{"entity": "publication", "iuid": "e6c76d5375fc4386b774d41b4f8ea181", "timestamp": "2026-08-22T06:52:02.457Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e6c76d5375fc4386b774d41b4f8ea181.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e6c76d5375fc4386b774d41b4f8ea181"}}, "title": "Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults.", "authors": [{"family": "Holliday", "given": "Katelyn M", "initials": "KM"}, {"family": "Gondalia", "given": "Rahul", "initials": "R"}, {"family": "Baldassari", "given": "Antoine", "initials": "A"}, {"family": "Justice", "given": "Anne E", "initials": "AE"}, {"family": "Stewart", "given": "James D", "initials": "JD"}, {"family": "Liao", "given": "Duanping", "initials": "D"}, {"family": "Yanosky", "given": "Jeff D", "initials": "JD"}, {"family": "Jordahl", "given": "Kristina M", "initials": "KM"}, {"family": "Bhatti", "given": "Parveen", "initials": "P"}, {"family": "Assimes", "given": "Themistocles L", "initials": "TL"}, {"family": "Pankow", "given": "James S", "initials": "JS"}, {"family": "Guan", "given": "Weihua", "initials": "W"}, {"family": "Fornage", "given": "Myriam", "initials": "M"}, {"family": "Bressler", "given": "Jan", "initials": "J"}, {"family": "North", "given": "Kari E", "initials": "KE"}, {"family": "Conneely", "given": "Karen N", "initials": "KN"}, {"family": "Li", "given": "Yun", "initials": "Y"}, {"family": "Hou", "given": "Lifang", "initials": "L"}, {"family": "Vokonas", "given": "Pantel S", "initials": "PS"}, {"family": "Ward-Caviness", "given": "Cavin K", "initials": "CK"}, {"family": "Wilson", "given": "Rory", "initials": "R"}, {"family": "Wolf", "given": "Kathrin", "initials": "K"}, {"family": "Waldenberger", "given": "Melanie", "initials": "M"}, {"family": "Cyrys", "given": "Josef", "initials": "J"}, {"family": "Peters", "given": "Annette", "initials": "A"}, {"family": "Boezen", "given": "H Marike", "initials": "HM"}, {"family": "Vonk", "given": "Judith M", "initials": "JM"}, {"family": "Sayols-Baixeras", "given": "Sergi", "initials": "S"}, {"family": "Lee", "given": "Mikyeong", "initials": "M"}, {"family": "Baccarelli", "given": "Andrea A", "initials": "AA"}, {"family": "Whitsel", "given": "Eric A", "initials": "EA"}], "type": "journal article", "published": "2022-09-00", "journal": {"title": "Environ. Res.", "issn": "1096-0953", "volume": "212", "issue": "Pt C", "pages": "113360", "issn-l": "0013-9351"}, "abstract": "Epigenetic mechanisms may underlie air pollution-health outcome associations. We estimated gaseous air pollutant-DNA methylation (DNAm) associations using twelve subpopulations within Women's Health Initiative (WHI) and Atherosclerosis Risk in Communities (ARIC) cohorts (n = 8397; mean age 61.3 years; 83% female; 46% African-American, 46% European-American, 8% Hispanic/Latino). We used geocoded participant address-specific mean ambient carbon monoxide (CO), nitrogen oxides (NO2; NOx), ozone (O3), and sulfur dioxide (SO2) concentrations estimated over the 2-, 7-, 28-, and 365-day periods before collection of blood samples used to generate Illumina 450 k array leukocyte DNAm measurements. We estimated methylome-wide, subpopulation- and race/ethnicity-stratified pollutant-DNAm associations in multi-level, linear mixed-effects models adjusted for sociodemographic, behavioral, meteorological, and technical covariates. We combined stratum-specific estimates in inverse variance-weighted meta-analyses and characterized significant associations (false discovery rate; FDR<0.05) at Cytosine-phosphate-Guanine (CpG) sites without among-strata heterogeneity (PCochran's Q > 0.05). We attempted replication in the Cooperative Health Research in Region of Augsburg (KORA) study and Normative Aging Study (NAS). We observed a -0.3 (95% CI: -0.4, -0.2) unit decrease in percent DNAm per interquartile range (IQR, 7.3 ppb) increase in 28-day mean NO2 concentration at cg01885635 (chromosome 3; regulatory region 290 bp upstream from ZNF621; FDR = 0.03). At intragenic sites cg21849932 (chromosome 20; LIME1; intron 3) and cg05353869 (chromosome 11; KLHL35; exon 2), we observed a -0.3 (95% CI: -0.4, -0.2) unit decrease (FDR = 0.04) and a 1.2 (95% CI: 0.7, 1.7) unit increase (FDR = 0.04), respectively, in percent DNAm per IQR (17.6 ppb) increase in 7-day mean ozone concentration. Results were not fully replicated in KORA and NAS. We identified three CpG sites potentially susceptible to gaseous air pollution-induced DNAm changes near genes relevant for cardiovascular and lung disease. Further harmonized investigations with a range of gaseous pollutants and averaging durations are needed to determine the effect of gaseous air pollutants on DNA methylation and ultimately gene expression.", "doi": "10.1016/j.envres.2022.113360", "pmid": "35500859", "labels": [], "xrefs": [{"db": "mid", "key": "NIHMS1804648"}, {"db": "pmc", "key": "PMC9354583"}, {"db": "pii", "key": "S0013-9351(22)00687-9"}], "notes": [], "created": "2026-08-21T11:19:20.763Z", "modified": "2026-08-21T11:19:20.793Z"}