{"entity": "researcher", "timestamp": "2026-08-25T09:31:30.101Z", "family": "Tybulewicz", "given": "Victor L J", "initials": "VLJ", "orcid": "0000-0003-2439-0798", "affiliations": ["The Francis Crick Institute, London, NW1 1AT, UK. Victor.T@crick.ac.uk."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/0d6ce13cb37e428291b4757806801cc4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/0d6ce13cb37e428291b4757806801cc4"}}, "publications": [{"entity": "publication", "iuid": "dde4ac25299344f9ae0cad9e456bc14f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/dde4ac25299344f9ae0cad9e456bc14f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/dde4ac25299344f9ae0cad9e456bc14f"}}, "title": "WNK1-dependent water influx is required for CD4+ T cell activation and T cell-dependent antibody responses.", "authors": [{"family": "Biggs O'May", "given": "Joshua", "initials": "J", "orcid": "0000-0002-3187-5550", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b0f69c6578fb40ee9a1bdf4489c39058.json"}}, {"family": "Vanes", "given": "Lesley", "initials": "L"}, {"family": "de Boer", "given": "Leonard L", "initials": "LL", "orcid": "0000-0002-9370-5290", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0eb6259b0f944ba3973a923dfb0f434b.json"}}, {"family": "Lewis", "given": "David A", "initials": "DA", "orcid": "0000-0001-7434-2837", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f2af89cc335432ca69bea70438ae012.json"}}, {"family": "Hartweger", "given": "Harald", "initials": "H", "orcid": "0000-0002-1118-1818", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2fdb7770dd8847739ad6ba60a948f666.json"}}, {"family": "Kunzelmann", "given": "Simone", "initials": "S", "orcid": "0000-0002-2678-0549", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea6f4647fab747bcb462288d7a2d2ff2.json"}}, {"family": "Hayward", "given": "Darryl", "initials": "D"}, {"family": "Llorian", "given": "Miriam", "initials": "M", "orcid": "0000-0003-2582-6602", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/352318e79d6b4e079e9521fac470e60e.json"}}, {"family": "K\u00f6chl", "given": "Robert", "initials": "R"}, {"family": "Tybulewicz", "given": "Victor L J", "initials": "VLJ", "orcid": "0000-0003-2439-0798", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0d6ce13cb37e428291b4757806801cc4.json"}}], "type": "journal article", "published": "2025-02-21", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "1857", "issn-l": "2041-1723"}, "abstract": "Signaling from the T cell antigen receptor (TCR) on CD4+ T cells plays a critical role in adaptive immune responses by inducing T cell activation, proliferation, and differentiation. Here we demonstrate that WNK1, a kinase implicated in osmoregulation in the kidney, is required in T cells to support T-dependent antibody responses. We show that the canonical WNK1-OXSR1-STK39 kinase signaling pathway is required for TCR signaling in CD4+ T cells, their subsequent entry into the cell cycle, and suppression of the ATR-mediated G2/M cell cycle checkpoint. We show that the WNK1 pathway regulates ion influx leading to water influx, potentially through AQP3, and that water influx is required for TCR-induced signaling and cell cycle entry. Thus, TCR signaling via WNK1, OXSR1, STK39 and AQP3 leads to water entry that is essential for CD4+ T cell proliferation and hence T cell-dependent antibody responses.", "doi": "10.1038/s41467-025-56778-x", "pmid": "39984435", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11845700"}, {"db": "pii", "key": "10.1038/s41467-025-56778-x"}], "notes": [], "created": "2026-08-21T11:50:03.139Z", "modified": "2026-08-21T11:50:03.438Z"}, {"entity": "publication", "iuid": "8a6382a368fe4519b837b43c9dd70b60", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8a6382a368fe4519b837b43c9dd70b60.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8a6382a368fe4519b837b43c9dd70b60"}}, "title": "T cell migration requires ion and water influx to regulate actin polymerization.", "authors": [{"family": "de Boer", "given": "Leonard L", "initials": "LL", "orcid": "0000-0002-9370-5290", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0eb6259b0f944ba3973a923dfb0f434b.json"}}, {"family": "Vanes", "given": "Lesley", "initials": "L"}, {"family": "Melgrati", "given": "Serena", "initials": "S"}, {"family": "Biggs O'May", "given": "Joshua", "initials": "J"}, {"family": "Hayward", "given": "Darryl", "initials": "D"}, {"family": "Driscoll", "given": "Paul C", "initials": "PC", "orcid": "0000-0002-4124-6950", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a9c2ace79e54606be1b45ff11f3803c.json"}}, {"family": "Day", "given": "Jason", "initials": "J", "orcid": "0000-0002-3078-0963", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8c24e7184b5240f6bdb9ba881040db7a.json"}}, {"family": "Griffiths", "given": "Alexander", "initials": "A"}, {"family": "Magueta", "given": "Renata", "initials": "R"}, {"family": "Morrell", "given": "Alexander", "initials": "A", "orcid": "0000-0003-3763-5846", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ba04683d2b94045ab16f7b70603a171.json"}}, {"family": "MacRae", "given": "James I", "initials": "JI", "orcid": "0000-0002-1464-8583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3c305abe065f415da2116d2c2ce06eeb.json"}}, {"family": "K\u00f6chl", "given": "Robert", "initials": "R"}, {"family": "Tybulewicz", "given": "Victor L J", "initials": "VLJ", "orcid": "0000-0003-2439-0798", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0d6ce13cb37e428291b4757806801cc4.json"}}], "type": "journal article", "published": "2023-12-06", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "7844", "issn-l": "2041-1723"}, "abstract": "Migration of T cells is essential for their ability to mount immune responses. Chemokine-induced T cell migration requires WNK1, a kinase that regulates ion influx into the cell. However, it is not known why ion entry is necessary for T cell movement. Here we show that signaling from the chemokine receptor CCR7 leads to activation of WNK1 and its downstream pathway at the leading edge of migrating CD4+ T cells, resulting in ion influx and water entry by osmosis. We propose that WNK1-induced water entry is required to swell the membrane at the leading edge, generating space into which actin filaments can polymerize, thereby facilitating forward movement of the cell. Given the broad expression of WNK1 pathway proteins, our study suggests that ion and water influx are likely to be essential for migration in many cell types, including leukocytes and metastatic tumor cells.", "doi": "10.1038/s41467-023-43423-8", "pmid": "38057317", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10700356"}, {"db": "pii", "key": "10.1038/s41467-023-43423-8"}], "notes": [], "created": "2026-08-21T11:49:40.167Z", "modified": "2026-08-21T11:49:40.466Z"}]}