{"entity": "publication", "iuid": "fce859c435a94f7e84ec6c34991ef0b4", "timestamp": "2026-08-23T10:13:48.062Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fce859c435a94f7e84ec6c34991ef0b4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fce859c435a94f7e84ec6c34991ef0b4"}}, "title": "Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics.", "authors": [{"family": "Faridi", "given": "Muhammad Asim", "initials": "MA"}, {"family": "Ramachandraiah", "given": "Harisha", "initials": "H"}, {"family": "Banerjee", "given": "Indradumna", "initials": "I"}, {"family": "Ardabili", "given": "Sahar", "initials": "S"}, {"family": "Zelenin", "given": "Sergey", "initials": "S"}, {"family": "Russom", "given": "Aman", "initials": "A", "orcid": "0000-0002-0242-358X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8939625551d5409aac4593329e2a6578.json"}}], "type": "journal article", "published": "2017-01-04", "journal": {"title": "J Nanobiotechnology", "issn": "1477-3155", "volume": "15", "issue": "1", "pages": "3", "issn-l": null}, "abstract": "Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics.\n\nIn elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non-Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be separated. We first optimized the flow conditions using particles, and show continuous separation of 5 \u03bcm particles from 2 \u03bcm at a yield of 95% for 5 \u00b5m particle and 93% for 2 \u00b5m particles at respective outlets. Next, bacteria were continuously separated at an efficiency of 76% from undiluted whole blood sample.\n\nWe demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria.", "doi": "10.1186/s12951-016-0235-4", "pmid": "28052769", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5210221"}, {"db": "pii", "key": "10.1186/s12951-016-0235-4"}], "notes": [], "created": "2026-08-20T12:20:45.098Z", "modified": "2026-08-20T12:20:45.199Z"}