{"entity": "researcher", "timestamp": "2026-08-23T22:11:17.847Z", "family": "Roa", "given": "Juan C", "initials": "JC", "orcid": "0000-0001-8313-8774", "affiliations": ["Departamento de Patolog\u00eda, Facultad de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/16e1c0a2257d4cf099321e98dacbb104.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/16e1c0a2257d4cf099321e98dacbb104"}}, "publications": [{"entity": "publication", "iuid": "0649c680280047fab034f8d8c1fe7464", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0649c680280047fab034f8d8c1fe7464.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0649c680280047fab034f8d8c1fe7464"}}, "title": "Evaluation of the chemopreventive potentials of ezetimibe and aspirin in a novel mouse model of gallbladder preneoplasia.", "authors": [{"family": "Rosa", "given": "Lorena", "initials": "L", "orcid": "0000-0003-3785-2146", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06b88862d6ea4d4492a0066f28ff99ae.json"}}, {"family": "Lobos-Gonz\u00e1lez", "given": "Lorena", "initials": "L", "orcid": "0000-0002-4957-9864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d84cd039171f4d5ab1047f50b93caa6f.json"}}, {"family": "Mu\u00f1oz-Durango", "given": "Natalia", "initials": "N", "orcid": "0000-0002-4435-9168", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c0bc1ef6466a42718084661efc844393.json"}}, {"family": "Garc\u00eda", "given": "Patricia", "initials": "P", "orcid": "0000-0002-3817-4896", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5a6a516d6d80444ba5fe7170585fc06a.json"}}, {"family": "Bizama", "given": "Carolina", "initials": "C", "orcid": "0000-0002-5379-6191", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c617c5dce3b43ac9647e2e359dfcc17.json"}}, {"family": "G\u00f3mez", "given": "Natalia", "initials": "N", "orcid": "0000-0001-5686-5909", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e87ba2a0c3d443fb8c1ee6c6175b6e23.json"}}, {"family": "Gonz\u00e1lez", "given": "Ximena", "initials": "X", "orcid": "0000-0002-9191-0637", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0fce78ec75524f5992ab7be409c614dd.json"}}, {"family": "Wichmann", "given": "Ignacio A", "initials": "IA", "orcid": "0000-0002-1115-2937", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bd9d5f024ba14073a8a3dc587bc26749.json"}}, {"family": "Saavedra", "given": "Nicol\u00e1s", "initials": "N", "orcid": "0000-0002-3334-5107", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6833ac7e8fb343b2ba8323cf39903a4c.json"}}, {"family": "Guevara", "given": "Francisca", "initials": "F", "orcid": "0000-0002-8907-8773", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3f855ebb33734e95855d0cabe69f90cc.json"}}, {"family": "Villegas", "given": "Jaime", "initials": "J", "orcid": "0000-0002-6367-2926", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e0b3f8dcba34abfa1c88b00b5dda286.json"}}, {"family": "Arrese", "given": "Marco", "initials": "M", "orcid": "0000-0002-0499-4191", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/efa018cdf01e49d5ab5861aafad25fbd.json"}}, {"family": "Ferreccio", "given": "Catterina", "initials": "C", "orcid": "0000-0001-6331-5534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cc91868735f741b4b6f6dc5a78a03757.json"}}, {"family": "Kalergis", "given": "Alexis M", "initials": "AM", "orcid": "0000-0001-7622-5263", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1df0fbd3388f49db8b5d32e24e90c724.json"}}, {"family": "Miquel", "given": "Juan Francisco", "initials": "JF", "orcid": "0000-0002-7849-3883", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f2f21febfe7040e7ba10e97274d5026f.json"}}, {"family": "Espinoza", "given": "Jaime A", "initials": "JA", "orcid": "0000-0002-0731-2715", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e447879a9c9945849259b6c424c75b5c.json"}}, {"family": "Roa", "given": "Juan C", "initials": "JC", "orcid": "0000-0001-8313-8774", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/16e1c0a2257d4cf099321e98dacbb104.json"}}], "type": "journal