{"entity": "researcher", "timestamp": "2026-08-20T20:47:11.485Z", "family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "affiliations": ["Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, 10691 Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f"}}, "publications": [{"entity": "publication", "iuid": "3d88996e69924c3e9569ca368c16309c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3d88996e69924c3e9569ca368c16309c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3d88996e69924c3e9569ca368c16309c"}}, "title": "Structure and dynamics of a multidomain ligand-gated ion channel revealed under acidic conditions", "authors": [{"family": "And\u00e9n", "given": "Olivia", "initials": "O", "orcid": "0009-0002-4816-5691", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6e5867d3d6bb48bb935cf2c28642c9a3.json"}}, {"family": "Rov\u0161nik", "given": "Ur\u0161ka", "initials": "U", "orcid": "0000-0001-5889-6899", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0ab87a8712f4ad886da61c1edcf679f.json"}}, {"family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}}, {"family": "Delarue", "given": "Marc", "initials": "M", "orcid": "0000-0003-0723-8102", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ee0e290455aa46c5a3a7d9f0ce226dcf.json"}}, {"family": "Howard", "given": "Rebecca J", "initials": "RJ", "orcid": "0000-0003-2049-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ce04593b5b24aaa8978fda1447dd0bf.json"}}, {"family": "Lindahl", "given": "Erik", "initials": "E", "orcid": "0000-0002-2734-2794", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9eb30fa60f9b4b95842ac9d9f3a0eaa9.json"}}], "type": "posted-content", "published": "2025-02-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.02.27.640520", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:01:20.993Z", "modified": "2026-08-20T11:01:21.080Z"}, {"entity": "publication", "iuid": "7ca5d3e48b684fdc984b3040f5d2f4fc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7ca5d3e48b684fdc984b3040f5d2f4fc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7ca5d3e48b684fdc984b3040f5d2f4fc"}}, "title": "Calcium stabilization of a flexible N-terminal domain in a pentameric ligand-gated ion channel", "authors": [{"family": "Fan", "given": "Chen", "initials": "C"}, {"family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}}, {"family": "Zhuang", "given": "Yuxuan", "initials": "Y", "orcid": "0000-0003-4390-8556", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61b8327a0f32416686748c52c38ecbf1.json"}}, {"family": "Howard", "given": "Rebecca J", "initials": "RJ", "orcid": "0000-0003-2049-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ce04593b5b24aaa8978fda1447dd0bf.json"}}, {"family": "Lindahl", "given": "Erik", "initials": "E", "orcid": "0000-0002-2734-2794", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9eb30fa60f9b4b95842ac9d9f3a0eaa9.json"}}], "type": "posted-content", "published": "2025-02-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.02.24.640012", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:01:10.325Z", "modified": "2026-08-20T11:01:10.354Z"}, {"entity": "publication", "iuid": "4ad049e949e64184b10da4d04e61db34", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4ad049e949e64184b10da4d04e61db34.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4ad049e949e64184b10da4d04e61db34"}}, "title": "Biophysical characterization of calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel.", "authors": [{"family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}}, {"family": "Rov\u0161nik", "given": "Ur\u0161ka", "initials": "U", "orcid": "0000-0001-5889-6899", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0ab87a8712f4ad886da61c1edcf679f.json"}}, {"family": "Hanke", "given": "Anton", "initials": "A", "orcid": "0000-0001-9552-1201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8fa04b7384b14cf1bfffe78493d643ce.json"}}, {"family": "Martel", "given": "Anne", "initials": "A"}, {"family": "Howard", "given": "Rebecca J", "initials": "RJ", "orcid": "0000-0003-2049-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ce04593b5b24aaa8978fda1447dd0bf.json"}}, {"family": "Lindahl", "given": "Erik", "initials": "E", "orcid": "0000-0002-2734-2794", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9eb30fa60f9b4b95842ac9d9f3a0eaa9.json"}}], "type": "journal article", "published": "2022-12-13", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "119", "issue": "50", "pages": "e2210669119", "issn-l": "0027-8424"}, "abstract": "Pentameric ligand-gated ion channels (pLGICs) perform electrochemical signal transduction in organisms ranging from bacteria to humans. Among the prokaryotic pLGICs, there is architectural diversity involving N-terminal domains (NTDs) not found in eukaryotic relatives, exemplified by the calcium-sensitive channel (DeCLIC) from a Desulfofustis deltaproteobacterium, which has an NTD in addition to the canonical pLGIC structure. Here, we have characterized the structure and dynamics of DeCLIC through cryoelectron microscopy (cryo-EM), small-angle neutron scattering (SANS), and molecular dynamics (MD) simulations. In the presence and absence of calcium, cryo-EM yielded structures with alternative conformations of the calcium-binding site. SANS profiles further revealed conformational diversity at room temperature beyond that observed in static structures, shown through MD to be largely attributable to rigid-body motions of the NTD relative to the protein core, with expanded and asymmetric conformations improving the fit of the SANS data. This work reveals the range of motion available to the DeCLIC NTD and calcium-binding site, expanding the conformational landscape of the pLGIC family. Further, these findings demonstrate the power of combining low-resolution scattering, high-resolution structural, and MD simulation data to elucidate interfacial interactions that are highly conserved in the pLGIC family.", "doi": "10.1073/pnas.2210669119", "pmid": "36480474", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9897478"}], "notes": [], "created": "2026-08-20T09:31:21.845Z", "modified": "2026-08-20T09:31:21.946Z"}, {"entity": "publication", "iuid": "20371f6d8c644414a6ddc4efd677a764", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/20371f6d8c644414a6ddc4efd677a764.