{"entity": "researcher", "timestamp": "2026-09-25T23:03:43.837Z", "family": "Wennmalm", "given": "Stefan", "initials": "S", "orcid": "0000-0002-1850-5440", "affiliations": ["Department of Applied Physics, SciLifeLab, Royal Institute of Technology, 171 65 Solna, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/274ce80e248b4866bfae98ff1ce34352.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/274ce80e248b4866bfae98ff1ce34352"}}, "publications": [{"entity": "publication", "iuid": "42dd11880c374953bafeec2b14a09d1d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/42dd11880c374953bafeec2b14a09d1d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/42dd11880c374953bafeec2b14a09d1d"}}, "title": "Detection and quantification of Na,K-ATPase dimers in the plasma membrane of living cells by FRET-FCS.", "authors": [{"family": "Nordahl", "given": "Linnea", "initials": "L"}, {"family": "Akkuratov", "given": "Evgeny E", "initials": "EE", "orcid": "0000-0002-2552-9512", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/abd9e4662bb543eebb0451af868bc319.json"}}, {"family": "Heimg\u00e4rtner", "given": "Johannes", "initials": "J", "orcid": "0000-0003-0059-9888", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ec13a513f5840648bde7466a1dba06a.json"}}, {"family": "Schach", "given": "Katja", "initials": "K"}, {"family": "Meineke", "given": "Birthe", "initials": "B", "orcid": "0000-0002-8912-0783", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3f113effede64175b4180e0fec987640.json"}}, {"family": "Els\u00e4sser", "given": "Simon", "initials": "S", "orcid": "0000-0001-8724-4849", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/faaf7145e4044091a2fed9069f56b92e.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S", "orcid": "0000-0002-1850-5440", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/274ce80e248b4866bfae98ff1ce34352.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Biochim Biophys Acta Gen Subj", "issn": "1872-8006", "volume": "1868", "issue": "7", "pages": "130619", "issn-l": "0304-4165"}, "abstract": "The sodium potassium pump, Na,K-ATPase (NKA), is an integral plasma membrane protein, expressed in all eukaryotic cells. It is responsible for maintaining the transmembrane Na+ gradient and is the major determinant of the membrane potential. Self-interaction and oligomerization of NKA in cell membranes has been proposed and discussed but is still an open question. Here, we have used a combination of FRET and Fluorescence Correlation Spectroscopy, FRET-FCS, to analyze NKA in the plasma membrane of living cells. Click chemistry was used to conjugate the fluorescent labels Alexa 488 and Alexa 647 to non-canonical amino acids introduced in the NKA \u03b11 and \u03b21 subunits. We demonstrate that FRET-FCS can detect an order of magnitude lower concentration of green-red labeled protein pairs in a single-labeled red and green background than what is possible with cross-correlation (FCCS). We show that a significant fraction of NKA is expressed as a dimer in the plasma membrane. We also introduce a method to estimate not only the number of single and double labeled NKA, but the number of unlabeled, endogenous NKA and estimate the density of endogenous NKA at the plasma membrane to 1400 \u00b1 800 enzymes/\u03bcm2.", "doi": "10.1016/j.bbagen.2024.130619", "pmid": "38643888", "labels": {"Simon Els\u00e4sser": "", "SciLifeLab Fellow": ""}, "xrefs": [{"db": "pii", "key": "S0304-4165(24)00062-X"}], "notes": [], "created": "2024-11-25T23:09:33.346Z", "modified": "2026-09-23T14:48:16.589Z"}, {"entity": "publication", "iuid": "910e4dc936754e4083a07c236c0c25a4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/910e4dc936754e4083a07c236c0c25a4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/910e4dc936754e4083a07c236c0c25a4"}}, "title": "Biodistribution of DNA-origami nanostructures in live zebrafish embryos with single-cell