{"entity": "publication", "iuid": "9a2e8397da6449db90db33c3ba85c439", "timestamp": "2026-08-26T22:58:16.828Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9a2e8397da6449db90db33c3ba85c439.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9a2e8397da6449db90db33c3ba85c439"}}, "title": "Accurate detection of Newcastle disease virus using proximity-dependent DNA aptamer ligation assays.", "authors": [{"family": "Marnissi", "given": "Boutheina", "initials": "B", "orcid": "0000-0002-2782-6502", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/83aea1da31cf4eaa90ace4e13fbbf167.json"}}, {"family": "Khalfaoui", "given": "Khouloud", "initials": "K"}, {"family": "Ebai", "given": "Tonge", "initials": "T"}, {"family": "Marques Souza de Oliveira", "given": "Felipe", "initials": "F"}, {"family": "Ghram", "given": "Abdeljelil", "initials": "A"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Hmila", "given": "Issam", "initials": "I"}], "type": "journal article", "published": "2021-04-00", "journal": {"title": "FEBS Open Bio", "issn": "2211-5463", "volume": "11", "issue": "4", "pages": "1122-1131", "issn-l": "2211-5463"}, "abstract": "Detecting viral antigens at low concentrations in field samples can be crucial for early veterinary diagnostics. Proximity ligation assays (PLAs) in both solution and solid-phase formats are widely used for high-performance protein detection in medical research. However, the affinity reagents used, which are mainly poly- and monoclonal antibodies, play an important role in the performance of PLAs. Here, we have established the first homogeneous and solid-phase proximity-dependent DNA aptamer ligation assays for rapid and accurate detection of Newcastle disease virus (NDV). NDV is detected by a pair of extended DNA aptamers that, upon binding in proximity to proteins on the envelope of the virus, are joined by enzymatic ligation to form a unique amplicon that can be sensitively detected using real-time PCR. The sensitivity, specificity, and reproducibility of the assays were validated using 40 farm samples. The results demonstrated that the developed homogeneous and solid-phase PLAs, which use NDV-selective DNA aptamers, are more sensitive than the sandwich enzymatic-linked aptamer assay (ELAA), and have a comparable sensitivity to real-time reverse transcription PCR (rRT-PCR) as the gold standard detection method. In addition, the solid-phase PLA was shown to have a greater dynamic range with improved lower limit of detection, upper- and lower limit of quantification, and minimal detectable dose as compared with those of ELAA and rRT-PCR. The specificity of PLA is shown to be concordant with rRT-PCR.", "doi": "10.1002/2211-5463.13117", "pmid": "33595202", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8016122"}], "notes": [], "created": "2026-08-20T06:27:12.996Z", "modified": "2026-08-20T06:27:13.074Z"}