{"entity": "publication", "iuid": "abfd47840e4047e08184aa6e91723bd6", "timestamp": "2026-08-23T09:26:24.986Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/abfd47840e4047e08184aa6e91723bd6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/abfd47840e4047e08184aa6e91723bd6"}}, "title": "CD40 stimulation via CD40 ligand enhances adenovirus-mediated tumour immunogenicity including 'find-me', 'eat-me', and 'kill-me' signalling.", "authors": [{"family": "Naseri", "given": "Sedigheh", "initials": "S"}, {"family": "Cordova", "given": "Mariela Mejia", "initials": "MM"}, {"family": "Wenthe", "given": "Jessica", "initials": "J"}, {"family": "L\u00f6vgren", "given": "Tanja", "initials": "T"}, {"family": "Eriksson", "given": "Emma", "initials": "E"}, {"family": "Loskog", "given": "Angelica", "initials": "A", "orcid": "0000-0001-8583-6138", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/572e5b01b3bd48779252b9020732cb09.json"}}, {"family": "Ullenhag", "given": "Gustav J", "initials": "GJ"}], "type": "journal article", "published": "2024-04-00", "journal": {"title": "J. Cell. Mol. Med.", "issn": "1582-4934", "volume": "28", "issue": "7", "pages": "e18162", "issn-l": "1582-1838"}, "abstract": "Immunostimulatory gene therapy using oncolytic viruses is currently evaluated as a promising therapy for cancer aiming to induce anti-tumour immunity. Here, we investigate the capacity of oncolytic adenoviruses (LOAd) and their transgenes to induce immunogenicity in the infected tumour cells. Oncolysis and death-related markers were assessed after infection of eight human solid cancer cell lines with different LOAd viruses expressing a trimerized, membrane-bound (TMZ)-CD40L, TMZ-CD40L and 41BBL, or no transgenes. The viruses induced transgene expression post infection before they were killed by oncolysis. Death receptors TRAIL-R1, TRAIL-R2 and Fas as well as immunogenic cell death marker calreticulin were upregulated in cell lines post infection. Similarly, caspase 3/7 activity was increased in most cell lines. Interestingly, in CD40+ cell lines there was a significant effect of the TMZ-CD40L-encoding viruses indicating activation of the CD40-mediated apoptosis pathway. Further, these cell lines showed a significant increase of calreticulin, and TRAIL receptor 1 and 2 post infection. However, LOAd viruses induced PD-L1 upregulation which may hamper anti-tumour immune responses. In conclusion, LOAd infection increased the immunogenicity of infected tumour cells and this was potentiated by CD40 stimulation. Due to the simultaneous PD-L1 increase, LOAd viruses may benefit from combination with antibodies blocking PD1/PD-L1.", "doi": "10.1111/jcmm.18162", "pmid": "38494863", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10945091"}], "notes": [], "created": "2026-08-21T12:19:47.936Z", "modified": "2026-08-21T12:19:47.987Z"}