{"entity": "researcher", "timestamp": "2026-08-20T21:22:41.670Z", "family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "affiliations": ["National Bioinformatics Infrastructure, SciLife Lab, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed"}}, "publications": [{"entity": "publication", "iuid": "84b68e9019d34d52aecd219380bdb7bb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/84b68e9019d34d52aecd219380bdb7bb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/84b68e9019d34d52aecd219380bdb7bb"}}, "title": "Single-Cell RNA Analysis Reveals Cell-Intrinsic Functions of CAR T Cells Correlating with Response in a Phase II Study of Lymphoma Patients.", "authors": [{"family": "Sar\u00e9n", "given": "Tina", "initials": "T", "orcid": "0000-0001-5227-6779", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed75a6dcbb9940e49481280af6183d69.json"}}, {"family": "Ramachandran", "given": "Mohanraj", "initials": "M", "orcid": "0000-0003-2685-0575", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dd947633edf949aba9e680e63a1e178b.json"}}, {"family": "Gammelg\u00e5rd", "given": "Gustav", "initials": "G", "orcid": "0009-0008-5522-0103", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/23c14e3d3bf445a7b3ff9d45eafbc5e1.json"}}, {"family": "L\u00f6vgren", "given": "Tanja", "initials": "T", "orcid": "0000-0003-2170-0682", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4a04a7c6e904ff0945c058fcaa3151d.json"}}, {"family": "Mirabello", "given": "Claudio", "initials": "C", "orcid": "0000-0001-7868-034X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4ca88bb3cf24e2591c447ec929502e6.json"}}, {"family": "Bj\u00f6rklund", "given": "\u00c5sa K", "initials": "\u00c5K", "orcid": "0000-0003-2224-7090", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cfd05eeb19640eea44f996699f998f0.json"}}, {"family": "Wikstr\u00f6m", "given": "Kristina", "initials": "K", "orcid": "0009-0000-1027-2534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1c1933e2ca1f494998c1681623af5ce9.json"}}, {"family": "Hashemi", "given": "Jamileh", "initials": "J", "orcid": "0000-0002-4997-3765", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2e89291626e4f3eb73835d352bf67f4.json"}}, {"family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed.json"}}, {"family": "Ahlstr\u00f6m", "given": "H\u00e5kan", "initials": "H", "orcid": "0000-0002-8701-969X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7e0504d2b7e14415b7c20229df25c0aa.json"}}, {"family": "Amini", "given": "Rose-Marie", "initials": "RM", "orcid": "0000-0003-0901-5252", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ae2f974dbafa4cdbbe268c635adcd278.json"}}, {"family": "Hagberg", "given": "Hans", "initials": "H", "orcid": "0000-0001-7855-2452", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f525112ab8e94ce790be483b56d7cbd6.json"}}, {"family": "Loskog", "given": "Angelica", "initials": "A", "orcid": "0000-0001-8583-6138", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/572e5b01b3bd48779252b9020732cb09.json"}}, {"family": "Enblad", "given": "Gunilla", "initials": "G", "orcid": "0000-0002-0594-724X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a285fb437854e2c998356b1a3124416.json"}}, {"family": "Essand", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9725-0422", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a2e56cdd77634c01ab6d98dea8c60430.json"}}], "type": "journal article", "published": "2023-10-13", "journal": {"title": "Clin. Cancer Res.", "issn": "1557-3265", "volume": "29", "issue": "20", "pages": "4139-4152", "issn-l": "1078-0432"}, "abstract": "Although CD19 chimeric antigen receptor T cells (CAR-T) therapy has shown remarkable success in B-cell malignancies, a substantial fraction of patients do not obtain a long-term clinical response. This could be influenced by the quality of the individual CAR-T infusion product. To shed some light on this, clinical outcome was correlated to characteristics of CAR-T infusion products.\n\nIn this phase II study, patients with B-cell lymphoma (n = 23) or leukemia (n = 1) received one or two infusions of third-generation CD19-directed CAR-Ts (2 \u00d7 108/m2). The clinical trial was registered at clinicaltrials.gov: NCT03068416. We investigated the transcriptional profile of individual CD19 CAR-T infusion products using targeted single-cell RNA sequencing and multicolor flow cytometry.\n\nTwo CAR-T infusions were not better than one in the settings used in this study. As for the CAR-T infusion products, we found that effector-like CD8+CAR-Ts with a high polyfunctionality, high cytotoxic and cytokine production profile, and low dysfunctional signature were associated with clinical response. An extended ex vivo expansion time during CAR-T manufacturing negatively influenced the proportion of effector CD8+CAR-Ts in the infusion product.