{"entity": "publication", "iuid": "9a2bd941867f477a9ffe16f60d8b3334", "timestamp": "2026-09-30T22:58:52.182Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9a2bd941867f477a9ffe16f60d8b3334.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9a2bd941867f477a9ffe16f60d8b3334"}}, "title": "Multi-cellular phenotypic dynamics during the progression of an immunocompetent breast cancer model.", "authors": [{"family": "Gsell", "given": "Louise", "initials": "L"}, {"family": "Watson", "given": "Spencer S", "initials": "SS"}, {"family": "Sutevski", "given": "Iva", "initials": "I"}, {"family": "Massara", "given": "Matteo", "initials": "M"}, {"family": "Soukup", "given": "Klara", "initials": "K"}, {"family": "Eroglu", "given": "Alper", "initials": "A"}, {"family": "Mold", "given": "Jeff E", "initials": "JE"}, {"family": "Cougnoux", "given": "Antony", "initials": "A"}, {"family": "Joyce", "given": "Johanna A", "initials": "JA"}, {"family": "Hausser", "given": "Jean", "initials": "J"}], "type": "journal article", "published": "2025-11-21", "journal": {"title": "iScience", "issn": "2589-0042", "volume": "28", "issue": "11", "pages": "113808", "issn-l": null}, "abstract": "The breast tumor microenvironment (TME) has recently been profiled at high resolution by performing single-cell RNA sequencing (scRNAseq) on patient samples. However, from patients' samples, analyzing the temporal dynamics of the TME is ethically, practically and scientifically challenging. Revealing these dynamics could structure inter-tumor heterogeneity into a temporally ordered sequence of causes and consequences in cellular events. Here, we survey the dynamics of the TME by performing scRNAseq at different time points of the progression of a mouse breast tumor allograft model driven by the PyMT antigen. We find that multi-cellular phenotypic dynamics follow one of three possible temporal patterns: stable colonization, wave-like, or progressive increase. In particular, IFN-responsive cancer cells, GzmB+ cytotoxic T cells, as well as C1q macrophages, increase in parallel with tumor progression. These findings establish the single-cell types and phenotypes in a progressing breast tumor, and reveal when these cellular players enter and leave the TME.", "doi": "10.1016/j.isci.2025.113808", "pmid": "41280683", "labels": {"SciLifeLab Fellow": "", "Jean Hausser": ""}, "xrefs": [{"db": "pmc", "key": "PMC12637086"}, {"db": "pii", "key": "S2589-0042(25)02069-3"}], "notes": [], "created": "2026-09-23T12:33:45.408Z", "modified": "2026-09-23T12:33:45.473Z"}