{"entity": "researcher", "timestamp": "2026-09-12T08:29:46.758Z", "family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "affiliations": ["Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6"}}, "publications": [{"entity": "publication", "iuid": "2d490447288c4a31949511648905f5c8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2d490447288c4a31949511648905f5c8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2d490447288c4a31949511648905f5c8"}}, "title": "The pcnB gene sustains Shigella flexneri virulence", "authors": [{"family": "Frisch", "given": "Thibault", "initials": "T", "orcid": "0009-0005-4919-3557", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a098a65e67a649539154c1ae75296184.json"}}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Komi", "given": "Margarita", "initials": "M"}, {"family": "Karlsson", "given": "Philip A", "initials": "PA", "orcid": "0000-0003-0060-005X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c7cd878b190e4a50adb44c66db5d02e4.json"}}, {"family": "Bhetwal", "given": "Anjeela", "initials": "A", "orcid": "0000-0001-7739-8908", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51d09ce468a84276a1c7742d3e561844.json"}}, {"family": "Jenniches", "given": "Laura", "initials": "L", "orcid": "0000-0001-8455-3195", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f76df467a3364875b6c610ec885f5a76.json"}}, {"family": "Barquist", "given": "Lars", "initials": "L", "orcid": "0000-0003-4732-2667", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a825c68114764722aed640d984680457.json"}}, {"family": "Holmqvist", "given": "Erik", "initials": "E", "orcid": "0000-0001-7834-1487", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/09deb8319a1245c7aa22664aa66ffd51.json"}}, {"family": "Mateus", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-6870-0677", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebf0d242aa894a66b60828456bbc22aa.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}], "type": "posted-content", "published": "2025-06-16", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.06.16.659909", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:06:10.369Z", "modified": "2026-08-20T11:06:10.523Z"}, {"entity": "publication", "iuid": "7f26c631843d48329e7f00e76902bfe6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7f26c631843d48329e7f00e76902bfe6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7f26c631843d48329e7f00e76902bfe6"}}, "title": "Determinants of the Divergent Salmonella and Shigella Epithelial Colonization Strategies Resolved in Human Enteroids and Colonoids", "authors": [{"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Martino", "given": "Maria Letizia Di", "initials": "MLD", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Lopes", "given": "Ana C C", "initials": "ACC", "orcid": "0000-0002-9135-7974", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe2f84d7de874a92aa3992cd8703f227.json"}}, {"family": "Bergholtz", "given": "Alexandra", "initials": "A", "orcid": "0000-0002-1630-4442", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21b09621be1d4fe5a3c956360234b99d.json"}}, {"family": "Sundbom", "given": "Magnus", "initials": "M", "orcid": "0000-0002-6243-2859", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dec44b4c4ce84ce6b04bf9506874246b.json"}}, {"family": "Skogar", "given": "Martin", "initials": "M", "orcid": "0000-0002-6840-2749", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2231651ebc9c4e64bad27ece4086d05f.json"}}, {"family": "Graf", "given": "Wilhelm", "initials": "W", "orcid": "0000-0001-7042-849X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d657a0633d547ab994bfaafd5b63620.json"}}, {"family": "Webb", "given": "Dominic Luc", "initials": "DL", "orcid": "0000-0002-6979-9194", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f8caab45e535418aa431bc55eca471e8.json"}}, {"family": "Hellstr\u00f6m", "given": "Per M", "initials": "PM", "orcid": "0000-0001-8428-0772", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9627ff4daff4db2802135e0d06282e0.