{"entity": "publication", "iuid": "a4b17ad714274d74a33972ede2fbeecd", "timestamp": "2026-10-01T11:57:48.500Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a4b17ad714274d74a33972ede2fbeecd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a4b17ad714274d74a33972ede2fbeecd"}}, "title": "Low-Cost Genome-Scale Phasing with Barcode-Linked Sequencing.", "authors": [{"family": "Redin", "given": "David", "initials": "D"}], "type": "journal article", "published": "2022-11-07", "journal": {"title": "Methods in molecular biology (Clifton, N.J.)", "issn": "1940-6029", "volume": "2590", "pages": "85-99", "issn-l": "1064-3745"}, "abstract": "Complete comprehension of clinically relevant variation among human genomes is likely only to come from sequencing platforms that are cost-efficient, and which feature both accurate base calling and long-range DNA phasing capability. The NGS revolution has struggled to meet the latter of these needs. Here we describe a protocol to address this limitation by preserving the molecular origin of short sequencing reads with an insignificant increase to sequencing costs. Whole haplotype-resolved genomes with megabase-scale phase blocks can be obtained with this method; offering researchers a unique opportunity to tackle the hurdles of de novo sequencing without being limited by a lack of resources.", "doi": "10.1007/978-1-0716-2819-5_6", "pmid": "36335494", "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T15:22:10.830Z", "modified": "2026-09-23T15:22:10.842Z"}