{"entity": "publication", "iuid": "f2728c181f384dfca89f398be5c9cf39", "timestamp": "2026-08-22T07:48:34.157Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f2728c181f384dfca89f398be5c9cf39.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f2728c181f384dfca89f398be5c9cf39"}}, "title": "High resolution and rapid separation of bacteria from blood using elasto-inertial microfluidics.", "authors": [{"family": "Narayana Iyengar", "given": "Sharath", "initials": "S", "orcid": "0000-0001-5348-3526", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3ab95c0e76c402c85016a02822b247b.json"}}, {"family": "Kumar", "given": "Tharagan", "initials": "T"}, {"family": "M\u00e5rtensson", "given": "Gustaf", "initials": "G"}, {"family": "Russom", "given": "Aman", "initials": "A", "orcid": "0000-0002-0242-358X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8939625551d5409aac4593329e2a6578.json"}}], "type": "journal article", "published": "2021-12-00", "journal": {"title": "Electrophoresis", "issn": "1522-2683", "volume": "42", "issue": "23", "pages": "2538-2551", "issn-l": "0173-0835"}, "abstract": "Improved sample preparation has the potential to address unmet needs for fast turnaround sepsis tests. In this work, we report elasto-inertial based rapid bacteria separation from diluted blood at high separation efficiency. In viscoelastic flows, we demonstrate novel findings where blood cells prepositioned at the outer wall entering a spiral device remain fully focused throughout the channel length while smaller bacteria migrate to the opposite wall. Initially, using microparticles, we show that particles above a certain size cut-off remain fully focused at the outer wall while smaller particles differentially migrate toward the inner wall. We demonstrate particle separation at 1 \u03bcm resolution at a total throughput of 1 mL/min. For blood-based experiments, a minimum of 1:2 dilution was necessary to fully focus blood cells at the outer wall. Finally, Escherichia coli spiked in diluted blood were continuously separated at a total flow rate of 1 mL/min, with efficiencies between 82 and 90% depending on the blood dilution. Using a single spiral, it takes 40 min to process 1 mL of blood at a separation efficiency of 82%. The label-free, passive, and rapid bacteria isolation method has a great potential for speeding up downstream phenotypic and genotypic analysis.", "doi": "10.1002/elps.202100140", "pmid": "34510466", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:32:42.679Z", "modified": "2026-08-20T06:32:42.795Z"}