{"entity": "publication", "iuid": "f6e51891bd874ff584a722df2afb244a", "timestamp": "2026-08-26T22:46:49.510Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f6e51891bd874ff584a722df2afb244a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f6e51891bd874ff584a722df2afb244a"}}, "title": "Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics.", "authors": [{"family": "Herr", "given": "Patrick", "initials": "P"}, {"family": "Bostr\u00f6m", "given": "Johan", "initials": "J"}, {"family": "Rullman", "given": "Eric", "initials": "E"}, {"family": "Rudd", "given": "Sean G", "initials": "SG"}, {"family": "Vesterlund", "given": "Mattias", "initials": "M"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Helleday", "given": "Thomas", "initials": "T"}, {"family": "Maddalo", "given": "Gianluca", "initials": "G"}, {"family": "Altun", "given": "Mikael", "initials": "M"}], "type": "journal article", "published": "2020-04-00", "journal": {"title": "Mol. Cell Proteomics", "issn": "1535-9484", "volume": "19", "issue": "4", "pages": "608-623", "issn-l": "1535-9476"}, "abstract": "The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating proteins, as exemplified by the S-adenosylmethionine synthase MAT2A. Our study implicates this enzyme in translocating to the nucleus after the G1/S-checkpoint, which enables epigenetic histone methylation maintenance during DNA replication. Taken together, this data set provides a unique integrated resource with novel insights on cell cycle dynamics.", "doi": "10.1074/mcp.RA120.001938", "pmid": "32051232", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7124475"}, {"db": "pii", "key": "S1535-9476(20)35020-9"}], "notes": [], "created": "2026-08-20T09:32:48.815Z", "modified": "2026-08-21T09:27:51.208Z"}