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "posted-content", "published": "2024-05-03", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.05.03.592388", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:54:48.002Z", "modified": "2026-08-20T10:54:48.227Z"}, {"entity": "publication", "iuid": "2d6d03aaf58242b6b95f18bddc3092a8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2d6d03aaf58242b6b95f18bddc3092a8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2d6d03aaf58242b6b95f18bddc3092a8"}}, "title": "A two-step activation mechanism enables mast cells to differentiate their response between extracellular and invasive enterobacterial infection.", "authors": [{"family": "von Beek", "given": "Christopher", "initials": "C", "orcid": "0000-0001-6310-7583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fba39ea4c0f74e65a1a61e1ad1fb7b73.json"}}, {"family": "Fahlgren", "given": "Anna", "initials": "A"}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Lindahl", "given": "Otto", "initials": "O", "orcid": "0000-0001-7518-9483", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9438c537a23642f5b5269f3428b2b9f8.json"}}, {"family": "Prensa", "given": "Grisna I", "initials": "GI", "orcid": "0009-0002-7101-7224", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e90e9ea8e5924e219ceb2fdfd9c6c113.json"}}, {"family": "Mendez-Enriquez", "given": "Erika", "initials": "E", "orcid": "0000-0002-2114-2812", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ab7409b0b124890a689f4b7b1cc06db.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Hallgren", "given": "Jenny", "initials": "J", "orcid": "0000-0002-3685-5364", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6c9f67368cbc4ebd929f38f4bca9da03.json"}}, {"family": "F\u00e4llman", "given": "Maria", "initials": "M", "orcid": "0000-0001-6874-6384", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e36dc965299248a5a309013213e2fb44.json"}}, {"family": "Pejler", "given": "Gunnar", "initials": "G"}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "journal article", "published": "2024-01-30", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "904", "issn-l": "2041-1723"}, "abstract": "Mast cells localize to mucosal tissues and contribute to innate immune defense against infection. How mast cells sense, differentiate between, and respond to bacterial pathogens remains a topic of ongoing debate. Using the prototype enteropathogen Salmonella Typhimurium (S.Tm) and other related enterobacteria, here we show that mast cells can regulate their cytokine secretion response to distinguish between extracellular and invasive bacterial infection. Tissue-invasive S.Tm and mast cells colocalize in the mouse gut during acute Salmonella infection. Toll-like Receptor 4 (TLR4) sensing of extracellular S.Tm, or pure lipopolysaccharide, causes a modest induction of cytokine transcripts and proteins, including IL-6, IL-13, and TNF. By contrast, type-III-secretion-system-1 (TTSS-1)-dependent S.Tm invasion of both mouse and human mast cells triggers rapid and potent inflammatory gene expression and >100-fold elevated cytokine secretion. The S.Tm TTSS-1 effectors SopB, SopE, and SopE2 here elicit a second activation signal, including Akt phosphorylation downstream of effector translocation, which combines with TLR activation to drive the full-blown mast cell response. Supernatants from S.Tm-infected mast cells boost macrophage survival and maturation from bone-marrow progenitors. Taken together, this study shows that mast cells can differentiate between extracellular and host-cell invasive enterobacteria via a two-step activation mechanism and tune their inflammatory output accordingly.", "doi": "10.1038/s41467-024-45057-w", "pmid": "38291037", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC10828507"}, {"db": "pii", "key": "10.1038/s41467-024-45057-w"}], "notes": [], "created": "2024-11-25T16:25:09.599Z", "modified": "2024-11-25T16:25:09.822Z"}, {"entity": "publication", "iuid": "49b8df584df34de7886ba6899e08f188", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/49b8df584df34de7886ba6899e08f188.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/49b8df584df34de7886ba6899e08f188"}}, "title": "Gasdermin D is the only Gasdermin that provides protection against acute Salmonella gut infection in mice.", "authors": [{"family": "Fattinger", "given": "Stefan A", "initials": "SA", "orcid": "0000-0003-4899-4414", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a3891756a0e46b7be8494556da9254f.json"}}, {"family": "Maurer", "given": "Luca", "initials": "L", "orcid": "0000-0002-9498-2775", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b81ff934767f4795b2244d13f9fb7ad5.json"}}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Bernard", "given": "Elliott