{"entity": "publication", "iuid": "f24af4391b6f44b09538e1d61f8e6eb7", "timestamp": "2026-10-05T01:23:18.053Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f24af4391b6f44b09538e1d61f8e6eb7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f24af4391b6f44b09538e1d61f8e6eb7"}}, "title": "An extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).", "authors": [{"family": "G\u00f6ransson", "given": "Sara", "initials": "S"}, {"family": "Chen", "given": "Shan", "initials": "S"}, {"family": "Olofsson", "given": "Helene", "initials": "H"}, {"family": "Larsson", "given": "Ola", "initials": "O"}, {"family": "Str\u00f6mblad", "given": "Staffan", "initials": "S"}], "type": "journal article", "published": "2023-04-30", "journal": {"title": "Biochem. Biophys. Res. Commun.", "issn": "1090-2104", "volume": "654", "pages": "73-79", "issn-l": "0006-291X"}, "abstract": "Identifying mechanisms driving the transition from ductal carcinoma in situ (DCIS) to invasive breast cancer remains a challenge in breast cancer research. Breast cancer progression is accompanied by remodelling and stiffening of the extracellular matrix, leading to increased proliferation, survival, and migration. Here, we studied stiffness-dependent phenotypes in MCF10CA1a (CA1a) breast cancer cells cultured on hydrogels with stiffness corresponding to normal breast and breast cancer. This revealed a stiffness-associated morphology consistent with acquisition of an invasive phenotype in breast cancer cells. Surprisingly, this strong phenotypic switch was accompanied by relatively modest transcriptome-wide alterations in mRNA levels, as independently quantified using both DNA-microarrays and bulk RNA sequencing. Strikingly, however, the stiffness-dependent alterations in mRNA levels overlapped with those contrasting ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC). This supports a role of matrix stiffness in driving the pre-invasive to invasive transition and suggests that mechanosignalling may be a target for prevention of invasive breast cancer.", "doi": "10.1016/j.bbrc.2023.03.001", "pmid": "36893606", "labels": [], "xrefs": [{"db": "pii", "key": "S0006-291X(23)00271-1"}], "notes": [], "created": "2026-09-23T11:19:37.977Z", "modified": "2026-09-23T11:19:38.011Z"}