{"entity": "researcher", "timestamp": "2026-08-23T09:27:05.355Z", "family": "Shibuya", "given": "Hiroki", "initials": "H", "orcid": "0000-0002-3400-0741", "affiliations": ["Department of Chemistry and Molecular Biology, University of Gothenburg, 41390, Gothenburg, Sweden. hiroki.shibuya@riken.jp.", "Laboratory for Gametogenesis, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe, Japan. hiroki.shibuya@riken.jp."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/06ba5c9430a8424bbd027c165e2c11f1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/06ba5c9430a8424bbd027c165e2c11f1"}}, "publications": [{"entity": "publication", "iuid": "fb57b46c2808462d9b5c3359386a01d1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fb57b46c2808462d9b5c3359386a01d1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fb57b46c2808462d9b5c3359386a01d1"}}, "title": "CCDC28A deficiency causes head-tail coupling defects and immotility in murine spermatozoa.", "authors": [{"family": "Stojanovic", "given": "Nena", "initials": "N"}, {"family": "Hern\u00e1ndez", "given": "Rosario Ortiz", "initials": "RO"}, {"family": "Ram\u00edrez", "given": "Nayeli Torres", "initials": "NT"}, {"family": "Mart\u00ednez", "given": "Olga Margarita Echeverr\u00eda", "initials": "OME"}, {"family": "Hern\u00e1ndez", "given": "Abrahan Hern\u00e1ndez", "initials": "AH"}, {"family": "Shibuya", "given": "Hiroki", "initials": "H", "orcid": "0000-0002-3400-0741", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06ba5c9430a8424bbd027c165e2c11f1.json"}}], "type": "journal article", "published": "2024-11-05", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "14", "issue": "1", "pages": "26808", "issn-l": "2045-2322"}, "abstract": "Male infertility presents a substantial challenge in reproductive medicine, often attributed to impaired sperm motility. The present study investigates the role of CCDC28A, a protein expressed specifically in male germ cells, whose paralog CCDC28B has been implicated in ciliogenesis. We identify unique expression patterns for CCDC28A and CCDC28B within the mouse testes, where CCDC28A is expressed in germ cells, whereas CCDC28B is expressed in supporting somatic cells. Through knockout mouse models and histological analyses, we reveal that CCDC28A deficiency results in diminished sperm motility and structural aberrations in sperm tails, notably affecting the head-tail coupling apparatus (HTCA), thereby causing male infertility. Fine structural analyses by transmission electron microscopy reveal disruptions at the capitulum-basal plate junction of the HTCA in the CCDC28A mutants. This results in the bending of the head within the neck region, often accompanied by thickening of the tail midpiece. Our discovery demonstrates that CCDC28A plays an essential role in male fertility and sperm tail morphogenesis through the formation of HTCA.", "doi": "10.1038/s41598-024-78453-9", "pmid": "39500989", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11538371"}, {"db": "pii", "key": "10.1038/s41598-024-78453-9"}], "notes": [], "created": "2026-08-21T11:54:10.057Z", "modified": "2026-08-21T11:54:10.104Z"}, {"entity": "publication", "iuid": "bd3ebf44bf0a43b192d97874a53728eb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bd3ebf44bf0a43b192d97874a53728eb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bd3ebf44bf0a43b192d97874a53728eb"}}, "title": "Regulation of meiotic telomere dynamics through membrane fluidity promoted by AdipoR2-ELOVL2.", "authors": [{"family": "Zhang", "given": "Jingjing", "initials": "J"}, {"family": "Ruiz", "given": "Mario", "initials": "M"}, {"family": "Bergh", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0001-9993-6965", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0340d8f630034124a106f49ebd3101f3.json"}}, {"family": "Henricsson", "given": "Marcus", "initials": "M", "orcid": "0000-0002-4202-0339", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e0fbd663456e4767acbfbe87f79a930b.json"}}, {"family": "Stojanovi\u0107", "given": "Nena", "initials": "N"}, {"family": "Devkota", "given": "Ranjan", "initials": "R"}, {"family": "Henn", "given": "Marius", "initials": "M"}, {"family": "Bohlooly-Y", "given": "Mohammad", "initials": "M"}, {"family": "Hern\u00e1ndez-Hern\u00e1ndez", "given": "Abrahan", "initials": "A"}, {"family": "Alsheimer", "given": "Manfred", "initials": "M", "orcid": "0000-0002-6646-556X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7a2c8d4c716b4c3ca8be4dc3e2e7da6a.json"}}, {"family": "Bor\u00e9n", "given": "Jan", "initials": "J", "orcid": "0000-0003-0786-8091", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/73a770eb9e614448a046b8e9199052e9.json"}}, {"family": "Pilon", "given": "Marc", "initials": "M", "orcid": "0000-0003-3919-2882", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c5fc819e1734531b662ae2fb96fc17e.json"}}, {"family": "Shibuya", "given": "Hiroki", "initials": "H", "orcid": "0000-0002-3400-0741", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06ba5c9430a8424bbd027c165e2c11f1.json"}}], "type": "journal article", "published": "2024-03-14", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "2315", "issn-l": "2041-1723"}, "abstract": "The cellular membrane in male meiotic germ cells contains a unique class of phospholipids and sphingolipids that is required for male reproduction. Here, we show that a conserved membrane fluidity sensor, AdipoR2, regulates the meiosis-specific lipidome in mouse testes by promoting the synthesis of sphingolipids containing very-long-chain polyunsaturated fatty acids (VLC-PUFAs). AdipoR2 upregulates the expression of a fatty acid elongase, ELOVL2, both transcriptionally and post-transcriptionally, to synthesize VLC-PUFA. The depletion of VLC-PUFAs and subsequent accumulation of palmitic acid in AdipoR2 knockout testes stiffens the cellular membrane and causes the invagination of the nuclear envelope. This condition impairs the nuclear peripheral distribution of meiotic telomeres, leading to errors in homologous synapsis and recombination. Further, the stiffened membrane impairs the formation of intercellular bridges and the germ cell syncytium, which disrupts the orderly arrangement of cell types within the seminiferous tubules. According to our findings we propose a framework in which the highly-fluid membrane microenvironment shaped by AdipoR2-ELOVL2 underpins meiosis-specific chromosome dynamics in testes.", "doi": "10.1038/s41467-024-46718-6", "pmid": "38485951", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10940294"}, {"db": "pii", "key": "10.1038/s41467-024-46718-6"}], "notes": [], "created": "2026-08-21T11:49:42.643Z", "modified": "2026-08-21T11:49:42.835Z"}]}