{"entity": "researcher", "timestamp": "2026-09-25T23:40:49.341Z", "family": "Sekine", "given": "Keisuke", "initials": "K", "orcid": "0000-0001-5133-0876", "affiliations": ["Laboratory of Cancer Cell Systems, National Cancer Center Research Institute, Tokyo, Japan."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/148e0b25886344eb99fa630abf4bccec.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/148e0b25886344eb99fa630abf4bccec"}}, "publications": [{"entity": "publication", "iuid": "b057a6ae300c45eea830fb5f0130eda9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b057a6ae300c45eea830fb5f0130eda9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b057a6ae300c45eea830fb5f0130eda9"}}, "title": "descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.", "authors": [{"family": "Otomo", "given": "Kohei", "initials": "K", "orcid": "0000-0002-5322-6295", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/75d661ed6f40434f93aed18453fddc0e.json"}}, {"family": "Omura", "given": "Takaki", "initials": "T"}, {"family": "Nozawa", "given": "Yuki", "initials": "Y", "orcid": "0009-0002-4571-6232", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7c483e4ddcdb4c909d82398aaa8ba4a7.json"}}, {"family": "Edwards", "given": "Steven J", "initials": "SJ"}, {"family": "Sato", "given": "Yukihiko", "initials": "Y"}, {"family": "Saito", "given": "Yuri", "initials": "Y"}, {"family": "Yagishita", "given": "Shigehiro", "initials": "S"}, {"family": "Uchida", "given": "Hitoshi", "initials": "H", "orcid": "0000-0002-8952-2061", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7bae37031b1046d09e85a49387d99fc6.json"}}, {"family": "Watakabe", "given": "Yuki", "initials": "Y", "orcid": "0000-0002-6580-1007", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2007e828ad443f083dac5928b81e511.json"}}, {"family": "Naitou", "given": "Kiyotada", "initials": "K", "orcid": "0000-0001-8599-2528", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/eba850ef95394fe49092375c51f3fc38.json"}}, {"family": "Yanai", "given": "Rin", "initials": "R"}, {"family": "Sahara", "given": "Naruhiko", "initials": "N"}, {"family": "Takagi", "given": "Satoshi", "initials": "S", "orcid": "0000-0001-9749-1034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/72d01a191e1d495d9690efdebc2c02b6.json"}}, {"family": "Katayama", "given": "Ryohei", "initials": "R", "orcid": "0000-0001-7394-895X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/88b039c25e0b4338951d8a1650b79a75.json"}}, {"family": "Iwata", "given": "Yusuke", "initials": "Y", "orcid": "0009-0009-7203-772X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/08f5a40615a94f2eb4f8da4af3092fbe.json"}}, {"family": "Shiokawa", "given": "Toshiro", "initials": "T"}, {"family": "Hayakawa", "given": "Yoku", "initials": "Y"}, {"family": "Otsuka", "given": "Kensuke", "initials": "K"}, {"family": "Watanabe-Takano", "given": "Haruko", "initials": "H", "orcid": "0000-0002-2986-3702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e37e945e070b4223b05739c59cb90384.json"}}, {"family": "Haneda", "given": "Yuka", "initials": "Y", "orcid": "0000-0002-6178-1109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a532e85dcc8c49b3950014e77622ba0b.json"}}, {"family": "Fukuhara", "given": "Shigetomo", "initials": "S", "orcid": "0000-0003-2777-501X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b4907bab62941a99f5b3ba2b3158cdb.json"}}, {"family": "Fujiwara", "given": "Miku", "initials": "M"}, {"family": "Nii", "given": "Takenobu", "initials": "T"}, {"family": "Meno", "given": "Chikara", "initials": "C", "orcid": "0000-0002-0869-6642", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6bf7ca17a3b642b7afed4d9899cc2237.json"}}, {"family": "Takeshita", "given": "Naoki", "initials": "N", "orcid": "0000-0002-1418-9188", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b203f2192a314f779ec5fe68fb92d706.json"}}, {"family": "Yashiro", "given": "Kenta", "initials": "K"}, {"family": "Rosales Rocabado", "given": "Juan Marcelo", "initials": "JM", "orcid": "0000-0002-8753-2979", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a69ec26ebfbc4c20a94237be37b4899e.json"}}, {"family": "Kaku", "given": "Masaru", "initials": "M", "orcid": "0000-0002-7988-6435", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8471572576c543bb90ed18ea4dfe5558.json"}}, {"family": "Yamada", "given": "Tatsuya", "initials": "T", "orcid": "0000-0002-6906-9861", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9374b1ebb2fa4b468d0709cbbcfe3197.json"}}, {"family": "Oishi", "given": "Yumiko", "initials": "Y"}, {"family": "Koike", "given": "Hiroyuki", "initials": "H", "orcid": "0000-0001-9934-6930", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed4ab3f56ef641c7a03ad8e60631fc6b.json"}}, {"family": "Cheng", "given": "Yinglan", "initials": "Y"}, {"family": "Sekine", "given": "Keisuke", "initials": "K", "orcid": "0000-0001-5133-0876", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/148e0b25886344eb99fa630abf4bccec.json"}}, {"family": "Koga", "given": "Jun-Ichiro", "initials": "JI", "orcid": "0000-0002-5501-2477", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f410a699306041898ebafb77285a69f3.json"}}, {"family": "Sugiyama", "given": "Kaori", "initials": "K"}, {"family": "Kimura", "given": "Kenichi", "initials": "K", "orcid": "0000-0002-0363-8865", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/44cf2d4acdcb4551befb9d545e8e023d.json"}}, {"family": "Karube", "given": "Fuyuki", "initials": "F"}, {"family": "Kim", "given": "Hyeree", "initials": "H", "orcid": "0009-0007-3174-2989", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9cf871ed6964310bf818eb2a0bae058.json"}}, {"family": "Manabe", "given": "Ichiro", "initials": "I", "orcid": "0000-0001-9481-6673", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a454cd6df5cb46a19462d3b6a4e94f86.json"}}, {"family": "Nemoto", "given": "Tomomi", "initials": "T", "orcid": "0000-0001-6102-1495", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c0559bdf14b477f8af8db4647d25ed4.json"}}, {"family": "Tainaka", "given": "Kazuki", "initials": "K", "orcid": "0000-0002-5943-8723", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1bb61a31726d46f48f511316c5db3f74.json"}}, {"family": "Hamada", "given": "Akinobu", "initials": "A"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "Susaki", "given": "Etsuo A", "initials": "EA", "orcid": "0000-0001-5621-7311", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/03b5ddba5f5c4a08be718ebb47ef2b32.json"}}], "type": "journal article", "published": "2024-06-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "4941", "issn-l": "2041-1723"}, "abstract": "Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.", "doi": "10.1038/s41467-024-49131-1", "pmid": "38866781", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11169475"}, {"db": "pii", "key": "10.1038/s41467-024-49131-1"}], "notes": [], "created": "2026-09-23T09:04:19.641Z", "modified": "2026-09-23T09:04:21.364Z"}]}