{"entity": "researcher", "timestamp": "2026-08-20T20:47:32.506Z", "family": "Bonneuil", "given": "Willy V", "initials": "WV", "orcid": "0000-0002-6147-5937", "affiliations": ["Department of Engineering Mechanics, KTH Royal Institute of Technology, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/05a9242ab69141fa88d36fa21e4d0cc9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/05a9242ab69141fa88d36fa21e4d0cc9"}}, "publications": [{"entity": "publication", "iuid": "dbe6306927ed4058b9f435c997d2bf5f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/dbe6306927ed4058b9f435c997d2bf5f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/dbe6306927ed4058b9f435c997d2bf5f"}}, "title": "Capacity and limitations of microfluidic flow to increase solute transport in three-dimensional cell cultures.", "authors": [{"family": "Bonneuil", "given": "Willy V", "initials": "WV", "orcid": "0000-0002-6147-5937", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/05a9242ab69141fa88d36fa21e4d0cc9.json"}}, {"family": "Katiyar", "given": "Neeraj", "initials": "N"}, {"family": "Tenje", "given": "Maria", "initials": "M"}, {"family": "Bagheri", "given": "Shervin", "initials": "S", "orcid": "0000-0002-8209-1449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3a536948725048f6ad36af1bbd70afa1.json"}}], "type": "journal article", "published": "2025-01-00", "journal": {"title": "J R Soc Interface", "issn": "1742-5662", "volume": "22", "issue": "222", "pages": "20240463", "issn-l": null}, "abstract": "Culturing living cells in three-dimensional environments increases the biological relevance of laboratory experiments, but requires solutes to overcome a diffusion barrier to reach the centre of cellular constructs. We present a theoretical and numerical investigation that brings a mechanistic understanding of how microfluidic culture conditions, including chamber size, inlet fluid velocity and spatial confinement, affect solute distribution within three-dimensional cellular constructs. Contact with the chamber substrate reduces the maximally achievable construct radius by 15%. In practice, finite diffusion and convection kinetics in the microfluidic chamber further lower that limit. The benefits of external convection are greater if transport rates across diffusion-dominated areas are high. Those are omnipresent and include the diffusive boundary layer growing from the fluid-construct interface and regions near corners where fluid is recirculating. Such regions multiply the required convection to achieve a given solute penetration by up to 100, so chip designs ought to minimize them. Our results define conditions where complete solute transport into an avascular three-dimensional cell construct is achievable and applies to real chambers without needing to simulate their exact geometries.", "doi": "10.1098/rsif.2024.0463", "pmid": "39875093", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11774591"}], "notes": [], "created": "2026-08-20T09:52:54.598Z", "modified": "2026-08-20T09:52:54.691Z"}]}