M", "initials": "EM", "orcid": "0000-0003-2761-3790", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c733edbded147bd80973f9027e27208.json"}}, {"family": "Enz", "given": "Ursina", "initials": "U", "orcid": "0000-0002-4220-2650", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5242cda93ba54b34a969903d49cdda25.json"}}, {"family": "Ganguillet", "given": "Suwannee", "initials": "S"}, {"family": "G\u00fcl", "given": "Ersin", "initials": "E", "orcid": "0000-0003-2873-8034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc90347a80ca48e28d2ab8293ce293e9.json"}}, {"family": "Kroon", "given": "Sanne", "initials": "S", "orcid": "0000-0002-5722-0763", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ee4762c9bf94dd0a583281a4963cc35.json"}}, {"family": "Demarco", "given": "Benjamin", "initials": "B"}, {"family": "Mack", "given": "Vanessa", "initials": "V"}, {"family": "Furter", "given": "Markus", "initials": "M", "orcid": "0000-0003-4073-2268", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51f09daa062347adb84d145a0128c608.json"}}, {"family": "Barthel", "given": "Manja", "initials": "M"}, {"family": "Pelczar", "given": "Pawel", "initials": "P"}, {"family": "Shao", "given": "Feng", "initials": "F", "orcid": "0000-0002-9562-7791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e64e1601b52746f5b81e2331a9bc58ab.json"}}, {"family": "Broz", "given": "Petr", "initials": "P", "orcid": "0000-0002-2334-7790", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/63cd5a8b81864b83bf81d9a0ed7a8041.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Hardt", "given": "Wolf-Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}], "type": "journal article", "published": "2023-11-28", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "120", "issue": "48", "pages": "e2315503120", "issn-l": "0027-8424"}, "abstract": "Gasdermins (GSDMs) share a common functional domain structure and are best known for their capacity to form membrane pores. These pores are hallmarks of a specific form of cell death called pyroptosis and mediate the secretion of pro-inflammatory cytokines such as interleukin 1\u03b2 (IL1\u03b2) and interleukin 18 (IL18). Thereby, Gasdermins have been implicated in various immune responses against cancer and infectious diseases such as acute Salmonella Typhimurium (S.Tm) gut infection. However, to date, we lack a comprehensive functional assessment of the different Gasdermins (GSDMA-E) during S.Tm infection in vivo. Here, we used epithelium-specific ablation, bone marrow chimeras, and mouse lines lacking individual Gasdermins, combinations of Gasdermins or even all Gasdermins (GSDMA1-3C1-4DE) at once and performed littermate-controlled oral S.Tm infections in streptomycin-pretreated mice to investigate the impact of all murine Gasdermins. While GSDMA, C, and E appear dispensable, we show that GSDMD i) restricts S.Tm loads in the gut tissue and systemic organs, ii) controls gut inflammation kinetics, and iii) prevents epithelium disruption by 72 h of the infection. Full protection requires GSDMD expression by both bone-marrow-derived lamina propria cells and intestinal epithelial cells (IECs). In vivo experiments as well as 3D-, 2D-, and chimeric enteroid infections further show that infected IEC extrusion proceeds also without GSDMD, but that GSDMD controls the permeabilization and morphology of the extruding IECs, affects extrusion kinetics, and promotes overall mucosal barrier capacity. As such, this work identifies a unique multipronged role of GSDMD among the Gasdermins for mucosal tissue defense against a common enteric pathogen.", "doi": "10.1073/pnas.2315503120", "pmid": "37988464", "labels": {"SciLifeLab Fellow": null, "Mikael Sellin": null}, "xrefs": [], "notes": [], "created": "2023-12-01T13:09:50.356Z", "modified": "2023-12-01T13:09:50.555Z"}, {"entity": "publication", "iuid": "c2edd04be55b41629836b51cdcbb1849", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c2edd04be55b41629836b51cdcbb1849.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c2edd04be55b41629836b51cdcbb1849"}}, "title": "Trophozoite fitness dictates the intestinal epithelial cell response to Giardia intestinalis infection.", "authors": [{"family": "Gr\u00fcttner", "given": "Jana", "initials": "J", "orcid": "0000-0002-2507-3375", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8bec9e9a2f4b5a92ae3a2aa6f57e7c.json"}}, {"family": "van Rijn", "given": "Jorik M", "initials": "JM", "orcid": "0000-0003-2865-4455", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/198177b1db8d4e8d867e86e3b3770aea.json"}}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Florbrant", "given": "Alexandra", "initials": "A", "orcid": "0000-0002-1630-4442", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21b09621be1d4fe5a3c956360234b99d.json"}}, {"family": "Webb", "given": "Dominic-Luc", "initials": "DL", "orcid": "0000-0002-6979-9194", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f8caab45e535418aa431bc55eca471e8.json"}}, {"family": "Hellstr\u00f6m", "given": "Per M", "initials": "PM", "orcid": "0000-0001-8428-0772", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9627ff4daff4db2802135e0d06282e0.json"}}, {"family": "Sundbom", "given": "Magnus", "initials": "M", "orcid": "0000-0002-6243-2859", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dec44b4c4ce84ce6b04bf9506874246b.