{"entity": "researcher", "timestamp": "2026-08-20T20:58:25.517Z", "family": "Chen", "given": "Yuanhuang", "initials": "Y", "orcid": "0000-0001-7404-5170", "affiliations": ["Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University; 1230 York Ave., New York, 10065, USA.", "Tri-Institutional PhD Program in Chemical Biology, New York, NY 10065, USA."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/127d5bd92dc249bca07717402dee4006.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/127d5bd92dc249bca07717402dee4006"}}, "publications": [{"entity": "publication", "iuid": "853228a414e14910b40095bc20ee75dd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/853228a414e14910b40095bc20ee75dd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/853228a414e14910b40095bc20ee75dd"}}, "title": "Multiplexed mapping of the interactome of GPCRs with receptor activity-modifying proteins.", "authors": [{"family": "Kotliar", "given": "Ilana B", "initials": "IB", "orcid": "0000-0001-9533-4828", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9066d33009cd4504a71287b07b54848e.json"}}, {"family": "Bendes", "given": "Annika", "initials": "A", "orcid": "0000-0001-9329-2353", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b84a8aa3e3b4439bb0b3a9fabdebcb70.json"}}, {"family": "Dahl", "given": "Leo", "initials": "L", "orcid": "0000-0003-1492-3052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f357e135f9e43999d78db11ae5f0f40.json"}}, {"family": "Chen", "given": "Yuanhuang", "initials": "Y", "orcid": "0000-0001-7404-5170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/127d5bd92dc249bca07717402dee4006.json"}}, {"family": "Saarinen", "given": "Marcus", "initials": "M"}, {"family": "Ceraudo", "given": "Emilie", "initials": "E", "orcid": "0000-0001-6563-9751", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1f8126992cd4ae9afc3852054d93270.json"}}, {"family": "Dodig-Crnkovi\u0107", "given": "Tea", "initials": "T", "orcid": "0000-0002-2875-896X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5365873ed6a7463485fb44550a4826ec.json"}}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ea446aa574042a295d5f69437402f76.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b01bbacfa24e4b9794734bf1121d1c38.json"}}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022e140799b6466ea123319c3d70c76e.json"}}, {"family": "Sakmar", "given": "Thomas P", "initials": "TP", "orcid": "0000-0002-2836-8953", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ff78d726542d46a4984572ea8fed0931.json"}}], "type": "journal article", "published": "2024-08-02", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "10", "issue": "31", "pages": "eado9959", "issn-l": "2375-2548"}, "abstract": "Receptor activity-modifying proteins (RAMPs) form complexes with G protein-coupled receptors (GPCRs) and may regulate their cellular trafficking and pharmacology. RAMP interactions have been identified for about 50 GPCRs, but only a few GPCR-RAMP complexes have been studied in detail. To elucidate a comprehensive GPCR-RAMP interactome, we created a library of 215 dual epitope-tagged (DuET) GPCRs representing all GPCR subfamilies and coexpressed each GPCR with each of the three RAMPs. Screening the GPCR-RAMP pairs with customized multiplexed suspension bead array (SBA) immunoassays, we identified 122 GPCRs that showed strong evidence for interaction with at least one RAMP. We screened for interactions in three cell lines and found 23 endogenously expressed GPCRs that formed complexes with RAMPs. Mapping the GPCR-RAMP interactome expands the current system-wide functional characterization of RAMP-interacting GPCRs to inform the design of selective therapeutics targeting GPCR-RAMP complexes.", "doi": "10.1126/sciadv.ado9959", "pmid": "39083597", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11290489"}], "notes": [], "created": "2026-08-20T11:58:48.742Z", "modified": "2026-08-20T11:58:48.924Z"}, {"entity": "publication", "iuid": "d258c9592d164cf2bda6526b2c87966c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d258c9592d164cf2bda6526b2c87966c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d258c9592d164cf2bda6526b2c87966c"}}, "title": "Expanding the GPCR-RAMP interactome.", "authors": [{"family": "Kotliar", "given": "Ilana B", "initials": "IB", "orcid": "0000-0001-9533-4828", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9066d33009cd4504a71287b07b54848e.json"}}, {"family": "Bendes", "given": "Annika", "initials": "A", "orcid": "0000-0001-9329-2353", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b84a8aa3e3b4439bb0b3a9fabdebcb70.json"}}, {"family": "Dahl", "given": "Leo", "initials": "L", "orcid": "0000-0003-1492-3052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f357e135f9e43999d78db11ae5f0f40.json"}}, {"family": "Chen", "given": "Yuanhuang", "initials": "Y", "orcid": "0000-0001-7404-5170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/127d5bd92dc249bca07717402dee4006.json"}}, {"family": "Saarinen", "given": "Marcus", "initials": "M", "orcid": "0000-0001-9019-8396", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e7fad8ab6acf406d940ae2af691a07a3.json"}}, {"family": "Ceraudo", "given": "Emilie", "initials": "E", "orcid": "0000-0001-6563-9751", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1f8126992cd4ae9afc3852054d93270.json"}}, {"family": "Dodig-Crnkovi\u0107", "given": "Tea", "initials": "T", "orcid": "0000-0002-2875-896X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5365873ed6a7463485fb44550a4826ec.json"}}, {"family": "Uhle'n", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ea446aa574042a295d5f69437402f76.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b01bbacfa24e4b9794734bf1121d1c38.json"}}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022e140799b6466ea123319c3d70c76e.json"}}, {"family": "Sakmar", "given": "Thomas P", "initials": "TP", "orcid": "0000-0002-2836-8953", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ff78d726542d46a4984572ea8fed0931.json"}}], "type": "preprint", "published": "2023-11-23", "journal": {"title": "bioRxiv", "issn": "2692-8205", "issn-l": null}, "abstract": "Receptor activity-modifying proteins (RAMPs) can form complexes with G protein-coupled receptors (GPCRs) and regulate their cellular trafficking and pharmacology. RAMP interactions have been identified for about 50 GPCRs, but only a few GPCR-RAMP complexes have been studied in detail. To elucidate a complete interactome between GPCRs and the three RAMPs, we developed a customized library of 215 Dual Epitope-Tagged (DuET) GPCRs representing all GPCR subfamilies. Using a multiplexed suspension bead array (SBA) assay, we identified 122 GPCRs that showed strong evidence for interaction with at least one RAMP. We screened for native interactions in three cell lines and found 23 GPCRs that formed complexes with RAMPs. Mapping the GPCR-RAMP interactome expands the current system-wide functional characterization of RAMP-interacting GPCRs to inform the design of selective GPCR-targeted therapeutics.", "doi": "10.1101/2023.11.22.568247", "pmid": "38045268", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10690247"}, {"db": "pii", "key": "2023.11.22.568247"}], "notes": [], "created": "2026-08-20T10:50:30.835Z", "modified": "2026-08-20T10:50:31.027Z"}]}