{"entity": "publication", "iuid": "c2f76c44eb7f4e60b25591e87657cc4d", "timestamp": "2026-08-20T21:08:14.561Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c2f76c44eb7f4e60b25591e87657cc4d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c2f76c44eb7f4e60b25591e87657cc4d"}}, "title": "Caprin-1 binding to the critical stress granule protein G3BP1 is influenced by pH.", "authors": [{"family": "Schulte", "given": "Tim", "initials": "T"}, {"family": "Panas", "given": "Marc D", "initials": "MD"}, {"family": "Han", "given": "Xiao", "initials": "X", "orcid": "0000-0003-0879-4119", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/84f84bbd0b5a4d86b5512581773446ed.json"}}, {"family": "Williams", "given": "Lucy", "initials": "L"}, {"family": "Kedersha", "given": "Nancy", "initials": "N"}, {"family": "Fleck", "given": "Jonas Simon", "initials": "JS"}, {"family": "Tan", "given": "Timothy J C", "initials": "TJC"}, {"family": "Dopico", "given": "Xaquin Castro", "initials": "XC", "orcid": "0000-0002-9005-6774", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/473ffce480ca4f2e8f75ad7bb4097e17.json"}}, {"family": "Olsson", "given": "Anders", "initials": "A"}, {"family": "Morro", "given": "Ainhoa Moliner", "initials": "AM"}, {"family": "Hanke", "given": "Leo", "initials": "L", "orcid": "0000-0001-5514-2418", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1c38fa28e3d4733978f027a582c2230.json"}}, {"family": "Nilvebrant", "given": "Johan", "initials": "J"}, {"family": "Giang", "given": "Kim Anh", "initials": "KA"}, {"family": "Nygren", "given": "Per-\u00c5ke", "initials": "P\u00c5"}, {"family": "Anderson", "given": "Paul", "initials": "P"}, {"family": "Achour", "given": "Adnane", "initials": "A", "orcid": "0000-0003-0432-710X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fba324156a4fcbafbe636e642587a9.json"}}, {"family": "McInerney", "given": "Gerald M", "initials": "GM", "orcid": "0000-0003-2257-7241", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/012d1aaf306b43dc89309b45ccc6b649.json"}}], "type": "journal article", "published": "2023-05-00", "journal": {"title": "Open Biol", "issn": "2046-2441", "volume": "13", "issue": "5", "pages": "220369", "issn-l": "2046-2441"}, "abstract": "G3BP is the central node within stress-induced protein-RNA interaction networks known as stress granules (SGs). The SG-associated proteins Caprin-1 and USP10 bind mutually exclusively to the NTF2 domain of G3BP1, promoting and inhibiting SG formation, respectively. Herein, we present the crystal structure of G3BP1-NTF2 in complex with a Caprin-1-derived short linear motif (SLiM). Caprin-1 interacts with His-31 and His-62 within a third NTF2-binding site outside those covered by USP10, as confirmed using biochemical and biophysical-binding assays. Nano-differential scanning fluorimetry revealed reduced thermal stability of G3BP1-NTF2 at acidic pH. This destabilization was counterbalanced significantly better by bound USP10 than Caprin-1. The G3BP1/USP10 complex immunoprecipated from human U2OS cells was more resistant to acidic buffer washes than G3BP1/Caprin-1. Acidification of cellular condensates by approximately 0.5 units relative to the cytosol was detected by ratiometric fluorescence analysis of pHluorin2 fused to G3BP1. Cells expressing a Caprin-1/FGDF chimera with higher G3BP1-binding affinity had reduced Caprin-1 levels and slightly reduced condensate sizes. This unexpected finding may suggest that binding of the USP10-derived SLiM to NTF2 reduces the propensity of G3BP1 to enter condensates.", "doi": "10.1098/rsob.220369", "pmid": "37161291", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10170197"}, {"db": "figshare", "key": "10.6084/m9.figshare.c.6626084"}], "notes": [], "created": "2026-08-20T09:52:56.809Z", "modified": "2026-08-20T09:52:56.938Z"}