{"entity": "publication", "iuid": "c4b9cca27860453e84b806a7987f215d", "timestamp": "2026-08-20T20:50:05.986Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c4b9cca27860453e84b806a7987f215d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c4b9cca27860453e84b806a7987f215d"}}, "title": "The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy.", "authors": [{"family": "Liu", "given": "Jiangnan", "initials": "J", "orcid": "0000-0002-3086-7567", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c28ada139d64dca82657d1ee5f1d2b0.json"}}, {"family": "Nagy", "given": "Noemi", "initials": "N", "orcid": "0000-0002-7800-3493", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3f27465b7465457c89f55ef01194da02.json"}}, {"family": "Ayala-Torres", "given": "Carlos", "initials": "C", "orcid": "0000-0002-2306-6788", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/789af2aa7d6543a9bdb157d59dceb458.json"}}, {"family": "Bleuse", "given": "Solenne", "initials": "S"}, {"family": "Aguilar-Alonso", "given": "Francisco", "initials": "F", "orcid": "0000-0003-4457-5642", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bf76e002fbd046b1840cc6988e559ea8.json"}}, {"family": "Larsson", "given": "Ola", "initials": "O", "orcid": "0000-0003-1412-1308", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3f81c6cbbe564d38bc78d1af5153d804.json"}}, {"family": "Masucci", "given": "Maria G", "initials": "MG", "orcid": "0000-0002-5541-2809", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ac1411f252f403485d658f2c74204f0.json"}}], "type": "journal article", "published": "2025-05-00", "journal": {"title": "Autophagy", "issn": "1554-8635", "volume": "21", "issue": "5", "pages": "996-1018", "issn-l": "1554-8627"}, "abstract": "The synthesis of membrane and secreted proteins is safeguarded by an endoplasmic reticulum-associated ribosome quality control (ER-RQC) that promotes the disposal of defective translation products by the proteasome or via a lysosome-dependent pathway involving the degradation of portions of the ER by macroautophagy (reticulophagy). The UFMylation of RPL26 on ER-stalled ribosomes is essential for activating the ER-RQC and reticulophagy. Here, we report that the viral deubiquitinase (vDUB) encoded in the N-terminal domain of the Epstein-Barr virus (EBV) large tegument protein BPLF1 hinders the UFMylation of RPL26 on ribosomes that stall at the ER, promotes the stabilization of ER-RQC substrates, and inhibits reticulophagy. The vDUB did not act as a de-UFMylase or interfere with the UFMylation of the ER membrane protein CYB5R3 by the UFL1 ligase. Instead, it copurified with ribosomes in sucrose gradients and abrogated a ZNF598- and LTN1-independent ubiquitination event required for RPL26 UFMylation. Physiological levels of BPLF1 impaired the UFMylation of RPL26 in productively EBV-infected cells, pointing to an important role of the enzyme in regulating the translation quality control that allows the efficient synthesis of viral proteins and the production of infectious virus.Abbreviation: BPLF1, BamH1 P fragment left open readingframe-1; CDK5RAP3, CDK5regulatory subunit associated protein 3; ChFP, mCherry fluorescent protein; DDRGK1, DDRGKdomain containing 1; EBV, Epstein-Barr virus; eGFP, enhancedGFP; ER-RQC, endoplasmicreticulum-associated ribosome quality control; LCL, EBV-carryinglymphoblastoid cell line; GFP, green fluorescent protein; RQC, ribosome quality control; SRP, signal recognition particle; UFM1, ubiquitin fold modifier 1; UFL1, UFM1 specific ligase 1.", "doi": "10.1080/15548627.2024.2440846", "pmid": "39842454", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12013442"}], "notes": [], "created": "2026-08-20T09:37:01.535Z", "modified": "2026-08-20T09:37:01.762Z"}