{"entity": "researcher", "timestamp": "2026-08-20T21:13:14.395Z", "family": "Diederich", "given": "Benedict", "initials": "B", "orcid": "0000-0003-0453-6286", "affiliations": ["H. Wang, B. Diederich, Leibniz Institute for Photonic Technology, Albert-Einstein-Str. 9, 07749, Jena, Germany.", "H. Wang, B. Diederich, Institute of Physical Chemistry, Friedrich-Schiller-Universit\u00e4t Jena, Helmholtzweg 4, 07743, Jena, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/25fb1523d3854ce59fdfe3841250ac3c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/25fb1523d3854ce59fdfe3841250ac3c"}}, "publications": [{"entity": "publication", "iuid": "983f5cce99e346089c9358266a353b6c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/983f5cce99e346089c9358266a353b6c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/983f5cce99e346089c9358266a353b6c"}}, "title": "ESPressoscope: A small and powerful approach for in situ microscopy.", "authors": [{"family": "Li", "given": "Ethan", "initials": "E", "orcid": "0000-0002-3041-0428", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d18c3583619e4e5e8d2f7a4596ff557a.json"}}, {"family": "Saggiomo", "given": "Vittorio", "initials": "V"}, {"family": "Ouyang", "given": "Wei", "initials": "W"}, {"family": "Prakash", "given": "Manu", "initials": "M", "orcid": "0000-0002-8046-8388", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ffb31ee2e27483b9303ce73a9e6f3d1.json"}}, {"family": "Diederich", "given": "Benedict", "initials": "B", "orcid": "0000-0003-0453-6286", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/25fb1523d3854ce59fdfe3841250ac3c.json"}}], "type": "journal article", "published": "2024-10-16", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "19", "issue": "10", "pages": "e0306654", "issn-l": "1932-6203"}, "abstract": "Microscopy is essential for detecting, identifying, analyzing, and measuring small objects. Access to modern microscopy equipment is crucial for scientific research, especially in the biomedical and analytical sciences. However, the high cost of equipment, limited availability of parts, and challenges associated with transporting equipment often limit the accessibility and operational capabilities of these tools, particularly in field sites and other remote or resource-limited settings. Thus, there is a need for affordable and accessible alternatives to traditional microscopy systems. We address this challenge by investigating the feasibility of using a simple microcontroller board not only as a portable and field-ready digital microscope, but furthermore as a versatile platform which can easily be adapted to a variety of imaging applications. By adding a few external components, we demonstrate that a low-cost ESP32 camera board can be used to build an autonomous in situ platform for digital time-lapse imaging of cells. Our prototype of this approach, which we call ESPressoscope, can be adapted to applications ranging from monitoring incubator cell cultures in the lab to observing ecological phenomena in the sea, and it can be adapted for other techniques such as microfluidics or spectrophotometry. Our prototype of the ESPressoscope concept achieves a low power consumption and small size, which makes it ideal for field research in environments and applications where microscopy was previously infeasible. Its Wi-Fi connectivity enables integration with external image processing and storage systems, including on cloud platforms when internet access is available. Finally, we present several web browser-based tools to help users operate and manage our prototype's software. Our findings demonstrate the potential for low-cost, portable microscopy solutions to enable new and more accessible experiments for biological and analytical applications.", "doi": "10.1371/journal.pone.0306654", "pmid": "39413076", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11482665"}, {"db": "pii", "key": "PONE-D-24-07702"}], "notes": [], "created": "2026-08-20T12:44:30.457Z", "modified": "2026-08-20T12:44:30.584Z"}, {"entity": "publication", "iuid": "12b7c1d2703f4e8680f8d1249e0bb9ad", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/12b7c1d2703f4e8680f8d1249e0bb9ad.