{"entity": "publication", "iuid": "aa2468a46a034a4eae4a94bb949beeac", "timestamp": "2026-08-20T20:45:16.387Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/aa2468a46a034a4eae4a94bb949beeac.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/aa2468a46a034a4eae4a94bb949beeac"}}, "title": "Protocol for image-based monitoring of de novo RNA synthesis at DNA double-strand breaks in human cell lines.", "authors": [{"family": "Pappas", "given": "Georgios", "initials": "G"}, {"family": "Gram", "given": "Helena Hagner", "initials": "HH"}, {"family": "Bartek", "given": "Jiri", "initials": "J"}, {"family": "Galanos", "given": "Panagiotis", "initials": "P"}], "type": "journal article", "published": "2025-12-19", "journal": {"title": "STAR Protoc", "issn": "2666-1667", "volume": "6", "issue": "4", "pages": "104157", "issn-l": null}, "abstract": "DNA double-strand breaks (DSBs) halt canonical transcription and simultaneously trigger local non-canonical RNA synthesis. Despite its significance, existing approaches to monitor this process are limited. Here, we present a protocol to monitor and quantify nascent transcription on DSBs in the nucleus. We describe steps for employing UV-A laser to induce site-specific DSBs. We then detail procedures for supplementation of the ablated cells with 5-bromouridine 5'-triphosphate (BrUTP) to label nascent RNA. For complete details on the use and execution of this protocol, please refer to Pappas et al.1.", "doi": "10.1016/j.xpro.2025.104157", "pmid": "41134671", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12590427"}, {"db": "pii", "key": "S2666-1667(25)00563-5"}], "notes": [], "created": "2026-08-20T08:06:48.031Z", "modified": "2026-08-20T08:06:48.072Z"}