{"entity": "publication", "iuid": "ba39aa35d82d4bbea6ba59526c337675", "timestamp": "2026-09-28T23:49:58.603Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ba39aa35d82d4bbea6ba59526c337675.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ba39aa35d82d4bbea6ba59526c337675"}}, "title": "Image-Based Detection of Patient-Specific Drug-Induced Cell-Cycle Effects in Glioblastoma.", "authors": [{"family": "Matuszewski", "given": "Damian J", "initials": "DJ", "orcid": "0000-0002-6148-5174", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a5ffba35019240ac8f0e6daf089aec98.json"}}, {"family": "W\u00e4hlby", "given": "Carolina", "initials": "C"}, {"family": "Krona", "given": "Cecilia", "initials": "C"}, {"family": "Nelander", "given": "Sven", "initials": "S"}, {"family": "Sintorn", "given": "Ida-Maria", "initials": "IM"}], "type": "journal article", "published": "2018-12-00", "journal": {"title": "SLAS Discov", "issn": "2472-5560", "volume": "23", "issue": "10", "pages": "1030-1039", "issn-l": "2472-5552"}, "abstract": "Image-based analysis is an increasingly important tool to characterize the effect of drugs in large-scale chemical screens. Herein, we present image and data analysis methods to investigate population cell-cycle dynamics in patient-derived brain tumor cells. Images of glioblastoma cells grown in multiwell plates were used to extract per-cell descriptors, including nuclear DNA content. We reduced the DNA content data from per-cell descriptors to per-well frequency distributions, which were used to identify compounds affecting cell-cycle phase distribution. We analyzed cells from 15 patient cases representing multiple subtypes of glioblastoma and searched for clusters of cell-cycle phase distributions characterizing similarities in response to 249 compounds at 11 doses. We show that this approach applied in a blind analysis with unlabeled substances identified drugs that are commonly used for treating solid tumors as well as other compounds that are well known for inducing cell-cycle arrest. Redistribution of nuclear DNA content signals is thus a robust metric of cell-cycle arrest in patient-derived glioblastoma cells.", "doi": "10.1177/2472555218791414", "pmid": "30074852", "labels": [], "xrefs": [{"db": "pii", "key": "S2472-5552(22)06926-X"}], "notes": [], "created": "2026-09-23T14:58:51.380Z", "modified": "2026-09-23T14:58:51.434Z"}