{"entity": "publication", "iuid": "ee0bf1b568d94662810a58c0ce631880", "timestamp": "2026-09-25T23:04:35.484Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ee0bf1b568d94662810a58c0ce631880.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ee0bf1b568d94662810a58c0ce631880"}}, "title": "Application of FRET- and BRET-based live-cell biosensors in deorphanization and ligand discovery studies on orphan G protein-coupled receptors.", "authors": [{"family": "Sajkowska", "given": "Joanna J", "initials": "JJ"}, {"family": "Tsang", "given": "Choi Har", "initials": "CH"}, {"family": "Kozielewicz", "given": "Pawe\u0142", "initials": "P"}], "type": "journal article", "published": "2024-09-00", "journal": {"title": "SLAS Discov", "issn": "2472-5560", "volume": "29", "issue": "6", "pages": "100174", "issn-l": "2472-5552"}, "abstract": "Bioluminescence- and fluorescence-based resonance energy transfer assays have gained considerable attention in pharmacological research as high-throughput scalable tools applicable to drug discovery. To this end, G protein-coupled receptors represent the biggest target class for marketed drugs, and among them, orphan G protein-coupled receptors have the biggest untapped therapeutic potential. In this review, the cases where biophysical methods, BRET and FRET, were employed for deorphanization and ligand discovery studies on orphan G protein-coupled receptors are listed and discussed.", "doi": "10.1016/j.slasd.2024.100174", "pmid": "39084335", "labels": [], "xrefs": [{"db": "pii", "key": "S2472-5552(24)00036-4"}], "notes": [], "created": "2026-09-23T11:24:43.817Z", "modified": "2026-09-23T11:24:43.830Z"}