{"entity": "publication", "iuid": "b137420f2f664605ad6829bbc1aabd85", "timestamp": "2026-04-20T03:38:45.347Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b137420f2f664605ad6829bbc1aabd85.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b137420f2f664605ad6829bbc1aabd85"}}, "title": "Transcriptional response to stress is pre-wired by promoter and enhancer architecture.", "authors": [{"family": "Vihervaara", "given": "Anniina", "initials": "A"}, {"family": "Mahat", "given": "Dig Bijay", "initials": "DB"}, {"family": "Guertin", "given": "Michael J", "initials": "MJ"}, {"family": "Chu", "given": "Tinyi", "initials": "T"}, {"family": "Danko", "given": "Charles G", "initials": "CG"}, {"family": "Lis", "given": "John T", "initials": "JT"}, {"family": "Sistonen", "given": "Lea", "initials": "L", "orcid": "0000-0003-1341-2867", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aafaee6a2aca4f4783add9775d660b74.json"}}], "type": "journal article", "published": "2017-08-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "8", "issue": "1", "pages": "255", "issn-l": "2041-1723"}, "abstract": "Programs of gene expression are executed by a battery of transcription factors that coordinate divergent transcription from a pair of tightly linked core initiation regions of promoters and enhancers. Here, to investigate how divergent transcription is reprogrammed upon stress, we measured nascent RNA synthesis at nucleotide-resolution, and profiled histone H4 acetylation in human cells. Our results globally show that the release of promoter-proximal paused RNA polymerase into elongation functions as a critical switch at which a gene's response to stress is determined. Highly transcribed and highly inducible genes display strong transcriptional directionality and selective assembly of general transcription factors on the core sense promoter. Heat-induced transcription at enhancers, instead, correlates with prior binding of cell-type, sequence-specific transcription factors. Activated Heat Shock Factor 1 (HSF1) binds to transcription-primed promoters and enhancers, and CTCF-occupied, non-transcribed chromatin. These results reveal chromatin architectural features that orient transcription at divergent regulatory elements and prime transcriptional responses genome-wide.Heat Shock Factor 1 (HSF1) is a regulator of stress-induced transcription. Here, the authors investigate changes to transcription and chromatin organization upon stress and find that activated HSF1 binds to transcription-primed promoters and enhancers, and to CTCF occupied, untranscribed chromatin.", "doi": "10.1038/s41467-017-00151-0", "pmid": "28811569", "labels": {"Anniina Vihervaara": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC5557961"}, {"db": "pii", "key": "10.1038/s41467-017-00151-0"}], "notes": [], "created": "2023-12-03T19:29:12.683Z", "modified": "2023-12-03T19:30:46.595Z"}