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Sv\u00e4rd", "given": "Staffan G", "initials": "SG", "orcid": "0000-0002-7392-1746", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d3542be86984e928373e248f1c88b47.json"}}], "type": "journal article", "published": "2023-05-00", "journal": {"title": "PLoS Pathog", "issn": "1553-7374", "volume": "19", "issue": "5", "pages": "e1011372", "issn-l": "1553-7366"}, "abstract": "Giardia intestinalis is a non-invasive, protozoan parasite infecting the upper small intestine of most mammals. Symptomatic infections cause the diarrhoeal disease giardiasis in humans and animals, but at least half of the infections are asymptomatic. However, the molecular underpinnings of these different outcomes of the infection are still poorly defined. Here, we studied the early transcriptional response to G. intestinalis trophozoites, the disease-causing life-cycle stage, in human enteroid-derived, 2-dimensional intestinal epithelial cell (IEC) monolayers. Trophozoites preconditioned in media that maximise parasite fitness triggered only neglectable inflammatory transcription in the IECs during the first hours of co-incubation. By sharp contrast, \"non-fit\" or lysed trophozoites induced a vigorous IEC transcriptional response, including high up-regulation of many inflammatory cytokines and chemokines. Furthermore, \"fit\" trophozoites could even suppress the stimulatory effect of lysed trophozoites in mixed infections, suggesting active G. intestinalis suppression of the IEC response. By dual-species RNA-sequencing, we defined the IEC and G. intestinalis gene expression programs associated with these differential outcomes of the infection. Taken together, our results inform on how G. intestinalis infection can lead to such highly variable effects on the host, and pinpoints trophozoite fitness as a key determinant of the IEC response to this common parasite.", "doi": "10.1371/journal.ppat.1011372", "pmid": "37141303", "labels": {"SciLifeLab Fellow": null, "Mikael Sellin": null}, "xrefs": [{"db": "pmc", "key": "PMC10187934"}, {"db": "pii", "key": "PPATHOGENS-D-22-02213"}], "notes": [], "created": "2023-12-01T13:09:43.844Z", "modified": "2023-12-01T13:09:43.926Z"}, {"entity": "publication", "iuid": "b1c8b2d1359f4b6e8bad357716c68757", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b1c8b2d1359f4b6e8bad357716c68757.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b1c8b2d1359f4b6e8bad357716c68757"}}, "title": "Gasdermin D is the only Gasdermin that provides non-redundant protection against acute Salmonella gut infection", "authors": [{"family": "Fattinger", "given": "Stefan A", "initials": "SA", "orcid": "0000-0003-4899-4414", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a3891756a0e46b7be8494556da9254f.json"}}, {"family": "Maurer", "given": "Luca", "initials": "L"}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Enz", "given": "Ursina", "initials": "U"}, {"family": "Ganguillet", "given": "Suwannee", "initials": "S"}, {"family": "G\u00fcl", "given": "Ersin", "initials": "E", "orcid": "0000-0003-2873-8034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc90347a80ca48e28d2ab8293ce293e9.json"}}, {"family": "Kroon", "given": "Sanne", "initials": "S", "orcid": "0000-0002-5722-0763", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ee4762c9bf94dd0a583281a4963cc35.json"}}, {"family": "Demarco", "given": "Benjamin", "initials": "B", "orcid": "0000-0002-5537-1381", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7302de742b394a16be37cd56e010f22a.json"}}, {"family": "Furter", "given": "Markus", "initials": "M", "orcid": "0000-0003-4073-2268", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51f09daa062347adb84d145a0128c608.json"}}, {"family": "Barthel", "given": "Manja", "initials": "M", "orcid": "0000-0001-6771-7661", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/adc1778ad09f4333abd2f45a29c113c1.json"}}, {"family": "Pelczar", "given": "Pawel", "initials": "P"}, {"family": "Shao", "given": "Feng", "initials": "F", "orcid": "0000-0002-9562-7791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e64e1601b52746f5b81e2331a9bc58ab.json"}}, {"family": "Broz", "given": "Petr", "initials": "P", "orcid": "0000-0002-2334-7790", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/63cd5a8b81864b83bf81d9a0ed7a8041.