{"entity": "researcher", "timestamp": "2026-05-09T17:17:43.493Z", "family": "Javanmard", "given": "Mehdi", "initials": "M", "orcid": "0000-0002-6297-392X", "affiliations": ["Rutgers, The State University of New Jersey, New Brunswick, NJ, USA."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/1208a771a4fa451d92a74c76aa6032b2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/1208a771a4fa451d92a74c76aa6032b2"}}, "publications": [{"entity": "publication", "iuid": "76699bd557614e69bccb6e73592cd4a7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/76699bd557614e69bccb6e73592cd4a7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/76699bd557614e69bccb6e73592cd4a7"}}, "title": "Digital assay for rapid electronic quantification of clinical pathogens using DNA nanoballs.", "authors": [{"family": "Tayyab", "given": "Muhammad", "initials": "M", "orcid": "0000-0002-4372-504X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3fbced44ff6847fab3b50a830f44abc4.json"}}, {"family": "Barrett", "given": "Donal", "initials": "D", "orcid": "0000-0001-5381-5391", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7fb0f798fb9e4095af7146e8bc79feee.json"}}, {"family": "van Riel", "given": "Gijs", "initials": "G"}, {"family": "Liu", "given": "Shujing", "initials": "S", "orcid": "0000-0002-5783-4204", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/efd66c143bda4ad68ba21cd6e2319e15.json"}}, {"family": "Reinius", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0002-7021-5248", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a7c1abacc8d446bf8855254246b8d899.json"}}, {"family": "Scharfe", "given": "Curt", "initials": "C", "orcid": "0000-0001-6322-2039", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/151a81585ba4445793d52cf41934b10e.json"}}, {"family": "Griffin", "given": "Peter", "initials": "P"}, {"family": "Steinmetz", "given": "Lars M", "initials": "LM", "orcid": "0000-0002-3962-2865", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/19807fc4d94a44e088f35ef1e94e81ce.json"}}, {"family": "Javanmard", "given": "Mehdi", "initials": "M", "orcid": "0000-0002-6297-392X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1208a771a4fa451d92a74c76aa6032b2.json"}}, {"family": "Pelechano", "given": "Vicent", "initials": "V", "orcid": "0000-0002-9415-788X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1e893b93b7654d10b4072e1b5de0bd71.json"}}], "type": "journal article", "published": "2023-09-08", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "9", "issue": "36", "pages": "eadi4997", "issn-l": "2375-2548"}, "abstract": "Fast and accurate detection of nucleic acids is key for pathogen identification. Methods for DNA detection generally rely on fluorescent or colorimetric readout. The development of label-free assays decreases costs and test complexity. We present a novel method combining a one-pot isothermal generation of DNA nanoballs with their detection by electrical impedance. We modified loop-mediated isothermal amplification by using compaction oligonucleotides that self-assemble the amplified target into nanoballs. Next, we use capillary-driven flow to passively pass these nanoballs through a microfluidic impedance cytometer, thus enabling a fully compact system with no moving parts. The movement of individual nanoballs is detected by a change in impedance providing a quantized readout. This approach is flexible for the detection of DNA/RNA of numerous targets (severe acute respiratory syndrome coronavirus 2, HIV, \u03b2-lactamase gene, etc.), and we anticipate that its integration into a standalone device would provide an inexpensive (<$5), sensitive (10 target copies), and rapid test (<1 hour).", "doi": "10.1126/sciadv.adi4997", "pmid": "37672583", "labels": {"Vicent Pelechano": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC10482329"}], "notes": [], "created": "2023-11-23T09:13:47.169Z", "modified": "2023-11-23T09:13:47.417Z"}]}