resolution", "authors": [{"family": "Kolonelou", "given": "Christina", "initials": "C", "orcid": "0000-0002-5625-5569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/48cfd6b4505d4325a934fbe7d35d1875.json"}}, {"family": "Br\u00e4utigam", "given": "Lars", "initials": "L", "orcid": "0000-0003-2834-1512", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/91e3c53b11534903aee0e435a86eec3e.json"}}, {"family": "Edwards", "given": "Steven", "initials": "S"}, {"family": "Engstr\u00f6m", "given": "Enya", "initials": "E", "orcid": "0009-0009-6574-0652", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a3762eb3425447e8c0805a9bb0059a0.json"}}, {"family": "Dias", "given": "Jos\u00e9 M", "initials": "JM", "orcid": "0000-0002-1402-0323", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/baa189d497af4380b7db4c1aba55ae80.json"}}, {"family": "Spratt", "given": "Joel", "initials": "J", "orcid": "0000-0002-8720-5011", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/49c3eb2348194851aa20cbeb4e1c3264.json"}}, {"family": "Karampelias", "given": "Christos", "initials": "C", "orcid": "0000-0002-9990-5760", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/eb41842e9b36439cbcbf097378a82378.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S", "orcid": "0000-0002-1850-5440", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/274ce80e248b4866bfae98ff1ce34352.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "Andersson", "given": "Olov", "initials": "O", "orcid": "0000-0001-6715-781X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2f39763e006e48309fc287cdb644ffe4.json"}}, {"family": "Teixeira", "given": "Ana I", "initials": "AI", "orcid": "0000-0001-8169-8815", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1e6497a9de14f5b901fbca4a223ddad.json"}}], "type": "posted-content", "published": "2023-12-23", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2023.12.22.572973", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T14:36:27.423Z", "modified": "2026-09-23T14:49:29.755Z"}, {"entity": "publication", "iuid": "649894a9bab14c5d9cd0c851ed3170a1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/649894a9bab14c5d9cd0c851ed3170a1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/649894a9bab14c5d9cd0c851ed3170a1"}}, "title": "\u03b1-Synuclein-induced deformation of small unilamellar vesicles.", "authors": [{"family": "Makasewicz", "given": "Katarzyna", "initials": "K", "orcid": "0000-0001-7224-5750", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/633bf8178b8b4afd9523466931f4f1d7.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S", "orcid": "0000-0002-1850-5440", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/274ce80e248b4866bfae98ff1ce34352.json"}}, {"family": "Linse", "given": "Sara", "initials": "S", "orcid": "0000-0001-9629-7109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed0f1aa3c40c44b687cb4be5082f0966.json"}}, {"family": "Sparr", "given": "Emma", "initials": "E", "orcid": "0000-0001-8343-9657", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a692e20cc68741be9221c981481f2a4d.json"}}], "type": "journal article", "published": "2022-07-25", "journal": {"title": "QRB Discov", "issn": "2633-2892", "volume": "3", "pages": "e10", "issn-l": null}, "abstract": "\u03b1-Synuclein is a small neuronal protein that reversibly associates with lipid membranes. The membrane interactions are believed to be central to the healthy function of this protein involved in synaptic plasticity and neurotransmitter release. \u03b1-Synuclein has been speculated to induce vesicle fusion as well as fission, processes which are analogous to each other but proceed in different directions and involve different driving forces. In the current work, we analyse \u03b1-synuclein-induced small unilamellar vesicle deformation from a thermodynamics point of view. We show that the structures interpreted in the literature as fusion intermediates are in fact a stable deformed state and neither fusion nor vesicle clustering occurs. We speculate on the driving force for the observed deformation and put forward a hypothesis that \u03b1-synuclein self-assembly on the lipid membrane precedes and induces membrane remodelling.", "doi": "10.1017/qrd.2022.9", "pmid": "37529290", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10392696"}, {"db": "pii", "key": "S2633289222000096"}], "notes": [], "created": "2026-09-23T12:08:09.096Z", "modified": "2026-09-23T12:08:09.246Z"}]}