{"entity": "researcher", "timestamp": "2026-05-12T22:08:26.689Z", "family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "affiliations": ["Science for Life Laboratory, Department of Environmental Science, Stockholm University, 114 18 Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00"}}, "publications": [{"entity": "publication", "iuid": "0af8655f8f7141309ddf390ea2e02967", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0af8655f8f7141309ddf390ea2e02967.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0af8655f8f7141309ddf390ea2e02967"}}, "title": "Nontarget Analysis of Polluted Surface Waters in Bangladesh Using Open Science Workflows.", "authors": [{"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Karlsson", "given": "Oskar", "initials": "O", "orcid": "0000-0001-8009-0015", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9e8bc211d3eb432a877e1f32671e6aa8.json"}}, {"family": "Rian", "given": "May Britt", "initials": "MB", "orcid": "0000-0001-6153-2164", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/191d5d1c23714beb95be36a7a9140da6.json"}}, {"family": "Raqib", "given": "Rubhana", "initials": "R"}, {"family": "Parvez", "given": "Faruque", "initials": "F"}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "Islam", "given": "M Sirajul", "initials": "MS"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}], "type": "journal article", "published": "2023-05-02", "journal": {"title": "Environ. Sci. Technol.", "issn": "1520-5851", "volume": "57", "issue": "17", "pages": "6808-6824", "issn-l": "0013-936X"}, "abstract": "Nontarget mass spectrometry has great potential to reveal patterns of water contamination globally through community science, but few studies are conducted in low-income countries, nor with open-source workflows, and few datasets are FAIR (Findable, Accessible, Interoperable, Reusable). Water was collected from urban and rural rivers around Dhaka, Bangladesh, and analyzed by liquid chromatography high-resolution mass spectrometry in four ionization modes (electrospray ionization \u00b1, atmospheric pressure chemical ionization \u00b1) with data-independent MS2 acquisition. The acquisition strategy was complementary: 19,427 and 7365 features were unique to ESI and APCI, respectively. The complexity of water pollution was revealed by >26,000 unique molecular features resolved by MS-DIAL, among which >20,000 correlated with urban sources in Dhaka. A major wastewater treatment plant was not a dominant pollution source, consistent with major contributions from uncontrolled urban drainage, a result that encourages development of further wastewater infrastructures. Matching of deconvoluted MS2 spectra to public libraries resulted in 62 confident annotations (i.e., Level 1-2a) and allowed semiquantification of 42 analytes including pharmaceuticals, pesticides, and personal care products. In silico structure prediction for the top 100 unknown molecular features associated with an urban source allowed 15 additional chemicals of anthropogenic origin to be annotated (i.e., Level 3). The authentic MS2 spectra were uploaded to MassBank Europe, mass spectral data were openly shared on the MassIVE repository, a tool (i.e., MASST) that could be used for community science environmental surveillance was demonstrated, and current limitations were discussed.", "doi": "10.1021/acs.est.2c08200", "pmid": "37083417", "labels": {"SciLifeLab Fellow": null, "Oskar Karlsson": null}, "xrefs": [{"db": "pmc", "key": "PMC10157886"}], "notes": [], "created": "2023-06-10T20:04:33.700Z", "modified": "2023-06-10T20:04:33.835Z"}, {"entity": "publication", "iuid": "f71901872b0e48c6bd0af44f2c094ba9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f71901872b0e48c6bd0af44f2c094ba9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f71901872b0e48c6bd0af44f2c094ba9"}}, "title": "Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies.", "authors": [{"family": "K\u00e4llsten", "given": "Liselott", "initials": "L", "orcid": "0000-0002-1889-196X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c399772491e84746993efbaaa75acdc5.json"}}, {"family": "Pierozan", "given": "Paula", "initials": "P"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}, {"family": "Karlsson", "given": "Oskar", "initials": "O"}], "type": "journal article", "published": "2022-09-27", "journal": {"title": "Cells", "issn": "2073-4409", "volume": "11", "issue": "19", "issn-l": "2073-4409"}, "abstract": "The widespread environmental contaminant di-n-butyl phthalate (DBP) has been linked with reduced testosterone levels and adverse reproductive health outcomes in men. However, the underlying mechanisms of these anti-androgenic effects and the potential effects on other classes of steroid hormones remain to be elucidated. Here, we conducted mechanistic studies in human adrenocortical H295R cells exposed to 1-500 \u00b5M of DBP or its metabolite, mono-n-butyl phthalate (MBP), for 48 h. Quantification of steroid hormones in the cell medium by liquid chromatography-mass spectrometry revealed that both phthalates significantly decreased testosterone, androstenedione, corticosterone, and progesterone levels, in particular after dibutyryl-cyclic-AMP stimulation of steroidogenesis. Western blot analysis of key steroidogenic proteins showed that DBP induced a dose-dependent decrease of CYP11A1 and HSD3\u03b22 levels, while MBP only significantly decreased CYP17A1 levels, indicating that the compounds affect early steps of the steroidogenesis differently. Both DBP and MBP exposure also lead to a dose-related decrease in HSD17\u03b23, the enzyme which catalyzes the final step in the testosterone biosynthesis pathway, although these effects were not statistically significant. Interestingly, DBP increased the cortisol concentration, which may be due to the non-significant CYP11B1 increase in DBP-exposed cells. In contrast, MBP decreased cortisol concentration. Moreover, the analysis of superoxide generation and quantification of the protein oxidation marker nitrotyrosine demonstrated that DBP induced oxidative stress in H295R cells while MBP reduced protein nitrotyrosine levels. These findings confirm the anti-androgenic effects of DBP and MBP and reveal several differences in their toxicological mechanisms, with possible implications for future research on phthalate toxicity.", "doi": "10.3390/cells11193029", "pmid": "36230992", "labels": {"SciLifeLab Fellow": null, "Oskar Karlsson": null}, "xrefs": [{"db": "pmc", "key": "PMC9561974"}, {"db": "pii", "key": "cells11193029"}], "notes": [], "created": "2023-06-10T19:59:18.419Z", "modified": "2023-06-10T19:59:18.850Z"}]}