{"entity": "researcher", "timestamp": "2026-08-28T00:26:22.999Z", "family": "Madugalle", "given": "Sachithrani U", "initials": "SU", "orcid": "0000-0001-6606-8196", "affiliations": ["Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/024dbfb8cc85447cad7373fce4a5a7ce.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/024dbfb8cc85447cad7373fce4a5a7ce"}}, "publications": [{"entity": "publication", "iuid": "5c1fbe1837b64c0c870e68e9e5480a0a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5c1fbe1837b64c0c870e68e9e5480a0a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5c1fbe1837b64c0c870e68e9e5480a0a"}}, "title": "Isoform-level profiling of m6A epitranscriptomic signatures in human brain.", "authors": [{"family": "Gleeson", "given": "Josie", "initials": "J", "orcid": "0000-0002-4302-9191", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/706aa682a02c45d1b31eb10012a53953.json"}}, {"family": "Madugalle", "given": "Sachithrani U", "initials": "SU", "orcid": "0000-0001-6606-8196", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/024dbfb8cc85447cad7373fce4a5a7ce.json"}}, {"family": "Wan", "given": "Ching Yin", "initials": "CY", "orcid": "0009-0003-0571-9102", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/98df0b264ecd4de4b9c4c5ca7da53348.json"}}, {"family": "McLean", "given": "Catriona", "initials": "C", "orcid": "0000-0002-0302-5727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be3d4552d75742b6869f3fff10f54f67.json"}}, {"family": "Bredy", "given": "Timothy W", "initials": "TW", "orcid": "0000-0003-3280-126X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21e040c297234b2b968a55c97af6efe2.json"}}, {"family": "De Paoli-Iseppi", "given": "Ricardo", "initials": "R", "orcid": "0000-0001-7724-9144", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a8b865814ba48edbed55cbcbe575395.json"}}, {"family": "Clark", "given": "Michael B", "initials": "MB", "orcid": "0000-0002-2903-9537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d459e6148fae4b1292f9bcbd384ea42d.json"}}], "type": "journal article", "published": "2025-08-08", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "11", "issue": "32", "pages": "eadp0783", "issn-l": "2375-2548"}, "abstract": "The RNA modification N6-methyladenosine (m6A) is highly abundant in human brain and implicated in neurological disorders. Profiling m6A within RNA isoforms is a critical step toward understanding the complex mechanisms that underpin brain function and disease; however, we lack an isoform-level atlas of m6A sites in the brain. We applied Oxford Nanopore direct RNA sequencing (DRS) to three postmortem human brain regions-prefrontal cortex, caudate nucleus, and cerebellum-to simultaneously investigate the transcriptome and epitranscriptome at the isoform level. We identified 57,000 m6A sites within 15,000 isoforms, revealing both isoform- and brain region-specific patterning of m6A modifications. The prefrontal cortex exhibited a distinctive profile of specifically modified isoforms enriched in excitatory neurons and had the highest proportion of unannotated m6A sites. A population of isoforms were hypermodified and associated with excitatory neurons in all brain regions. Our results demonstrate the utility of isoform-level profiling of RNA modifications and provide insights into brain region specificity with implications for development and disease.", "doi": "10.1126/sciadv.adp0783", "pmid": "40779635", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12333690"}], "notes": [], "created": "2026-08-21T12:22:31.579Z", "modified": "2026-08-21T12:22:31.880Z"}]}