{"entity": "publication", "iuid": "fa922a521fd2428b9f746a316907a579", "timestamp": "2026-09-23T15:36:14.115Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fa922a521fd2428b9f746a316907a579.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fa922a521fd2428b9f746a316907a579"}}, "title": "Plasma proteins elevated in severe asthma despite oral steroid use and unrelated to Type-2 inflammation.", "authors": [{"family": "Sparreman Mikus", "given": "Maria", "initials": "M"}, {"family": "Kolmert", "given": "Johan", "initials": "J"}, {"family": "Andersson", "given": "Lars I", "initials": "LI"}, {"family": "\u00d6stling", "given": "J\u00f6rgen", "initials": "J"}, {"family": "Knowles", "given": "Richard G", "initials": "RG"}, {"family": "G\u00f3mez", "given": "Cristina", "initials": "C", "orcid": "0000-0001-8518-0095", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fa2cfd19e5e1481f9917ac73c00f0934.json"}}, {"family": "Ericsson", "given": "Magnus", "initials": "M"}, {"family": "Th\u00f6rngren", "given": "John-Olof", "initials": "JO"}, {"family": "Emami Khoonsari", "given": "Payam", "initials": "P"}, {"family": "Dahl\u00e9n", "given": "Barbro", "initials": "B"}, {"family": "Kupczyk", "given": "Maciej", "initials": "M"}, {"family": "De Meulder", "given": "Bertrand", "initials": "B"}, {"family": "Auffray", "given": "Charles", "initials": "C"}, {"family": "Bakke", "given": "Per S", "initials": "PS"}, {"family": "Beghe", "given": "Bianca", "initials": "B"}, {"family": "Bel", "given": "Elisabeth H", "initials": "EH"}, {"family": "Caruso", "given": "Massimo", "initials": "M", "orcid": "0000-0002-4412-2080", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ef254e4ff45d4919a6ca0922bb6be1ac.json"}}, {"family": "Chanez", "given": "Pascal", "initials": "P"}, {"family": "Chawes", "given": "Bo", "initials": "B"}, {"family": "Fowler", "given": "Stephen J", "initials": "SJ", "orcid": "0000-0002-4524-1663", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/017b3977b2a245a4844c65f6e2bc9038.json"}}, {"family": "Gaga", "given": "Mina", "initials": "M", "orcid": "0000-0002-9949-6012", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d145e5de25554c61b2fda8de58af13cf.json"}}, {"family": "Geiser", "given": "Thomas", "initials": "T"}, {"family": "Gjomarkaj", "given": "Mark", "initials": "M"}, {"family": "Horv\u00e1th", "given": "Ildik\u00f3", "initials": "I"}, {"family": "Howarth", "given": "Peter H", "initials": "PH", "orcid": "0000-0003-0619-7927", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bec1e3ab30dc4d3d81479092aa59d818.json"}}, {"family": "Johnston", "given": "Sebastian L", "initials": "SL"}, {"family": "Joos", "given": "Guy", "initials": "G", "orcid": "0000-0002-3149-9444", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/153de2cdf539481bab956ec616211575.json"}}, {"family": "Krug", "given": "Norbert", "initials": "N"}, {"family": "Montuschi", "given": "Paolo", "initials": "P"}, {"family": "Musial", "given": "Jacek", "initials": "J"}, {"family": "Ni\u017cankowska-Mogilnicka", "given": "Ewa", "initials": "E"}, {"family": "Olsson", "given": "Henric K", "initials": "HK"}, {"family": "Papi", "given": "Alberto", "initials": "A"}, {"family": "Rabe", "given": "Klaus F", "initials": "KF"}, {"family": "Sandstr\u00f6m", "given": "Thomas", "initials": "T"}, {"family": "Shaw", "given": "Dominick E", "initials": "DE", "orcid": "0000-0003-4106-8469", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a2bf9d665f224457ab014cc6d936a127.json"}}, {"family": "Siafakas", "given": "Nikolaos M", "initials": "NM"}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M"}, {"family": "Riley", "given": "John H", "initials": "JH"}, {"family": "Bates", "given": "Stewart", "initials": "S"}, {"family": "Middelveld", "given": "Roelinde J M", "initials": "RJM"}, {"family": "Wheelock", "given": "Craig E", "initials": "CE", "orcid": "0000-0002-8113-0653", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d98abddc2b14a35ade5cb6d6d2f540d.json"}}, {"family": "Chung", "given": "Kian Fan", "initials": "KF"}, {"family": "Adcock", "given": "Ian M", "initials": "IM", "orcid": "0000-0003-2101-8843", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b61bb5380bf419c8e5ce461e36b7f7c.json"}}, {"family": "Sterk", "given": "Peter J", "initials": "PJ"}, {"family": "Djukanovic", "given": "Ratko", "initials": "R"}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3db33fa3edb94febb8be7927a16838d0.json"}}, {"family": "Dahl\u00e9n", "given": "Sven-Erik", "initials": "SE"}, {"family": "James", "given": "Anna", "initials": "A"}, {"family": "U-BIOPRED (Unbiased Biomarkers for the Prediction of Respiratory Disease outcome) Study Group and the BIOAIR (Longitudinal Assessment of Clinical Course and Biomarkers in Severe Chronic Airway Disease) Consortium", "given": "", "initials": ""}], "type": "controlled clinical trial", "published": "2022-02-00", "journal": {"title": "Eur. Respir. J.", "issn": "1399-3003", "volume": "59", "issue": "2", "issn-l": "0903-1936"}, "abstract": "Asthma phenotyping requires novel biomarker discovery.\n\nTo identify plasma biomarkers associated with asthma phenotypes by application of a new proteomic panel to samples from two well-characterised cohorts of severe (SA) and mild-to-moderate (MMA) asthmatics, COPD subjects and healthy controls (HCs).\n\nAn antibody-based array targeting 177 proteins predominantly involved in pathways relevant to inflammation, lipid metabolism, signal transduction and extracellular matrix was applied to plasma from 525 asthmatics and HCs in the U-BIOPRED cohort, and 142 subjects with asthma and COPD from the validation cohort BIOAIR. Effects of oral corticosteroids (OCS) were determined by a 2-week, placebo-controlled OCS trial in BIOAIR, and confirmed by relation to objective OCS measures in U-BIOPRED.\n\nIn U-BIOPRED, 110 proteins were significantly different, mostly elevated, in SA compared to MMA and HCs. 10 proteins were elevated in SA versus MMA in both U-BIOPRED and BIOAIR (alpha-1-antichymotrypsin, apolipoprotein-E, complement component 9, complement factor I, macrophage inflammatory protein-3, interleukin-6, sphingomyelin phosphodiesterase 3, TNF receptor superfamily member 11a, transforming growth factor-\u03b2 and glutathione S-transferase). OCS treatment decreased most proteins, yet differences between SA and MMA remained following correction for OCS use. Consensus clustering of U-BIOPRED protein data yielded six clusters associated with asthma control, quality of life, blood neutrophils, high-sensitivity C-reactive protein and body mass index, but not Type-2 inflammatory biomarkers. The mast cell specific enzyme carboxypeptidase A3 was one major contributor to cluster differentiation.\n\nThe plasma proteomic panel revealed previously unexplored yet potentially useful Type-2-independent biomarkers and validated several proteins with established involvement in the pathophysiology of SA.", "doi": "10.1183/13993003.00142-2021", "pmid": "34737220", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8850689"}, {"db": "pii", "key": "13993003.00142-2021"}], "notes": [], "created": "2026-09-23T09:54:30.140Z", "modified": "2026-09-23T10:28:41.711Z"}