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Hardt", "given": "Wolf Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}], "type": "posted-content", "published": "2022-11-25", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.11.24.517575", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:42:25.765Z", "modified": "2026-08-20T10:42:25.915Z"}, {"entity": "publication", "iuid": "074bd7a0fee041f994069feb93fa3ecf", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/074bd7a0fee041f994069feb93fa3ecf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/074bd7a0fee041f994069feb93fa3ecf"}}, "title": "Epithelium-autonomous NAIP/NLRC4 prevents TNF-driven inflammatory destruction of the gut epithelial barrier in Salmonella-infected mice.", "authors": [{"family": "Fattinger", "given": "Stefan A", "initials": "SA", "orcid": "0000-0003-4899-4414", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a3891756a0e46b7be8494556da9254f.json"}}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Samperio Ventayol", "given": "Pilar", "initials": "P", "orcid": "0000-0002-3726-7871", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e7647686922142a8b8bd8629142445fc.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Furter", "given": "Markus", "initials": "M", "orcid": "0000-0003-4073-2268", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51f09daa062347adb84d145a0128c608.json"}}, {"family": "Felmy", "given": "Boas", "initials": "B"}, {"family": "Bakkeren", "given": "Erik", "initials": "E", "orcid": "0000-0001-7970-7890", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/374cea304e9d49129f59d95dfe72b72e.json"}}, {"family": "Hausmann", "given": "Annika", "initials": "A", "orcid": "0000-0001-8852-3444", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e0048a8661d44d5e929f1724cded7b39.json"}}, {"family": "Barthel-Scherrer", "given": "Manja", "initials": "M"}, {"family": "G\u00fcl", "given": "Ersin", "initials": "E"}, {"family": "Hardt", "given": "Wolf-Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "journal article", "published": "2021-05-00", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "volume": "14", "issue": "3", "pages": "615-629", "issn-l": "1933-0219"}, "abstract": "The gut epithelium is a critical protective barrier. Its NAIP/NLRC4 inflammasome senses infection by Gram-negative bacteria, including Salmonella Typhimurium (S.Tm) and promotes expulsion of infected enterocytes. During the first ~12-24 h, this reduces mucosal S.Tm loads at the price of moderate enteropathy. It remained unknown how this NAIP/NLRC4-dependent tradeoff would develop during subsequent infection stages. In NAIP/NLRC4-deficient mice, S.Tm elicited severe enteropathy within 72 h, characterized by elevated mucosal TNF (>20 pg/mg) production from bone marrow-derived cells, reduced regeneration, excessive enterocyte loss, and a collapse of the epithelial barrier. TNF-depleting antibodies prevented this destructive pathology. In hosts proficient for epithelial NAIP/NLRC4, a heterogeneous enterocyte death response with both apoptotic and pyroptotic features kept S.Tm loads persistently in check, thereby preventing this dire outcome altogether. Our results demonstrate that immediate and selective removal of infected enterocytes, by locally acting epithelium-autonomous NAIP/NLRC4, is required to avoid a TNF-driven inflammatory hyper-reaction that otherwise destroys the epithelial barrier.", "doi": "10.1038/s41385-021-00381-y", "pmid": "33731826", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "10.1038/s41385-021-00381-y"}, {"db": "pmc", "key": "PMC8075861"}], "notes": [], "created": "2021-12-05T09:46:52.963Z", "modified": "2022-11-04T11:32:13.344Z"}, {"entity": "publication", "iuid": "c1f8cf6dd77e4c37bf3981a01d459948", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c1f8cf6dd77e4c37bf3981a01d459948.