{"entity": "publication", "iuid": "cb74f05aafc44aa99ae8806784362ad8", "timestamp": "2026-08-23T22:11:53.695Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/cb74f05aafc44aa99ae8806784362ad8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/cb74f05aafc44aa99ae8806784362ad8"}}, "title": "Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease.", "authors": [{"family": "de Jong", "given": "Ymke A", "initials": "YA"}, {"family": "Seren", "given": "Rana M", "initials": "RM"}, {"family": "Ram\u0161ak Mar\u010deta", "given": "Vida", "initials": "V"}, {"family": "Checa", "given": "Antonio", "initials": "A"}, {"family": "Petursdott\u00edr", "given": "Dagbjort H", "initials": "DH"}, {"family": "Badolati", "given": "Isabella", "initials": "I"}, {"family": "Moeckel", "given": "Claudia", "initials": "C"}, {"family": "Ahmed Osman", "given": "Omneya", "initials": "O"}, {"family": "Hell", "given": "Eva", "initials": "E"}, {"family": "Huseby", "given": "Douglas L", "initials": "DL"}, {"family": "Hughes", "given": "Diarmaid", "initials": "D"}, {"family": "Wheelock", "given": "Craig E", "initials": "CE"}, {"family": "Garcia", "given": "Sarahi L", "initials": "SL"}, {"family": "Udekwu", "given": "Klas I", "initials": "KI"}, {"family": "Qazi", "given": "Khaleda R", "initials": "KR"}, {"family": "Sverremark-Ekstr\u00f6m", "given": "Eva", "initials": "E"}], "type": "journal article", "published": "2025-09-16", "journal": {"title": "BMC Microbiol.", "issn": "1471-2180", "volume": "25", "issue": "1", "pages": "575", "issn-l": "1471-2180"}, "abstract": "Human microbiota-associated (HMA) models are used to allow in vivo studies of the human gut microbiome and its effects on host physiology. In particular, alterations in early life microbiota have been linked to allergy development during childhood. In this study, we investigated how pools of human microbiota collected from infants with different allergy risk, thrive in mice and their offspring, as well as how they influence the host metabolome.\n\nWe used a two-generation HMA mouse model in which dams were colonized with human feces from three groups of infants (n = 19, samples collected during the first 8 weeks of life). In two of the groups, all infants had a strong hereditary risk for allergic disease (n = 12), but only 6 of them developed allergy before 2 years of age. In the third group, which was used as a control, none of the infants had allergic heredity or developed allergy (n = 7). Microbiota trajectories were followed from inoculation to mouse offspring, and metabolic profiles were monitored in several intestinal organs as well as in the serum of the murine offspring.\n\nThe human microbiota adapted to the murine host but still presented distinct compositional features, reflecting the original inoculated samples. These microbial differences were mirrored in the mouse offspring metabolome, with group-associated patterns in sphingolipids, acylcarnitines and tryptophan metabolites. Furthermore, the metabolic profiles of the mouse offspring aligned with those observed in fecal water preparations from the corresponding human infant fecal samples.\n\nOur findings highlight the significant impact of early-life microbiota on the host metabolome and show that our two-generation HMA model is suitable for studying microbiota\u2012metabolome relationships relevant to humans. The differences in microbiota\u2012metabolome correlations between individuals who develop or do not develop allergic disease suggest that an allergic predisposition might be more multifaceted than previously believed.", "doi": "10.1186/s12866-025-04321-9", "pmid": "40954473", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12439373"}, {"db": "pii", "key": "10.1186/s12866-025-04321-9"}, {"db": "BioProject", "key": "PRJNA1134143"}], "notes": [], "created": "2026-08-21T12:38:17.614Z", "modified": "2026-08-21T12:38:17.627Z"}