{"entity": "researcher", "timestamp": "2026-05-09T16:41:00.376Z", "family": "Sarlus", "given": "Heela", "initials": "H", "orcid": "0000-0001-7880-9828", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/2458d82ce194489a9ef5719882dfba0c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/2458d82ce194489a9ef5719882dfba0c"}}, "publications": [{"entity": "publication", "iuid": "7a0120e1ffd2468991bd9de55ea66308", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7a0120e1ffd2468991bd9de55ea66308.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7a0120e1ffd2468991bd9de55ea66308"}}, "title": "Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation.", "authors": [{"family": "Zhu", "given": "Keying", "initials": "K", "orcid": "0000-0001-7500-1532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a3555d961bb34bedae569a2b05ffc474.json"}}, {"family": "Wang", "given": "Yang", "initials": "Y", "orcid": "0000-0002-7911-9551", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b6a9aa7f9d8749b3843f4e7730e12840.json"}}, {"family": "Sarlus", "given": "Heela", "initials": "H", "orcid": "0000-0001-7880-9828", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2458d82ce194489a9ef5719882dfba0c.json"}}, {"family": "Geng", "given": "Keyi", "initials": "K", "orcid": "0000-0003-0892-7460", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a56ac387ab324409b2c0dc24b9be5d73.json"}}, {"family": "Nutma", "given": "Erik", "initials": "E"}, {"family": "Sun", "given": "Jingxian", "initials": "J"}, {"family": "Kung", "given": "Shin-Yu", "initials": "SY", "orcid": "0000-0003-4002-2848", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ffccfc627579496ea4baf20afa00116e.json"}}, {"family": "Bay", "given": "Cindy", "initials": "C", "orcid": "0000-0003-3898-3148", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/337c271c08004081903d2308c5bf3c8d.json"}}, {"family": "Han", "given": "Jinming", "initials": "J", "orcid": "0000-0002-6084-3275", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a33af0dcff9543d8aaf6c599814ca52c.json"}}, {"family": "Min", "given": "Jin-Hong", "initials": "JH"}, {"family": "Benito-Cuesta", "given": "Irene", "initials": "I"}, {"family": "Lund", "given": "Harald", "initials": "H", "orcid": "0000-0001-8046-0805", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/447fceec6f074e869ffb736d5dab13c1.json"}}, {"family": "Amor", "given": "Sandra", "initials": "S", "orcid": "0000-0001-6169-9845", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c8523f177f5d4b2ca288b1deda7d5beb.json"}}, {"family": "Wang", "given": "Jun", "initials": "J", "orcid": "0000-0002-4530-0758", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d877a3366dca4550831e463f83d9ad02.json"}}, {"family": "Zhang", "given": "Xing-Mei", "initials": "XM", "orcid": "0000-0002-8728-8849", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65a68d96961247bb8bb9890b20fa2305.json"}}, {"family": "Kutter", "given": "Claudia", "initials": "C", "orcid": "0000-0002-8047-0058", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a4c9dcde56304ab2a99bb1032e4ce834.json"}}, {"family": "Guerreiro-Cacais", "given": "Andr\u00e9 Ortlieb", "initials": "AO", "orcid": "0000-0002-4561-2823", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8c2ecf483cb7442b9de5c99dcc11c1a5.json"}}, {"family": "H\u00f6gberg", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0003-2715-7887", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bc6147217ff7477db7d6c14847da3d58.json"}}, {"family": "Harris", "given": "Robert A", "initials": "RA", "orcid": "0000-0003-4990-509X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7558fb5ceba54b689244b4e771714b76.json"}}], "type": "journal article", "published": "2022-07-05", "journal": {"title": "EMBO Rep.", "issn": "1469-3178", "volume": "23", "issue": "7", "pages": "e54499", "issn-l": "1469-221X"}, "abstract": "Targeting myeloid cells, especially microglia, for the treatment of neuroinflammatory diseases such as multiple sclerosis (MS), is underappreciated. Our in silico drug screening reveals topoisomerase 1 (TOP1) inhibitors as promising drug candidates for microglial modulation. We show that TOP1 is highly expressed in neuroinflammatory conditions, and TOP1 inhibition using camptothecin (CPT) and its FDA-approved analog topotecan (TPT) reduces inflammatory responses in microglia/macrophages and ameliorates neuroinflammation in vivo. Transcriptomic analyses of sorted microglia from LPS-challenged mice reveal an altered transcriptional phenotype following TPT treatment. To target myeloid cells, we design a nanosystem using \u03b2-glucan-coated DNA origami (MyloGami) loaded with TPT (TopoGami). MyloGami shows enhanced specificity to myeloid cells while preventing the degradation of the DNA origami scaffold. Myeloid-specific TOP1 inhibition using TopoGami significantly suppresses the inflammatory response in microglia and mitigates MS-like disease progression. Our findings suggest that TOP1 inhibition in myeloid cells represents a therapeutic strategy for neuroinflammatory diseases and that the myeloid-specific nanosystems we designed may also benefit the treatment of other diseases with dysfunctional myeloid cells.", "doi": "10.15252/embr.202154499", "pmid": "35593064", "labels": {"SciLifeLab Fellow": null, "Claudia Kutter": null}, "xrefs": [{"db": "pmc", "key": "PMC9253741"}], "notes": [], "created": "2022-11-28T11:21:24.134Z", "modified": "2022-11-28T11:21:24.513Z"}]}