{"entity": "publication", "iuid": "9a364201be9a48ed9ca77152e18a2f74", "timestamp": "2026-08-23T10:59:50.929Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9a364201be9a48ed9ca77152e18a2f74.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9a364201be9a48ed9ca77152e18a2f74"}}, "title": "ER-localized Shr3 is a selective co-translational folding chaperone necessary for amino acid permease biogenesis.", "authors": [{"family": "Myronidi", "given": "Ioanna", "initials": "I", "orcid": "0009-0002-3935-7886", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5a4d8f40c13c4036a43df4348544afdb.json"}}, {"family": "Ring", "given": "Andreas", "initials": "A", "orcid": "0009-0005-7590-827X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/824ad42d0b4641c992e6e3fe7a866e0b.json"}}, {"family": "Wu", "given": "Fei", "initials": "F", "orcid": "0000-0002-0120-7586", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab3c51eb8ec34ecb86ec4b5ea576507d.json"}}, {"family": "Ljungdahl", "given": "Per O", "initials": "PO", "orcid": "0000-0002-6625-3540", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/683fcaec33bf44159f021fde794c746c.json"}}], "type": "journal article", "published": "2023-09-04", "journal": {"title": "J. Cell Biol.", "issn": "1540-8140", "volume": "222", "issue": "9", "issn-l": "0021-9525"}, "abstract": "Proteins with multiple membrane-spanning segments (MS) co-translationally insert into the endoplasmic reticulum (ER) membrane of eukaryotic cells. Shr3, an ER membrane-localized chaperone in Saccharomyces cerevisiae, is required for the functional expression of a family of 18 amino acid permeases (AAP) comprised of 12 MS. We have used comprehensive scanning mutagenesis and deletion analysis of Shr3 combined with a modified split-ubiquitin approach to probe chaperone-substrate interactions in vivo. Shr3 selectively interacts with nested C-terminal AAP truncations in marked contrast to similar truncations of non-Shr3 substrate sugar transporters. Shr3-AAP interactions initiate with the first four MS of AAP and successively strengthen but weaken abruptly when all 12 MS are present. Shr3-AAP interactions are based on structural rather than sequence-specific interactions involving membrane and luminal domains of Shr3. The data align with Shr3 engaging nascent N-terminal chains of AAP, functioning as a scaffold to facilitate folding as translation completes.", "doi": "10.1083/jcb.202208060", "pmid": "37477900", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10359922"}, {"db": "pii", "key": "214248"}], "notes": [], "created": "2026-08-20T09:38:00.519Z", "modified": "2026-08-20T09:38:00.745Z"}