article", "published": "2020-11-00", "journal": {"title": "Mol Oncol", "issn": "1878-0261", "volume": "14", "issue": "11", "pages": "2834-2852", "issn-l": "1574-7891"}, "abstract": "Gallbladder stones (cholecystolithiasis) are the main risk factor for gallbladder cancer (GBC), a lethal biliary malignancy with poor survival rates worldwide. Gallbladder stones are thought to damage the gallbladder epithelium and trigger chronic inflammation. Preneoplastic lesions that arise in such an inflammatory microenvironment can eventually develop into invasive carcinoma, through mechanisms that are not fully understood. Here, we developed a novel gallbladder preneoplasia mouse model through the administration of two lithogenic diets (a low- or a high-cholesterol diet) in wild-type C57BL/6 mice over a period of 9 months. Additionally, we evaluated the chemopreventive potentials of the anti-inflammatory drug aspirin and the cholesterol absorption inhibitor ezetimibe. Both lithogenic diets induced early formation of gallbladder stones, together with extensive inflammatory changes and widespread induction of metaplasia, an epithelial adaptation to tissue injury. Dysplastic lesions were presented only in mice fed with high-cholesterol diet (62.5%) in late stages (9th month), and no invasive carcinoma was observed at any stage. The cholesterol absorption inhibitor ezetimibe inhibited gallbladder stone formation and completely prevented the onset of metaplasia and dysplasia in both lithogenic diets, whereas aspirin partially reduced metaplasia development only in the low-cholesterol diet setting. This model recapitulates several of the structural and inflammatory findings observed in human cholecystolithiasic gallbladders, making it relevant for the study of gallbladder carcinogenesis. In addition, our results suggest that the use of cholesterol absorption inhibitors and anti-inflammatory drugs can be evaluated as chemopreventive strategies to reduce the burden of GBC among high-risk populations.", "doi": "10.1002/1878-0261.12766", "pmid": "33326125", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7607176"}], "notes": [], "created": "2026-08-21T10:58:03.190Z", "modified": "2026-08-21T10:58:04.235Z"}, {"entity": "publication", "iuid": "1dd2061988e94d4b98cbc1fbe7ad1120", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1dd2061988e94d4b98cbc1fbe7ad1120.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1dd2061988e94d4b98cbc1fbe7ad1120"}}, "title": "Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.", "authors": [{"family": "Garc\u00eda", "given": "Patricia", "initials": "P"}, {"family": "Bizama", "given": "Carolina", "initials": "C"}, {"family": "Rosa", "given": "Lorena", "initials": "L"}, {"family": "Espinoza", "given": "Jaime A", "initials": "JA"}, {"family": "Weber", "given": "Helga", "initials": "H"}, {"family": "Cerda-Infante", "given": "Javier", "initials": "J"}, {"family": "S\u00e1nchez", "given": "Marianela", "initials": "M"}, {"family": "Montecinos", "given": "Viviana P", "initials": "VP"}, {"family": "Lorenzo-Bermejo", "given": "Justo", "initials": "J"}, {"family": "Boekstegers", "given": "Felix", "initials": "F"}, {"family": "D\u00e1vila-L\u00f3pez", "given": "Marcela", "initials": "M"}, {"family": "Alfaro", "given": "Francisca", "initials": "F"}, {"family": "Leiva-Acevedo", "given": "Claudia", "initials": "C"}, {"family": "Parra", "given": "Zasha", "initials": "Z"}, {"family": "Romero", "given": "Diego", "initials": "D"}, {"family": "Kato", "given": "Sumie", "initials": "S"}, {"family": "Leal", "given": "Pamela", "initials": "P"}, {"family": "Lagos", "given": "Marcela", "initials": "M"}, {"family": "Roa", "given": "Juan Carlos", "initials": "JC", "orcid": "0000-0001-8313-8774", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/16e1c0a2257d4cf099321e98dacbb104.json"}}], "type": "journal article", "published": "2020-04-15", "journal": {"title": "Biol Res", "issn": "0717-6287", "volume": "53", "issue": "1", "pages": "13", "issn-l": null}, "abstract": "Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry.