b94a47b6c7e1dcb6888352.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/af14c4f912b94a47b6c7e1dcb6888352"}}, "title": "Nanoarrays on Passivated Aluminum Surface for Site-Specific Immobilization of Biomolecules", "authors": [{"family": "Li", "given": "Shiyu", "initials": "S", "orcid": "0000-0003-4948-8353", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/599a0ea853e24a85ad5004d2d6ebcd02.json"}}, {"family": "Zeng", "given": "Shuangshuang", "initials": "S"}, {"family": "Chen", "given": "Lei", "initials": "L", "orcid": "0000-0002-5226-1427", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d159fdbe9e44089c16d5a3488b0191.json"}}, {"family": "Zhang", "given": "Zhen", "initials": "Z", "orcid": "0000-0003-4317-9701", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cdd73a7eca5646d6ae3de42605ccdf0d.json"}}, {"family": "Hjort", "given": "Klas", "initials": "K"}, {"family": "Zhang", "given": "Shi Li", "initials": "SL"}], "type": "journal-article", "published": "2018-07-16", "journal": {"title": "ACS Appl Bio Mater", "issn": "2576-6422", "volume": "1", "issue": "1", "pages": "125-135", "issn-l": null}, "abstract": null, "doi": "10.1021/acsabm.8b00037", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T13:29:40.213Z", "modified": "2026-09-23T13:29:40.283Z"}, {"entity": "publication", "iuid": "506dd221183040ff8cac95142843e7a7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/506dd221183040ff8cac95142843e7a7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/506dd221183040ff8cac95142843e7a7"}}, "title": "Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.", "authors": [{"family": "Blokzijl", "given": "Andries", "initials": "A"}, {"family": "Chen", "given": "Lei E", "initials": "LE", "orcid": "0000-0002-5226-1427", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d159fdbe9e44089c16d5a3488b0191.json"}}, {"family": "Gustafsdottir", "given": "Sigrun M", "initials": "SM"}, {"family": "Vuu", "given": "Jimmy", "initials": "J"}, {"family": "Ullenhag", "given": "Gustav", "initials": "G"}, {"family": "K\u00e4mpe", "given": "Olle", "initials": "O"}, {"family": "Landegren", "given": "Ulf", "initials": "U"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Hedstrand", "given": "H\u00e5kan", "initials": "H"}], "type": "journal article", "published": "2016-04-25", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "11", "issue": "4", "pages": "e0154214", "issn-l": "1932-6203"}, "abstract": "The diagnosis of malignant melanoma currently relies on clinical inspection of the skin surface and on the histopathological status of the excised tumor. The serum marker S100B is used for prognostic estimates at later stages of the disease, but analyses are marred by false positives and inadequate sensitivity in predicting relapsing disorder.\n\nTo investigate SOX10 as a potential biomarker for melanoma and vitiligo.\n\nIn this study we have applied proximity ligation assay (PLA) to detect the transcription factor SOX10 as a possible serum marker for melanoma. We studied a cohort of 110 melanoma patients. We further investigated a second cohort of 85 patients with vitiligo, which is a disease that also affects melanocytes.\n\nThe specificity of the SOX10 assay in serum was high, with only 1% of healthy blood donors being positive. In contrast, elevated serum SOX10 was found with high frequency among vitiligo and melanoma patients. In patients with metastases, lack of SOX10 detection was associated with treatment benefit. In two responding patients, a change from SOX10 positivity to undetectable levels was seen before the response was evident clinically.\n\nWe show for the first time that SOX10 represents a promising new serum melanoma marker for detection of early stage disease, complementing the established S100B marker. Our findings imply that SOX10 can be used to monitor responses to treatment and to assess if the treatment is of benefit at stages earlier than what is possible radiologically.", "doi": "10.1371/journal.pone.0154214", "pmid": "27110718", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC4844164"}, {"db": "pii", "key": "PONE-D-15-50680"}], "notes": [], "created": "2018-12-05T12:17:48.141Z", "modified": "2026-09-23T13:05:06.617Z"}]}