{"entity": "publication", "iuid": "f5814a41c2ab491dbc483f32d52b436c", "timestamp": "2026-08-20T21:26:17.245Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f5814a41c2ab491dbc483f32d52b436c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f5814a41c2ab491dbc483f32d52b436c"}}, "title": "Resolution of ribosomal stalling by EF-P and ABCF ATPases YfmR and YkpA/YbiT.", "authors": [{"family": "Takada", "given": "Hiraku", "initials": "H", "orcid": "0000-0003-2034-5666", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/92f74a1a0bb543ed8504ed034144b2bc.json"}}, {"family": "Fujiwara", "given": "Keigo", "initials": "K"}, {"family": "Atkinson", "given": "Gemma C", "initials": "GC"}, {"family": "Chiba", "given": "Shinobu", "initials": "S", "orcid": "0000-0003-3485-3477", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c5d57c159f4c44f3ad179504c9558880.json"}}, {"family": "Hauryliuk", "given": "Vasili", "initials": "V", "orcid": "0000-0003-2389-5057", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1884d638ebd34b34bcabc105ca2f4092.json"}}], "type": "journal article", "published": "2024-09-09", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "52", "issue": "16", "pages": "9854-9866", "issn-l": "0305-1048"}, "abstract": "Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid composition of the assembled amino acid chain. Challenging sequences include proline-rich motifs as well as highly positively and negatively charged amino acid stretches. Members of the F subfamily of ABC ATPases (ABCFs) have been long hypothesised to promote translation of such problematic motifs. In this study we have applied genetics and reporter-based assays to characterise the four housekeeping ABCF ATPases of Bacillus subtilis: YdiF, YfmM, YfmR/Uup and YkpA/YbiT. We show that YfmR cooperates with the translation factor EF-P that promotes translation of Pro-rich motifs. Simultaneous loss of both YfmR and EF-P results in a dramatic growth defect. Surprisingly, this growth defect can be largely suppressed though overexpression of an EF-P variant lacking the otherwise crucial 5-amino-pentanolylated residue K32. Using in vivo reporter assays, we show that overexpression of YfmR can alleviate ribosomal stalling on Asp-Pro motifs. Finally, we demonstrate that YkpA/YbiT promotes translation of positively and negatively charged motifs but is inactive in resolving ribosomal stalls on proline-rich stretches. Collectively, our results provide insights into the function of ABCF translation factors in modulating protein synthesis in B. subtilis.", "doi": "10.1093/nar/gkae556", "pmid": "38943426", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11381351"}, {"db": "pii", "key": "7701496"}], "notes": [], "created": "2026-08-20T09:50:08.728Z", "modified": "2026-08-20T09:50:08.824Z"}