{"entity": "publication", "iuid": "a5920a684af44225bd12055afab19960", "timestamp": "2026-09-25T23:05:15.736Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a5920a684af44225bd12055afab19960.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a5920a684af44225bd12055afab19960"}}, "title": "Elucidating microRNA-34a organisation within human Argonaute-2 by dynamic nuclear polarisation-enhanced magic angle spinning NMR.", "authors": [{"family": "Dasgupta", "given": "Rubin", "initials": "R", "orcid": "0000-0003-4505-7775", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ced11f38f5941008f1af07961f06df0.json"}}, {"family": "Becker", "given": "Walter", "initials": "W"}, {"family": "Petzold", "given": "Katja", "initials": "K", "orcid": "0000-0001-9470-0347", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c62bcc3f6a1e4616ab9f842a98885f72.json"}}], "type": "journal article", "published": "2024-10-28", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "52", "issue": "19", "pages": "11995-12004", "issn-l": "0305-1048"}, "abstract": "Understanding mRNA regulation by microRNA (miR) relies on the structural understanding of the RNA-induced silencing complex (RISC). Here, we elucidate the structural organisation of miR-34a, which is de-regulated in various cancers, in human Argonaute-2 (hAgo2), the effector protein in RISC. This analysis employs guanosine-specific isotopic labelling and dynamic nuclear polarisation (DNP)-enhanced Magic Angle Spinning (MAS) NMR. Homonuclear correlation experiments revealed that the non-A-form helical conformation of miR-34a increases when incorporated into hAgo2 and subsequently bound to SIRT1 mRNA compared to the free miR-34a or the free mRNA:miR duplex. The C8-C1' correlation provided a nucleotide-specific distribution of C2'- and C3'-endo sugar puckering, revealing the capture of diverse dynamic conformations upon freezing. Predominantly C3'-endo puckering was observed for the seed region, while C2'-endo conformation was found in the central region, with a mixture of both conformations elsewhere. These observations provide insights into the molecular dynamics underlying miR-mediated mRNA regulation and demonstrate that experiments conducted under cryogenic conditions, such as at 90 K, can capture and reveal frozen dynamic states, using methods like DNP-enhanced MAS NMR or Cryo-Electron Microscopy.", "doi": "10.1093/nar/gkae744", "pmid": "39228364", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11514488"}, {"db": "pii", "key": "7748944"}], "notes": [], "created": "2026-09-23T12:19:28.798Z", "modified": "2026-09-23T12:19:28.871Z"}