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c1f8cf6dd77e4c37bf3981a01d459948"}}, "title": "Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers.", "authors": [{"family": "Samperio Ventayol", "given": "Pilar", "initials": "P", "orcid": "0000-0002-3726-7871", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e7647686922142a8b8bd8629142445fc.json"}}, {"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Florbrant", "given": "Alexandra", "initials": "A", "orcid": "0000-0002-1630-4442", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21b09621be1d4fe5a3c956360234b99d.json"}}, {"family": "Fattinger", "given": "Stefan A", "initials": "SA", "orcid": "0000-0003-4899-4414", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a3891756a0e46b7be8494556da9254f.json"}}, {"family": "Walder", "given": "Naemi", "initials": "N", "orcid": "0000-0002-2155-8527", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3f7de8ba54dc49568357b6f5c69681a5.json"}}, {"family": "Sima", "given": "Eduardo", "initials": "E", "orcid": "0000-0001-7373-4767", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a9855115aad340e3a1b9ecf05afb3b9d.json"}}, {"family": "Shao", "given": "Feng", "initials": "F", "orcid": "0000-0002-9562-7791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e64e1601b52746f5b81e2331a9bc58ab.json"}}, {"family": "Gekara", "given": "Nelson O", "initials": "NO", "orcid": "0000-0002-1269-8288", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/50781c8782ed4c78a8c993a97bef9661.json"}}, {"family": "Sundbom", "given": "Magnus", "initials": "M", "orcid": "0000-0002-6243-2859", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dec44b4c4ce84ce6b04bf9506874246b.json"}}, {"family": "Hardt", "given": "Wolf-Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}, {"family": "Webb", "given": "Dominic-Luc", "initials": "DL", "orcid": "0000-0002-6979-9194", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f8caab45e535418aa431bc55eca471e8.json"}}, {"family": "Hellstr\u00f6m", "given": "Per M", "initials": "PM", "orcid": "0000-0001-8428-0772", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9627ff4daff4db2802135e0d06282e0.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "journal article", "published": "2021-04-20", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "118", "issue": "16", "issn-l": "0027-8424"}, "abstract": "The gut epithelium serves to maximize the surface for nutrient and fluid uptake, but at the same time must provide a tight barrier to pathogens and remove damaged intestinal epithelial cells (IECs) without jeopardizing barrier integrity. How the epithelium coordinates these tasks remains a question of significant interest. We used imaging and an optical flow analysis pipeline to study the dynamicity of untransformed murine and human intestinal epithelia, cultured atop flexible hydrogel supports. Infection with the pathogen Salmonella Typhimurium (STm) within minutes elicited focal contractions with inward movements of up to \u223c1,000 IECs. Genetics approaches and chimeric epithelial monolayers revealed contractions to be triggered by the NAIP/NLRC4 inflammasome, which sensed type-III secretion system and flagellar ligands upon bacterial invasion, converting the local tissue into a contraction epicenter. Execution of the response required swift sublytic Gasdermin D pore formation, ion fluxes, and the propagation of a myosin contraction pulse across the tissue. Importantly, focal contractions preceded, and could be uncoupled from, the death and expulsion of infected IECs. In both two-dimensional monolayers and three-dimensional enteroids, multiple infection-elicited contractions coalesced to produce shrinkage of the epithelium as a whole. Monolayers deficient for Caspase-1(-11) or Gasdermin D failed to elicit focal contractions but were still capable of infected IEC death and expulsion. Strikingly, these monolayers lost their integrity to a markedly higher extent than wild-type counterparts. We propose that prompt NAIP/NLRC4/Caspase-1/Gasdermin D/myosin-dependent contractions allow the epithelium to densify its cell packing in infected regions, thereby preventing tissue disintegration due to the subsequent IEC death and expulsion process.", "doi": "10.1073/pnas.2013963118", "pmid": "33846244", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "2013963118"}, {"db": "pmc", "key": "PMC8072224"}], "notes": [], "created": "2021-12-05T09:46:51.321Z", "modified": "2022-11-04T11:32:13.392Z"}, {"entity": "publication", "iuid": "a088c580747242cc9cfc7c25f2b79601", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a088c580747242cc9cfc7c25f2b79601.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a088c580747242cc9cfc7c25f2b79601"}}, "title": "Salmonella enterica Serovar Typhimurium Exploits Cycling through Epithelial Cells To Colonize Human and Murine Enteroids.", "authors": [{"family": "Geiser", "given": "Petra", "initials": "P", "orcid": "0000-0003-2785-4201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0487e44d013c4faea6523a9f4b23cbe6.