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/20371f6d8c644414a6ddc4efd677a764"}}, "title": "Automated simulation-based membrane-protein refinement into cryo-EM data", "authors": [{"family": "Yvonnesdotter", "given": "Linnea", "initials": "L", "orcid": "0000-0001-7163-7488", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/546941b1ef034cbeb4a16fa9cd9948aa.json"}}, {"family": "Rov\u0161nik", "given": "Ur\u0161ka", "initials": "U", "orcid": "0000-0001-5889-6899", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0ab87a8712f4ad886da61c1edcf679f.json"}}, {"family": "Blau", "given": "Christian", "initials": "C", "orcid": "0000-0003-2558-0497", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/75324408cf1c44538571789269dff9cb.json"}}, {"family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}}, {"family": "Howard", "given": "Rebecca J", "initials": "RJ", "orcid": "0000-0003-2049-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ce04593b5b24aaa8978fda1447dd0bf.json"}}, {"family": "Lindahl", "given": "Erik", "initials": "E", "orcid": "0000-0002-2734-2794", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9eb30fa60f9b4b95842ac9d9f3a0eaa9.json"}}], "type": "posted-content", "published": "2022-10-31", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.10.28.514175", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:42:00.493Z", "modified": "2026-08-20T10:42:00.591Z"}, {"entity": "publication", "iuid": "86a390ff753f425fa460e1089cb900a2", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/86a390ff753f425fa460e1089cb900a2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/86a390ff753f425fa460e1089cb900a2"}}, "title": "Biophysical characterization of calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel", "authors": [{"family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}}, {"family": "Rov\u0161nik", "given": "Ur\u0161ka", "initials": "U", "orcid": "0000-0001-5889-6899", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0ab87a8712f4ad886da61c1edcf679f.json"}}, {"family": "Hanke", "given": "Anton", "initials": "A", "orcid": "0000-0001-9552-1201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8fa04b7384b14cf1bfffe78493d643ce.json"}}, {"family": "Martel", "given": "Anne", "initials": "A"}, {"family": "Howard", "given": "Rebecca J", "initials": "RJ", "orcid": "0000-0003-2049-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ce04593b5b24aaa8978fda1447dd0bf.json"}}, {"family": "Lindahl", "given": "Erik", "initials": "E", "orcid": "0000-0002-2734-2794", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9eb30fa60f9b4b95842ac9d9f3a0eaa9.json"}}], "type": "posted-content", "published": "2022-05-06", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.05.06.490775", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:11:04.419Z", "modified": "2026-08-20T10:11:04.497Z"}, {"entity": "publication", "iuid": "5c28dc8f28bb4a51bd6d7c86acf4bbda", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5c28dc8f28bb4a51bd6d7c86acf4bbda.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5c28dc8f28bb4a51bd6d7c86acf4bbda"}}, "title": "Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering.", "authors": [{"family": "Lycksell", "given": "Marie", "initials": "M", "orcid": "0000-0003-3271-7973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0833a696c9ec497bae99bbe631af624f.json"}}, {"family": "Rov\u0161nik", "given": "Ur\u0161ka", "initials": "U", "orcid": "0000-0001-5889-6899", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0ab87a8712f4ad886da61c1edcf679f.json"}}, {"family": "Bergh", "given": "Cathrine", "initials": "C", "orcid": "0000-0001-7540-5887", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c8a3efa976b41edb6ace4efae952125.json"}}, {"family": "Johansen", "given": "Nicolai T", "initials": "NT"}, {"family": "Martel", "given": "Anne", "initials": "A"}, {"family": "Porcar", "given": "Lionel", "initials": "L"}, {"family": "Arleth", "given": "Lise", "initials": "L", "orcid": "0000-0002-4694-4299", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6c2d657efee34051a6d80a6ca776b076.json"}}, {"family": "Howard", "given": "Rebecca J", "initials": "RJ", "orcid": "0000-0003-2049-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ce04593b5b24aaa8978fda1447dd0bf.json"}}, {"family": "Lindahl", "given": "Erik", "initials": "E"}], "type": "journal article", "published": "2021-09-14", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "118", "issue": "37", "issn-l": "0027-8424"}, "abstract": "Pentameric ligand-gated ion channels undergo subtle conformational cycling to control electrochemical signal transduction in many kingdoms of life. Several crystal structures have now been reported in this family, but the functional relevance of such models remains unclear. Here, we used small-angle neutron scattering (SANS) to probe ambient solution-phase properties of the pH-gated bacterial ion channel GLIC under resting and activating conditions. Data collection was optimized by inline paused-flow size-exclusion chromatography, and exchanging into deuterated detergent to hide the micelle contribution. Resting-state GLIC was the best-fit crystal structure to SANS curves, with no evidence for divergent mechanisms. Moreover, enhanced-sampling molecular-dynamics simulations enabled differential modeling in resting versus activating conditions, with the latter corresponding to an intermediate ensemble of both the extracellular and transmembrane domains. This work demonstrates state-dependent changes in a pentameric ion channel by SANS, an increasingly accessible method for macromolecular characterization with the coming generation of neutron sources.", "doi": "10.1073/pnas.2108006118", "pmid": "34504004", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8449418"}, {"db": "pii", "key": "2108006118"}], "notes": [], "created": "2026-08-20T09:30:59.463Z", "modified": "2026-08-20T09:30:59.654Z"}]}