{"entity": "publication", "iuid": "a289ece2da0d4bf4ac8b3262b733a708", "timestamp": "2026-08-20T21:18:47.901Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a289ece2da0d4bf4ac8b3262b733a708.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a289ece2da0d4bf4ac8b3262b733a708"}}, "title": "Ultrasound-Based Scaffold-Free Core-Shell Multicellular Tumor Spheroid Formation.", "authors": [{"family": "Olofsson", "given": "Karl", "initials": "K", "orcid": "0000-0002-3976-3430", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0766218454224fc4902cb31f18455dc3.json"}}, {"family": "Carannante", "given": "Valentina", "initials": "V", "orcid": "0000-0003-4464-7087", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e39f4c75a0c1476d846ff7b15a5b17c5.json"}}, {"family": "Takai", "given": "Madoka", "initials": "M", "orcid": "0000-0002-6238-6717", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dfa9d1facf924a599e6ec3e9cbfa1b54.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Wiklund", "given": "Martin", "initials": "M", "orcid": "0000-0002-3247-1945", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a287459b32984379afc72c507bc44015.json"}}], "type": "journal article", "published": "2021-03-20", "journal": {"title": "Micromachines (Basel)", "issn": "2072-666X", "volume": "12", "issue": "3", "issn-l": "2072-666X"}, "abstract": "In cancer research and drug screening, multicellular tumor spheroids (MCTSs) are a popular model to bridge the gap between in vitro and in vivo. However, the current techniques to culture mixed co-culture MCTSs do not mimic the structural architecture and cellular spatial distribution in solid tumors. In this study we present an acoustic trapping-based core-shell MCTSs culture method using sequential seeding of the core and shell cells into microwells coated with a protein repellent coating. Scaffold-free core-shell ovarian cancer OVCAR-8 cell line MCTSs were cultured, stained, cleared and confocally imaged on-chip. Image analysis techniques were used to quantify the shell thickness (23.2 \u00b1 1.8 \u00b5m) and shell coverage percentage (91.2 \u00b1 2.8%). We also show that the shell thickness was evenly distributed over the MCTS cores with the exception of being slightly thinner close to the microwell bottom. This scaffold-free core-shell MCTSs formation technique and the analysis tools presented herein could be used as an internal migration assay within the MCTS or to form core-shell MCTS co-cultures to study therapy response or the interaction between tumor and stromal cells.", "doi": "10.3390/mi12030329", "pmid": "33804708", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8003921"}, {"db": "pii", "key": "mi12030329"}], "notes": [], "created": "2026-08-20T13:42:23.174Z", "modified": "2026-08-20T13:42:23.378Z"}