\n\nAfter immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice.\n\nThe first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.", "doi": "10.1186/s40659-020-00282-7", "pmid": "32293552", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7158131"}, {"db": "pii", "key": "10.1186/s40659-020-00282-7"}], "notes": [], "created": "2026-08-21T12:41:42.689Z", "modified": "2026-08-21T12:41:42.767Z"}, {"entity": "publication", "iuid": "5e34a795b4cc4fc691fc29217d5460ec", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5e34a795b4cc4fc691fc29217d5460ec.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5e34a795b4cc4fc691fc29217d5460ec"}}, "title": "Mucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinoma.", "authors": [{"family": "Espinoza", "given": "Jaime A", "initials": "JA", "orcid": "0000-0002-0731-2715", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e447879a9c9945849259b6c424c75b5c.json"}}, {"family": "Riquelme", "given": "Ismael", "initials": "I"}, {"family": "Sagredo", "given": "Eduardo A", "initials": "EA"}, {"family": "Rosa", "given": "Lorena", "initials": "L"}, {"family": "Garc\u00eda", "given": "Patricia", "initials": "P"}, {"family": "Bizama", "given": "Carolina", "initials": "C"}, {"family": "Apud-Bell", "given": "Mar\u00eda", "initials": "M"}, {"family": "Leal", "given": "Pamela", "initials": "P"}, {"family": "Weber", "given": "Helga", "initials": "H"}, {"family": "Benavente", "given": "Felipe", "initials": "F"}, {"family": "Vargas", "given": "Sergio", "initials": "S"}, {"family": "Romero", "given": "Diego", "initials": "D"}, {"family": "Kalergis", "given": "Alexis M", "initials": "AM"}, {"family": "Roa", "given": "Juan Carlos", "initials": "JC", "orcid": "0000-0001-8313-8774", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/16e1c0a2257d4cf099321e98dacbb104.json"}}], "type": "journal article", "published": "2019-03-00", "journal": {"title": "Histopathology", "issn": "1365-2559", "volume": "74", "issue": "4", "pages": "597-607", "issn-l": "0309-0167"}, "abstract": "Gallbladder cancer (GBC) is an aggressive tumour that is usually diagnosed at advanced stages and is characterised by a poor prognosis. Using public data of normal human tissues, we found that mRNA and protein levels of mucin 5B (MUC5B) and carbonic anhydrase 9 (CA9) were highly increased in gallbladder tissues. In addition, previous evidence has shown that claudin 18 (CLDN18) protein expression is higher in GBC. The aim of this study was to perform an analysis of these cell surface proteins during the histological progression of GBC in order to identify their theranostic potential.\n\nMUC5B expression, CA9 expression and CLDN18 expression were examined by immunohistochemistry in a series of 179 chronic cholecystitis (including 16 metaplastic tissues), 15 dysplasia and 217 GBC samples by the use of tissue microarray analysis. A composite staining score was calculated from staining intensity and percentage of positive cells. Immunohistochemical analysis showed high expression of MUC5B and CA9 among normal epithelium, metaplastic tissues, and dysplastic tissues. However, expression of both proteins was observed in roughly 50% of GBC samples. In contrast, CLDN18 was absent in normal epithelium, but its expression was higher in metaplastic cells. Among GBC cases, approximately half showed high CLDN18 expression. No associations were found between MUC5B, CA9 and CLDN18 expression and any clinicopathological features.\n\nCLDN18 is a new metaplasia marker in gallbladder tissues, and is conserved in approximately half of GBC cases. MUC5B and CA9 are highly conserved during GBC histological progression. The three markers are potential theranostic markers, in particular CA9 and CLDN18, for which there are already targeted therapies available.", "doi": "10.1111/his.13797", "pmid": "30565710", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T12:19:06.579Z", "modified": "2026-08-21T12:19:06.646Z"}]}