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Samperio Ventayol", "given": "Pilar", "initials": "P", "orcid": "0000-0002-3726-7871", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e7647686922142a8b8bd8629142445fc.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Sima", "given": "Eduardo", "initials": "E", "orcid": "0000-0001-7373-4767", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a9855115aad340e3a1b9ecf05afb3b9d.json"}}, {"family": "Al-Saffar", "given": "Anas Kh", "initials": "AK", "orcid": "0000-0002-4842-3227", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6fbadd286ab040e0a6f1a045b60aa52a.json"}}, {"family": "Ahl", "given": "David", "initials": "D", "orcid": "0000-0003-4364-8571", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/17570499e2b64bd697c4beaf9c679c07.json"}}, {"family": "Phillipson", "given": "Mia", "initials": "M", "orcid": "0000-0002-2387-0266", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/265c9b3e11de4fcaa270a0eb20faf65c.json"}}, {"family": "Webb", "given": "Dominic-Luc", "initials": "DL", "orcid": "0000-0002-6979-9194", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f8caab45e535418aa431bc55eca471e8.json"}}, {"family": "Sundbom", "given": "Magnus", "initials": "M", "orcid": "0000-0002-6243-2859", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dec44b4c4ce84ce6b04bf9506874246b.json"}}, {"family": "Hellstr\u00f6m", "given": "Per M", "initials": "PM", "orcid": "0000-0001-8428-0772", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9627ff4daff4db2802135e0d06282e0.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "journal article", "published": "2021-01-12", "journal": {"title": "MBio", "issn": "2150-7511", "volume": "12", "issue": "1", "issn-l": null}, "abstract": "Enterobacterial pathogens infect the gut by a multistep process, resulting in colonization of both the lumen and the mucosal epithelium. Due to experimental constraints, it remains challenging to address how luminal and epithelium-lodged pathogen populations cross-feed each other in vivo Enteroids are cultured three-dimensional miniature intestinal organs with a single layer of primary intestinal epithelial cells (IECs) surrounding a central lumen. They offer new opportunities to study enterobacterial infection under near-physiological conditions, at a temporal and spatial resolution not attainable in animal models, but remain poorly explored in this context. We employed microinjection, time-lapse microscopy, bacterial genetics, and barcoded consortium infections to describe the complete infection cycle of Salmonella enterica serovar Typhimurium in both human and murine enteroids. Flagellar motility and type III secretion system 1 (TTSS-1) promoted Salmonella Typhimurium targeting of the intraepithelial compartment and breaching of the epithelial barrier. Strikingly, however, TTSS-1 also potently boosted colonization of the enteroid lumen. By tracing the infection over time, we identified a cycle(s) of TTSS-1-driven IEC invasion, intraepithelial replication, and reemergence through infected IEC expulsion as a key mechanism for Salmonella Typhimurium luminal colonization. These findings suggest a positive feed-forward loop, through which IEC invasion by planktonic bacteria fuels further luminal population expansion, thereby ensuring efficient colonization of both the intraepithelial and luminal niches.IMPORTANCE Pathogenic gut bacteria are common causes of intestinal disease. Enteroids-cultured three-dimensional replicas of the mammalian gut-offer an emerging model system to study disease mechanisms under conditions that recapitulate key features of the intestinal tract. In this study, we describe the full life cycle of the prototype gut pathogen Salmonella enterica serovar Typhimurium within human and mouse enteroids. We map the consecutive steps and define the bacterial virulence factors that drive colonization of luminal and epithelial compartments, as well as breaching of the epithelial barrier. Strikingly, our work reveals how bacterial colonization of the epithelium potently fuels expansion also in the luminal compartment, through a mechanism involving the death and expulsion of bacterium-infected epithelial cells. These findings have repercussions for our understanding of the Salmonella infection cycle. Moreover, our work provides a comprehensive foundation for the use of microinjected enteroids to model gut bacterial diseases.", "doi": "10.1128/mBio.02684-20", "pmid": "33436434", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7844539"}, {"db": "pii", "key": "mBio.02684-20"}], "notes": [], "created": "2021-12-05T09:46:54.328Z", "modified": "2026-08-21T09:30:27.020Z"}]}