{"entity": "publication", "iuid": "f9e50483c8894b8997cdc31a8662556d", "timestamp": "2026-09-25T23:04:24.839Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f9e50483c8894b8997cdc31a8662556d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f9e50483c8894b8997cdc31a8662556d"}}, "title": "Using RNAseq to Uncover Transcriptional and Splicing Differences in Host Cells During Rift Valley Fever Virus Infection.", "authors": [{"family": "White", "given": "Luke Adam", "initials": "LA"}, {"family": "Havranek", "given": "Katherine E", "initials": "KE"}, {"family": "Lodmell", "given": "J Stephen", "initials": "JS"}], "type": "journal article", "published": "2024-07-23", "journal": {"title": "Methods in molecular biology (Clifton, N.J.)", "issn": "1940-6029", "volume": "2824", "pages": "373-383", "issn-l": "1064-3745"}, "abstract": "RNAseq is a valuable tool that can aid researchers in uncovering the transcriptional changes that occur when a viral pathogen infects a host cell. Viral infection will invariably cause differential expression of many genes, from transcription of mRNA to alternative splicing and degradation. This change in gene expression can be a result of immune activation or a direct activity of the virus to alter the host cell's environment to make it more favorable for viral replication. Studying the innate immune response to a pathogen can reveal which cellular pathways are active, indicating the steps that the host takes to halt viral infection, and detecting virus-mediated mRNA expression changes can help with identifying the pathways which may be exploited by the virus. Gene expression changes-both cell-caused and virus-caused-can be studied through RNAseq, helping to provide a clearer picture of the cellular changes that occur during viral infection. In this protocol, we outline methods to carry out mRNA sequencing in Rift Valley fever virus-infected cell cultures, from infection to library prep and analysis.", "doi": "10.1007/978-1-0716-3926-9_23", "pmid": "39039424", "labels": [], "xrefs": [{"db": "mid", "key": "NIHMS2177357"}, {"db": "pmc", "key": "PMC13283058"}], "notes": [], "created": "2026-09-23T15:39:59.936Z", "modified": "2026-09-23T15:39:59.968Z"}