\n\nWe identified cell-intrinsic characteristics of effector CD8+CAR-Ts correlating with response that could be used as an indicator for clinical outcome. The results in the study also serve as a guide to CAR-T manufacturing practices.", "doi": "10.1158/1078-0432.CCR-23-0178", "pmid": "37540566", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10570681"}, {"db": "pii", "key": "728314"}, {"db": "ClinicalTrials.gov", "key": "NCT03068416"}, {"db": "figshare", "key": "20208764"}], "notes": [], "created": "2026-08-20T12:12:20.782Z", "modified": "2026-08-20T12:12:21.097Z"}, {"entity": "publication", "iuid": "e4c03509339a4a7487c366bc3827bc47", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e4c03509339a4a7487c366bc3827bc47.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e4c03509339a4a7487c366bc3827bc47"}}, "title": "Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of Glioma.", "authors": [{"family": "Indira Chandran", "given": "Vineesh", "initials": "V"}, {"family": "Welinder", "given": "Charlotte", "initials": "C", "orcid": "0000-0001-9626-0576", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5715a70ce8df4a5d84ad88709791772a.json"}}, {"family": "M\u00e5nsson", "given": "Ann-Sofie", "initials": "AS"}, {"family": "Offer", "given": "Svenja", "initials": "S"}, {"family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed.json"}}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Lund", "given": "Sigrid M", "initials": "SM"}, {"family": "Pedersen", "given": "Shona", "initials": "S", "orcid": "0000-0001-6636-0293", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/daa11d544a2545b0aa2a7ec6083fc079.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Marko-Varga", "given": "Gyorgy", "initials": "G"}, {"family": "Johansson", "given": "Maria C", "initials": "MC"}, {"family": "Englund", "given": "Elisabet", "initials": "E", "orcid": "0000-0002-2708-2443", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8d415783747141269a6d9a843afc861d.json"}}, {"family": "Sundgren", "given": "Pia C", "initials": "PC", "orcid": "0000-0001-9237-1236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e580179d7d74958a5c1d509e581ddcc.json"}}, {"family": "Belting", "given": "Mattias", "initials": "M"}], "type": "journal article", "published": "2019-05-15", "journal": {"title": "Clin. Cancer Res.", "issn": "1557-3265", "volume": "25", "issue": "10", "pages": "3115-3127", "issn-l": "1078-0432"}, "abstract": "Liquid biopsy has great potential to improve the management of brain tumor patients at high risk of surgery-associated complications. Here, the aim was to explore plasma extracellular vesicle (plEV) immunoprofiling as a tool for noninvasive diagnosis of glioma.\n\nPlEV isolation and analysis were optimized using advanced mass spectrometry, nanoparticle tracking analysis, and electron microscopy. We then established a new procedure that combines size exclusion chromatography isolation and proximity extension assay-based ultrasensitive immunoprofiling of plEV proteins that was applied on a well-defined glioma study cohort (n = 82).\n\nAmong potential candidates, we for the first time identify syndecan-1 (SDC1) as a plEV constituent that can discriminate between high-grade glioblastoma multiforme (GBM, WHO grade IV) and low-grade glioma [LGG, WHO grade II; area under the ROC curve (AUC): 0.81; sensitivity: 71%; specificity: 91%]. These findings were independently validated by ELISA. Tumor SDC1 mRNA expression similarly discriminated between GBM and LGG in an independent glioma patient population from The Cancer Genome Atlas cohort (AUC: 0.91; sensitivity: 79%; specificity: 91%). In experimental studies with GBM cells, we show that SDC1 is efficiently sorted to secreted EVs. Importantly, we found strong support of plEVSDC1 originating from GBM tumors, as plEVSDC1 correlated with SDC1 protein expression in matched patient tumors, and plEVSDC1 was decreased postoperatively depending on the extent of surgery.\n\nOur studies support the concept of circulating plEVs as a tool for noninvasive diagnosis and monitoring of gliomas and should move this field closer to the goal of improving the management of cancer patients.", "doi": "10.1158/1078-0432.CCR-18-2946", "pmid": "30679164", "labels": [], "xrefs": [{"db": "pii", "key": "1078-0432.CCR-18-2946"}], "notes": [], "created": "2026-08-20T12:12:08.365Z", "modified": "2026-08-20T12:12:08.608Z"}]}