{"entity": "publication", "iuid": "a267fa7473da43ce8a43528185de457d", "timestamp": "2026-08-26T22:46:49.386Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a267fa7473da43ce8a43528185de457d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a267fa7473da43ce8a43528185de457d"}}, "title": "DC-SCRIPT affects mammary organoids branching morphogenesis by modulating the FGFR1-pERK signaling axis.", "authors": [{"family": "Tang", "given": "Chunling", "initials": "C"}, {"family": "van den Bijgaart", "given": "Renske J E", "initials": "RJE"}, {"family": "Looman", "given": "Maaike W G", "initials": "MWG"}, {"family": "Triantis", "given": "Vassilis", "initials": "V"}, {"family": "N\u00f8rskov S\u00f8ndergaard", "given": "Jonas", "initials": "J"}, {"family": "Ansems", "given": "Marleen", "initials": "M"}, {"family": "Adema", "given": "Gosse J", "initials": "GJ"}], "type": "journal article", "published": "2020-07-15", "journal": {"title": "Dev. Biol.", "issn": "1095-564X", "volume": "463", "issue": "2", "pages": "101-109", "issn-l": "0012-1606"}, "abstract": "Loss of expression of the transcription regulator DC-SCRIPT (Zfp366) is a prominent prognostic event in estrogen receptor-positive breast cancer patients. Studying the inherent link between breast morphogenesis and tumorigenesis, we recently reported that DC-SCRIPT affects normal mammary branching morphogenesis and mammary epithelium homeostasis. Here we investigated the molecular mechanism involved in DC-SCRIPT mediated regulation of FGF2 induced mammary branching morphogenesis in a 3D organoid culture system. Our data show that the delayed mammary organoid branching observed in DC-SCRIPT-/- organoids cannot be compensated for by increasing FGF2 levels. Interestingly, FGFR1, the dominant FGF2 receptor, was expressed at a significantly lower level in basal epithelial cells of DC-SCRIPT deficient organoids relative to wildtype organoids. A potential link between DC-SCRIPT and FGFR1 was further supported by the predicted locations of the DC-SCRIPT DNA binding motif at the Fgfr1 gene. Moreover, ERK1/2 phosphorylation downstream of the FGFR1 pathway was decreased in basal epithelial cells of DC-SCRIPT deficient organoids. Altogether, this study shows a relationship between DC-SCRIPT and FGFR1 related pERK signaling in modulating the branching morphogenesis of mammary organoids in vitro.", "doi": "10.1016/j.ydbio.2020.05.001", "pmid": "32422143", "labels": [], "xrefs": [{"db": "pii", "key": "S0012-1606(20)30137-8"}], "notes": [], "created": "2026-08-21T11:31:11.396Z", "modified": "2026-08-21T11:31:11.423Z"}