{"entity": "publication", "iuid": "eba66f25fbbc4b00985e7cace9394686", "timestamp": "2026-08-22T07:47:55.859Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/eba66f25fbbc4b00985e7cace9394686.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/eba66f25fbbc4b00985e7cace9394686"}}, "title": "In vivo engineering of murine T cells using the evolved adeno-associated virus variant Ark313.", "authors": [{"family": "Nyberg", "given": "William A", "initials": "WA"}, {"family": "Wang", "given": "Charlotte H", "initials": "CH"}, {"family": "Ark", "given": "Jonathan", "initials": "J"}, {"family": "Liu", "given": "Chang", "initials": "C"}, {"family": "Clouden", "given": "Sylvanie", "initials": "S"}, {"family": "Qualls", "given": "Anita", "initials": "A"}, {"family": "Caryotakis", "given": "Sofia", "initials": "S"}, {"family": "Wells", "given": "Elina", "initials": "E"}, {"family": "Simon", "given": "Katherine", "initials": "K"}, {"family": "Garza", "given": "Celeste", "initials": "C"}, {"family": "Bernard", "given": "Pierre-Louis", "initials": "PL"}, {"family": "Lopez-Ichikawa", "given": "Maya", "initials": "M"}, {"family": "Li", "given": "Zhongmei", "initials": "Z"}, {"family": "Seo", "given": "Jin", "initials": "J"}, {"family": "Kimmerly", "given": "Gabriella R", "initials": "GR"}, {"family": "Muldoon", "given": "Joseph J", "initials": "JJ"}, {"family": "Chen", "given": "Peixin Amy", "initials": "PA"}, {"family": "Li", "given": "Mingcheng", "initials": "M"}, {"family": "Liang", "given": "Hong-Erh", "initials": "HE"}, {"family": "Kersten", "given": "Kelly", "initials": "K"}, {"family": "Rosales", "given": "Alan", "initials": "A"}, {"family": "Kuhn", "given": "Nicholas", "initials": "N"}, {"family": "Ye", "given": "Chun Jimmie", "initials": "CJ"}, {"family": "Gardner", "given": "James M", "initials": "JM"}, {"family": "Molofsky", "given": "Ari", "initials": "A"}, {"family": "Ricardo-Gonzalez", "given": "Roberto R", "initials": "RR"}, {"family": "Asokan", "given": "Aravind", "initials": "A"}, {"family": "Eyquem", "given": "Justin", "initials": "J"}], "type": "journal article", "published": "2025-02-11", "journal": {"title": "Immunity", "issn": "1097-4180", "volume": "58", "issue": "2", "pages": "499-512.e7", "issn-l": null}, "abstract": "Genetic engineering of T cells in mouse models is essential for investigating immune mechanisms. We aimed to develop an approach to manipulate T cells in vivo using an evolved adeno-associated virus (AAV) capsid named Ark313. Delivery of a transient transgene expression cassette was feasible using Ark313, and this serotype outperformed natural serotypes. A single intravenous injection of a Cre recombinase-expressing Ark313 in the Ai9 fluorescent reporter mouse model achieved permanent genetic modifications of T cells. Ark313 facilitated in vivo gene editing in both tissue-resident and splenic T cells and validation of immunotherapy targets in solid tumor models. Ark313 delivered large DNA donor templates to T cells in vivo and integrated transgenes in primary CD4+ and CD8+ T cells, including naive T cells. Ark313-mediated transgene delivery presents an efficient approach to target mouse T cells in vivo and a resource for the interrogation of T cell biology and for immunotherapy applications.", "doi": "10.1016/j.immuni.2025.01.009", "pmid": "39909036", "labels": [], "xrefs": [{"db": "pii", "key": "S1074-7613(25)00034-2"}], "notes": [], "created": "2026-08-21T11:21:02.444Z", "modified": "2026-08-21T11:21:02.455Z"}