{"entity": "publication", "iuid": "dd218756da1f4e8482212a0a06a5712d", "timestamp": "2026-08-20T20:42:28.713Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/dd218756da1f4e8482212a0a06a5712d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/dd218756da1f4e8482212a0a06a5712d"}}, "title": "PAT-on-a-chip: Miniaturization of analytical assays towards data-driven bioprocess development and optimization.", "authors": [{"family": "Pinto", "given": "In\u00eas F", "initials": "IF"}, {"family": "Abeille", "given": "Fabien", "initials": "F"}, {"family": "Giehring", "given": "Sebastian", "initials": "S"}, {"family": "Akhtar", "given": "Ahmad S", "initials": "AS"}, {"family": "Sergeant", "given": "David", "initials": "D"}, {"family": "Chotteau", "given": "Veronique", "initials": "V"}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2025-10-15", "journal": {"title": "Biosens Bioelectron", "issn": "1873-4235", "volume": "286", "pages": "117625", "issn-l": "0956-5663"}, "abstract": "The advancement of biopharmaceutical manufacturing, particularly continuous processing, has heightened the need for next-generation analytical tools approaching real-time monitoring of critical quality attributes (CQAs) and process parameters (CPPs). Current methods, primarily offline and labor-intensive, fail at delivering analytical information that can be used for process analytical technology (PAT) to control and optimize the manufacturing process, while also lacking the ability of multi-attribute monitoring, thus requiring a large number of samples (or sampling amount) to be collected. This work introduces the concept of PAT-on-a-chip, consisting of an integrated microfluidic platform designed to perform at-line analysis and characterization of cell culture samples in the context of monoclonal antibody (mAb) production. Specifically, a sample preparation-free miniaturized lectin-based assay was developed to measure levels of high mannose glycans and integrated with affinity-based assays to measure mAb titers and key impurities, namely Chinese hamster ovary (CHO) host cell proteins (HCP), within the same chip, resorting to a common colorimetric readout. The microfluidic chips were operated in a customized and integrated instrument comprising miniaturized photodiodes, connected to a graphical user interface for data recording and signal quantification. The PAT-on-a-chip unit allowed to achieve fit-for-purpose analyte quantification, while offering performance comparable to state-of-the-art offline analytical methods (Pearson R > 0.93), namely capillary electrophoresis with laser-induced fluorescence (CE-LIF) for glycan analysis, well plate immunoassays for CHO HCP and protein A HPLC for mAb titers, thus validating its potential to expand the modern PAT toolbox.", "doi": "10.1016/j.bios.2025.117625", "pmid": "40435762", "labels": [], "xrefs": [{"db": "pii", "key": "S0956-5663(25)00499-3"}], "notes": [], "created": "2026-08-20T06:43:47.640Z", "modified": "2026-08-20T06:43:47.692Z"}