{"entity": "publication", "iuid": "c01601d0356846e6812a078ba0ab612b", "timestamp": "2026-08-26T05:17:01.496Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c01601d0356846e6812a078ba0ab612b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c01601d0356846e6812a078ba0ab612b"}}, "title": "Cold-Active Shewanella glacialimarina TZS-4T nov. Features a Temperature-Dependent Fatty Acid Profile and Putative Sialic Acid Metabolism.", "authors": [{"family": "Qasim", "given": "Muhammad Suleman", "initials": "MS"}, {"family": "Lampi", "given": "Mirka", "initials": "M"}, {"family": "Heinonen", "given": "Minna-Maria K", "initials": "MK"}, {"family": "Garrido-Zabala", "given": "Berta", "initials": "B"}, {"family": "Bamford", "given": "Dennis H", "initials": "DH"}, {"family": "K\u00e4kel\u00e4", "given": "Reijo", "initials": "R"}, {"family": "Roine", "given": "Elina", "initials": "E"}, {"family": "Sarin", "given": "Leif Peter", "initials": "LP"}], "type": "journal article", "published": "2021-10-01", "journal": {"title": "Front Microbiol", "issn": "1664-302X", "volume": "12", "pages": "737641", "issn-l": "1664-302X"}, "abstract": "Species of genus Shewanella are among the most frequently identified psychrotrophic bacteria. Here, we have studied the cellular properties, growth dynamics, and stress conditions of cold-active Shewanella strain #4, which was previously isolated from Baltic Sea ice. The cells are rod-shaped of ~2\u03bcm in length and 0.5\u03bcm in diameter, and they grow between 0 and 25\u00b0C, with an optimum at 15\u00b0C. The bacterium grows at a wide range of conditions, including 0.5-5.5% w/v NaCl (optimum 0.5-2% w/v NaCl), pH 5.5-10 (optimum pH 7.0), and up to 1mM hydrogen peroxide. In keeping with its adaptation to cold habitats, some polyunsaturated fatty acids, such as stearidonic acid (18:4n-3), eicosatetraenoic acid (20:4n-3), and eicosapentaenoic acid (20:5n-3), are produced at a higher level at low temperature. The genome is 4,456kb in size and has a GC content of 41.12%. Uniquely, strain #4 possesses genes for sialic acid metabolism and utilizes N-acetyl neuraminic acid as a carbon source. Interestingly, it also encodes for cytochrome c3 genes, which are known to facilitate environmental adaptation, including elevated temperatures and exposure to UV radiation. Phylogenetic analysis based on a consensus sequence of the seven 16S rRNA genes indicated that strain #4 belongs to genus Shewanella, closely associated with Shewanella aestuarii with a ~97% similarity, but with a low DNA-DNA hybridization (DDH) level of ~21%. However, average nucleotide identity (ANI) analysis defines strain #4 as a separate Shewanella species (ANI score=76). Further phylogenetic analysis based on the 92 most conserved genes places Shewanella strain #4 into a distinct phylogenetic clade with other cold-active marine Shewanella species. Considering the phylogenetic, phenotypic, and molecular characterization, we conclude that Shewanella strain #4 is a novel species and name it Shewanella glacialimarina sp. nov. TZS-4T, where glacialimarina means sea ice. Consequently, S. glacialimarina TZS-4T constitutes a promising model for studying transcriptional and translational regulation of cold-active metabolism.", "doi": "10.3389/fmicb.2021.737641", "pmid": "34659168", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8519357"}], "notes": [], "created": "2026-08-21T12:59:33.634Z", "modified": "2026-08-21T12:59:33.662Z"}