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/12b7c1d2703f4e8680f8d1249e0bb9ad"}}, "title": "ESPressoscope: a small and powerful platform for in situ microscopy", "authors": [{"family": "Li", "given": "Ethan", "initials": "E"}, {"family": "Saggiomo", "given": "Vittorio", "initials": "V"}, {"family": "Ouyang", "given": "Wei", "initials": "W"}, {"family": "Prakash", "given": "Manu", "initials": "M", "orcid": "0000-0002-8046-8388", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ffb31ee2e27483b9303ce73a9e6f3d1.json"}}, {"family": "Diederich", "given": "Benedict", "initials": "B", "orcid": "0000-0003-0453-6286", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/25fb1523d3854ce59fdfe3841250ac3c.json"}}], "type": "posted-content", "published": "2024-01-31", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.01.20.576174", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:51:33.166Z", "modified": "2026-08-20T10:51:33.268Z"}, {"entity": "publication", "iuid": "6d5151bbccf94e4887c6eb2f698be76a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6d5151bbccf94e4887c6eb2f698be76a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6d5151bbccf94e4887c6eb2f698be76a"}}, "title": "An Open-Source Modular Framework for Automated Pipetting and Imaging Applications.", "authors": [{"family": "Ouyang", "given": "Wei", "initials": "W"}, {"family": "Bowman", "given": "Richard W", "initials": "RW"}, {"family": "Wang", "given": "Haoran", "initials": "H"}, {"family": "Bumke", "given": "Kaspar E", "initials": "KE"}, {"family": "Collins", "given": "Joel T", "initials": "JT"}, {"family": "Spjuth", "given": "Ola", "initials": "O"}, {"family": "Carreras-Puigvert", "given": "Jordi", "initials": "J"}, {"family": "Diederich", "given": "Benedict", "initials": "B", "orcid": "0000-0003-0453-6286", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/25fb1523d3854ce59fdfe3841250ac3c.json"}}], "type": "journal article", "published": "2022-04-00", "journal": {"title": "Adv Biol (Weinh)", "issn": "2701-0198", "volume": "6", "issue": "4", "pages": "e2101063", "issn-l": null}, "abstract": "The number of samples in biological experiments is continuously increasing, but complex protocols and human error in many cases lead to suboptimal data quality and hence difficulties in reproducing scientific findings. Laboratory automation can alleviate many of these problems by precisely reproducing machine-readable protocols. These instruments generally require high up-front investments, and due to the lack of open application programming interfaces (APIs), they are notoriously difficult for scientists to customize and control outside of the vendor-supplied software. Here, automated, high-throughput experiments are demonstrated for interdisciplinary research in life science that can be replicated on a modest budget, using open tools to ensure reproducibility by combining the tools OpenFlexure, Opentrons, ImJoy, and UC2. This automated sample preparation and imaging pipeline can easily be replicated and established in many laboratories as well as in educational contexts through easy-to-understand algorithms and easy-to-build microscopes. Additionally, the creation of feedback loops, with later pipetting or imaging steps depending on the analysis of previously acquired images, enables the realization of fully autonomous \"smart\" microscopy experiments. All documents and source files are publicly available to prove the concept of smart lab automation using inexpensive, open tools. It is believed this democratizes access to the power and repeatability of automated experiments.", "doi": "10.1002/adbi.202101063", "pmid": "34693668", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:27:21.416Z", "modified": "2026-08-20T06:27:21.537Z"}, {"entity": "publication", "iuid": "849012b13e6d40bebf1e5edd27cc7d24", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/849012b13e6d40bebf1e5edd27cc7d24.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/849012b13e6d40bebf1e5edd27cc7d24"}}, "title": "An Open-Source Modular Framework for Automated Pipetting and Imaging Applications", "authors": [{"family": "Ouyang", "given": "Wei", "initials": "W"}, {"family": "Bowman", "given": "Richard", "initials": "R", "orcid": "0000-0002-1531-8199", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dd8285f431ce4ff59d9b6b288cec6b9d.json"}}, {"family": "Wang", "given": "Haoran", "initials": "H"}, {"family": "Bumke", "given": "Kaspar E", "initials": "KE"}, {"family": "Collins", "given": "Joel T", "initials": "JT"}, {"family": "Spjuth", "given": "Ola", "initials": "O", "orcid": "0000-0002-8083-2864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c192389f99d4801b91f3350e07dfb9e.json"}}, {"family": "Carreras-Puigvert", "given": "Jordi", "initials": "J", "orcid": "0000-0002-7671-3707", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a9c300823ae241eea419a01e4397fcc2.json"}}, {"family": "Diederich", "given": "Benedict", "initials": "B", "orcid": "0000-0003-0453-6286", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/25fb1523d3854ce59fdfe3841250ac3c.json"}}], "type": "posted-content", "published": "2021-06-24", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2021.06.24.449732", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:05:13.473Z", "modified": "2026-08-20T10:05:13.584Z"}]}